Voltage imaging of astrocyte-neuron interactions

项目摘要 本申请是对R 01 NS 113499“星形细胞的电压成像- 神经元的相互作用”的博士克里斯杜拉在塔夫茨大学。候选人是杰奎琳·加西亚，一位前... Dulla博士实验室的博士生，专注于星形胶质细胞电压成像和星形胶质细胞膜如何 电压影响谷氨酸摄取。杰奎琳将利用杜拉实验室目前使用的工具， 追求她的兴趣在创伤性脑损伤如何影响星形胶质细胞膜电位和下游 谷氨酸信号与R 01项目官员Miriam Leenders博士的讨论认为， “在范围内”，并将提供杰奎琳与发射台为她自己的独立职业生涯。杰奎琳有资格 作为一个拉丁裔的多样性候选人，由于她的经济背景不利。因此，她是一个 多个代表性不足群体的成员。这个补充将资助杰奎琳3年的毕业 论文工作。Y3的资金将取决于在Y2期间提交NRSA申请。在此期间 杰奎琳将接受细胞和分子神经科学，创伤性脑损伤，癫痫，神经胶质细胞 生物学，更多。她将获得生物化学，创伤性脑损伤模型，星形胶质细胞遗传学方面的专业知识。 编码电压指示器（GEVI）成像、iGluSnFR谷氨酸成像和电生理学。本次培训 将引导她走向NRSA应用程序和未来作为神经科学研究的领导者。加西亚女士 致力于创伤性脑损伤的学术研究杜拉医生对 指导学生，被授予2018年兰迪斯杰出导师奖，并参与了 HHMI Gilliam奖学金通过实验室的其他学生。这里列出的项目将为加西亚女士带来出色的表现 以及在谷氨酸神经传递、神经元/神经胶质相互作用和尖端成像方面的严格培训 方式。Dulla博士和Garcia女士开发了一种结构化，严格和定制的指导， 培训计划。通过这一补充提供的资金将使杰奎琳有机会丰富她 研究生培训，使她可以获得额外的培训，并建立一个广泛的专业网络， 同事这种多样性补充将给杰奎琳一个明确的道路走向一个令人兴奋的未来， 未来的研究领域的癫痫，创伤性脑损伤，谷氨酸神经传递。的活动 制定和丰富的机会将是至关重要的加西亚女士的发展和未来作为一个成功的 独立调查员

PROJECT SUMMARY This application is for a diversity supplement to the R01 NS113499 “VOLTAGE IMAGING OF ASTROCYTE- NEURON INTERACTIONS” of Dr. Chris Dulla at Tufts University. The candidate is Jacqueline Garcia, a pre- doctoral student in the lab of Dr. Dulla focusing on astrocyte voltage imaging and how astrocyte membrane voltage affects glutamate uptake. Jacqueline will take the tools currently in use the Dulla Lab and use them to pursue her interests in how traumatic brain injury affects astrocyte membrane potential and downstream glutamate signaling. Discussions with the R01 program officer, Dr. Miriam Leenders, deemed this approach was “in scope” and will provide Jacqueline with a launch pad for her own independent career. Jacqueline qualifies as a diversity candidate as a Latina and due to her economically disadvantaged background. Therefore she is a member of multiple underrepresented groups. This supplement will fund Jacqueline for 3 years of her graduate thesis work. Funding for Y3 will be contingent on submission of an NRSA application during Y2. During this time Jacqueline will receive training in cellular and molecular neuroscience, traumatic brain injury, epilepsy, glial biology, and more. She will gain expertise in biochemistry, models of traumatic brain injury, astrocyte genetically encoded voltage indicator (GEVI) imaging, iGluSnFR glutamate imaging, and electrophysiology. This training will guide her towards an NRSA application and a future as a leader in neuroscience research. Ms. Garcia is devoted to academic research with a focus on traumatic brain injury. Dr. Dulla has a great deal of experience mentoring students, was awarded the 2018 Landis Award for Outstanding Mentorship, and is involved with the HHMI Gilliam Fellowship through other students in the lab. The project laid out here will give Ms. Garcia excellent and rigorous training in glutamate neurotransmission, neuron/glia interactions, and cutting-edge imaging modalities. Dr. Dulla and Ms. Garcia have developed a structured, rigorous, and customized mentorship and training plan. The funding offered through this supplement will give Jaqueline the opportunity to enrich her graduate training so that she can gain additional training and create an extensive network of professional colleagues. This diversity supplement will give Jacqueline a clear path toward an exciting future as a up and coming researcher in the field of epilepsy, traumatic brain injury, and glutamate neurotransmission. The activities laid out and the enrichment opportunities will be critical to Ms. Garcia’s development and future as a successful independent investigator.