STnc520, a virulence-associated regulatory RNA in Salmonella Typhimurium

摘要 鼠伤寒沙门氏菌是美国和全世界食源性疾病的主要原因。在.期间 在感染初期，鼠伤寒沙门氏菌侵袭肠道上皮细胞。所需基因的表达 侵袭受一组转录因子的控制，这些转录因子编码有SPI-1致病岛。我们有 最近绘制了这些转录因子的调控靶点。大多数监管相互作用 我们确认了之前没有描述过的。因此，与入侵相关的监管网络是 比之前想象的要复杂得多。此外，我们的工作强烈表明了一个重要的角色 用于入侵过程中的调控RNA。特别令人感兴趣的是调节RNA STnc520，其表达是 与其他入侵基因的基因协调，并已被认为是建立一个 基于高通量转座子筛选的数据，在三个动物模型中的感染。在这里，我们将 确定STnc520在体外和体内对鼠伤寒沙门氏菌侵袭上皮细胞的需求。 我们还将确定STnc520的直接监管目标。因此，我们将在此期间确定STnc520的角色 入侵，为未来对该RNA和其他促进入侵的调控RNA的研究奠定了基础。

SUMMARY Salmonella enterica serovar Typhimurium is a major cause of foodborne illness in the USA and worldwide. During the initial stages of infection, S. Typhimurium invades gut epithelial cells. Expression of genes required for invasion is controlled by a group of transcription factors encoded with the SPI-1 pathogenicity island. We have recently mapped the regulatory targets of these transcription factors. The majority of the regulatory interactions we identified have not been previously described. Thus, the regulatory network associated with invasion is considerably more complex than previously appreciated. Moreover, our work strongly suggests an important role for regulatory RNAs during invasion. Of particular interest is the regulatory RNA STnc520, whose expression is coordinated with that of other invasion genes, and which has been suggested to be important for establishing an infection in three animal models, based on data from high-throughput transposon screens. Here, we will determine the requirement for STnc520 for S. Typhimurium to invade epithelial cells, both in vitro and in vivo. We will also identify direct regulatory targets of STnc520. Thus, we will establish the role of STnc520 during invasion, laying the foundation for future studies of this and other regulatory RNAs that promote invasion.