Husbandry and Transgenics Core

摘要/摘要-畜牧业和转基因核心 该核心将通过开发大规模培养Bergia和U19的新方法来支持U19的需求 Aiptasia动物，并通过提供Bergia表达荧光融合传感器，从注射的mRNA或从 DNA，通过转基因。虽然转基因在动物基因组中的插入、编辑和稳定表达 对现代生物学产生了革命性的影响，这是限制海洋动物继续应用的主要因素 在生物医学研究中，缺乏操纵基因功能的遗传工具。这个核心将开发出几个 为Bergia Brain Project的研究人员提供野生型和转基因动物的创新。因此， CORE将开发几个必要的工具，以将Bergia用作真正的海洋模型系统 神经科学。

SUMMARY / ABSTRACT – Husbandry and Transgenics Core The core will support the needs of the U19 by developing new methods for large scale culture of Berghia and Aiptasia animals, and by supplying Berghia expressing fluorescent fusion sensors, from injected mRNA or from DNA, through transgenesis. Although insertion, editing and stable expression of transgenes into animal genomes has had a transformative impact on modern biology, a major factor limiting ongoing application of marine animals in biomedical research is the lack of genetic tools for manipulating gene function. This core will develop several innovations to supply researchers in the Berghia Brain Project with wildtype and transgenic animals. Thus, the core will develop several tools necessary for the use of Berghia as a bona fide marine model system for neuroscience.