The Therapeutic Potential of Pasteurized Human Donor Breast Milk Exosomes

项目摘要：外体是一种小的、自然产生的纳米囊泡，可以促进细胞 沟通。外切体现在被认为是多种疾病的潜在治疗剂 器官系统。我们的初步数据显示，人类母乳衍生的外切体(HBMDE) 通过减少炎症和UP保护肠上皮细胞免受氧化应激的影响 调节保护蛋白。尽管这些结果很有希望，但并不是所有的早产儿 有机会获得自己母亲母乳的治疗益处。目前， 建议感染和预防传染病，防止分享新鲜食品 另一名婴儿母亲的母乳。母乳捐赠者银行已经在很大程度上解决了这个问题 问题是允许婴儿获得无病原体的巴氏杀菌母乳。最近的证据 表明在巴氏杀菌的供者牛奶中存在外切体，但目前还不知道它们是否存在 未经巴氏灭菌的新鲜人奶的相同疗效和免疫学特征 外显体。到目前为止，exosome还没有被用作系统性多器官疗法来减少 细胞损伤。捐献乳外切体的潜在用途将大大加快这一进程。 新的治疗策略。这项建议将提供关于 巴氏杀菌HBMDE的免疫原性和治疗特性。

Project Summary: Exosomes are small, naturally occurring nanovesicles that facilitate cellular communication. Exosomes are now being viewed as potential therapeutic agents in multiple organ systems. Our preliminary data show that human breast milk derived exosomes (HBMDE) protect intestinal epithelial cells from oxidative stress by decreasing inflammation and up regulating protective proteins. Although these results are promising, not all premature infants have access to the therapeutic benefit of their own mother’s breast milk. Currently the recommendation for infection and communicable disease prevention prevent sharing of fresh breast milk from another infant’s mother. Breast milk donor banks have largely addressed this problem by allowing infants access to pasteurized pathogen free breast milk. Recent evidence suggest that exosomes are present in pasteurized donor milk, but it is not known if they have the same therapeutic benefit and immune profile of non-pasteurized fresh human milk exosomes. To date, exosomes have not been used as a systemic multi-organ therapy to reduce cellular injury. The potential use of donor milk exosomes would greatly accelerate this relatively new treatment strategy. This proposal will provide a comprehensive analysis on the immunogenic and therapeutic properties of pasteurized HBMDE.