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The Therapeutic Potential of Pasteurized Human Donor Breast Milk Exosomes

巴氏灭菌人类捐赠母乳外泌体的治疗潜力

基本信息

批准号：
10313756
负责人：
Colin A Martin
金额：
$ 22.28万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10313756
关键词：
Activities of Daily Living Address Animal Model Biodistribution Biological Models Brain Breastfed infant Cell Culture Techniques Cell Proliferation Cell Survival Clinical Communicable Diseases Communication Data Development Disease Drug Kinetics Electrons Enzyme-Linked Immunosorbent Assay Epithelial Cells Excision Functional disorder Genes Homeostasis Human Human Milk Immune Immunotherapeutic agent In Vitro Infant Infection Inflammation Injury Intestines Magnetic Resonance Imaging Milk Banks Modeling Mothers Necrotizing Enterocolitis Neonatal Newborn Infant Organ Outcome Oxidative Stress Premature Infant Process Property Proteins Recommendation Risk Source Structure Testing Therapeutic Therapeutic Agents Tissues Vision Western Blotting base body system cell injury disorder prevention donor milk exosome gain of function immunogenic improved in vivo intestinal epithelium intestinal injury mortality mouse model nano nanovesicle neonate novel oxidative damage pasteurization pathogen prevent systemic inflammatory response therapeutic evaluation tool translational study treatment strategy unpasteurized

项目摘要

Project Summary: Exosomes are small, naturally occurring nanovesicles that facilitate cellular communication. Exosomes are now being viewed as potential therapeutic agents in multiple organ systems. Our preliminary data show that human breast milk derived exosomes (HBMDE) protect intestinal epithelial cells from oxidative stress by decreasing inflammation and up regulating protective proteins. Although these results are promising, not all premature infants have access to the therapeutic benefit of their own mother’s breast milk. Currently the recommendation for infection and communicable disease prevention prevent sharing of fresh breast milk from another infant’s mother. Breast milk donor banks have largely addressed this problem by allowing infants access to pasteurized pathogen free breast milk. Recent evidence suggest that exosomes are present in pasteurized donor milk, but it is not known if they have the same therapeutic benefit and immune profile of non-pasteurized fresh human milk exosomes. To date, exosomes have not been used as a systemic multi-organ therapy to reduce cellular injury. The potential use of donor milk exosomes would greatly accelerate this relatively new treatment strategy. This proposal will provide a comprehensive analysis on the immunogenic and therapeutic properties of pasteurized HBMDE.

项目概述：外泌体是小的，天然存在的纳米囊泡，通信外泌体现在被视为多种疾病的潜在治疗剂。器官系统。我们的初步数据显示，人类母乳来源的外泌体（HBMDE）保护肠上皮细胞免受氧化应激通过减少炎症和向上调节保护性蛋白。虽然这些结果是有希望的，但并不是所有的早产儿都能从自己母亲的母乳中获得治疗效果。目前预防感染及传染病的建议另一个婴儿母亲的母乳母乳捐赠银行在很大程度上解决了这一问题通过允许婴儿获得经过巴氏消毒的无病原体母乳来解决这个问题。最近的证据表明外泌体存在于巴氏消毒的供体乳汁中，但尚不清楚它们是否与未经巴氏消毒新鲜人乳相同的治疗益处和免疫特性外来体迄今为止，外泌体尚未被用作全身性多器官疗法以减少肿瘤的发生。细胞损伤供体乳汁外泌体的潜在使用将大大加速这一相对新的治疗策略。该提案将对以下问题进行全面分析：巴氏灭菌HBMDE的免疫原性和治疗特性。