Non-invasive analysis of methylated cell free DNA in necrotizing enterocolitis
坏死性小肠结肠炎甲基化细胞游离 DNA 的无创分析
基本信息
- 批准号:10316733
- 负责人:
- 金额:$ 68.7万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-08-12 至 2021-11-30
- 项目状态:已结题
- 来源:
- 关键词:AbdomenAbdominal RadiographyAffectAge of OnsetAirAntibioticsBiologicalBiological AssayBiological MarkersBiologyBloodBlood specimenCase-Control StudiesCell LineageChemicalsClinicalClinical DataComplicationDNADNA MethylationDataDetectionDevelopmentDiagnosisDiagnosticDiagnostic ProcedureDiseaseEarly DiagnosisEconomic BurdenEnterocytesEnvironmental Risk FactorEpigenetic ProcessExcisionFecesGastrointestinal DiseasesGeneticGenetic TranscriptionGrowthHematopoieticHypermethylationInfantInfectionInflammatory ResponseIntestinesLasersLeukocytesMapsMetadataMethodsMethylationMorbidity - disease rateNecrosisNecrotizing EnterocolitisNeonatalNon-Invasive Cancer DetectionOnset of illnessOperative Surgical ProceduresOrgan failurePathogenesisPathogenicityPathologicPathway interactionsPhenotypePredispositionPremature InfantPremature Infant DiseasesRegimenRegulationResectedResourcesRiskSamplingScreening procedureSpecimenTestingTimebasebiobankbisulfite sequencingcell free DNAcourse developmentdevelopmental diseaseepigenomeepigenomicsfeedinggenome-widegenomic datahigh riskhuman tissueimprovedinnovationinsightintestinal injuryliquid biopsymedication administrationmicrobiomemolecular phenotypemortalitymouse modelneonatal micenovelnovel markerpostnatal developmentprecision medicinepredictive toolsprematurepreventprognostic toolprospectivescreeningsingle-cell RNA sequencingstool sampletranscriptomicswhole genome
项目摘要
Project Summary/Abstract
Necrotizing enterocolitis (NEC) is a devastating complication of prematurity that develops suddenly and carries
significant morbidity and a high mortality rate for which no biomarkers exist. The high mortality rate seen in NEC
could likely be prevented if there was a screening method that permitted early disease detection. Predictive and
prognostic tools for NEC are essential to advance our biological insight with a view towards non-invasive
detection and accurate and timely phenotyping. We have developed a NEC Biorepository with a variety of
specimen types and extensive clinical metadata obtained prospectively from premature infants before the
development of NEC. These samples allow us to interrogate the mechanisms that underlie NEC development,
including genetic or environmental factors. These factors include epigenetic mechanisms that regulate important
aspects of cellular differentiation and gut development. Importantly, the epigenetic mechanisms, specifically, at
the level of DNA methylation that take place during neonatal NEC are unknown. Our preliminary epigenomic
data demonstrate that numerous biological pathways are altered during NEC. Based on these findings, we now
hypothesize that non-invasive identification of methylation signatures can identify an infant at risk for necrotizing
enterocolitis. We will test this hypothesis by defining and quantifying the DNA methylation signatures in stool and
blood of premature infants before, during and after the development of NEC. Moreover, we will expand on our
observations that dysregulation of multiple key pathways is a defining pathogenic feature of NEC and use our
mouse model to gain additional mechanistic insights into NEC pathobiology. By the conclusion of the proposal,
we will have generated a considerable amount of genomic data from infants across the time course of
development and manifestation of NEC, and also during the normal extrauterine development of preterm infants.
These studies will advance our understanding of the epigenomic regulation of the pathways involved during NEC
development, and furthermore, may explain the unique susceptibility of the premature infant to NEC and identify
a non-invasive approach to diagnosing this devastating disease.
项目总结/文摘
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MISTY L GOOD其他文献
MISTY L GOOD的其他文献
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{{ truncateString('MISTY L GOOD', 18)}}的其他基金
Neonatal gut-on-a-chip platform for high content drug testing and precision medicine
用于高内涵药物测试和精准医学的新生儿肠道芯片平台
- 批准号:
10491072 - 财政年份:2022
- 资助金额:
$ 68.7万 - 项目类别:
Neonatal gut-on-a-chip platform for high content drug testing and precision medicine
用于高内涵药物测试和精准医学的新生儿肠道芯片平台
- 批准号:
10594705 - 财政年份:2022
- 资助金额:
$ 68.7万 - 项目类别:
Neonatal gut-on-a-chip platform for high content drug testing and precision medicine
用于高内涵药物测试和精准医学的新生儿肠道芯片平台
- 批准号:
10674890 - 财政年份:2022
- 资助金额:
$ 68.7万 - 项目类别:
Non-invasive analysis of methylated cell free DNA in necrotizing enterocolitis
坏死性小肠结肠炎甲基化细胞游离 DNA 的无创分析
- 批准号:
10704229 - 财政年份:2021
- 资助金额:
$ 68.7万 - 项目类别:
Non-invasive analysis of methylated cell free DNA in necrotizing enterocolitis
坏死性小肠结肠炎甲基化细胞游离 DNA 的无创分析
- 批准号:
10577705 - 财政年份:2021
- 资助金额:
$ 68.7万 - 项目类别:
Modulation of the Intestinal Immune Response in Necrotizing Enterocolitis
坏死性小肠结肠炎肠道免疫反应的调节
- 批准号:
10543597 - 财政年份:2018
- 资助金额:
$ 68.7万 - 项目类别:
Modulation of the Intestinal Immune Response in Necrotizing Enterocolitis
坏死性小肠结肠炎肠道免疫反应的调节
- 批准号:
10468084 - 财政年份:2018
- 资助金额:
$ 68.7万 - 项目类别:
Modulation of the Intestinal Immune Response in Necrotizing Enterocolitis
坏死性小肠结肠炎肠道免疫反应的调节
- 批准号:
10001502 - 财政年份:2018
- 资助金额:
$ 68.7万 - 项目类别:
Aryl hydrocarbon receptor signaling in the pathogenesis of necrotizing enterocolitis
坏死性小肠结肠炎发病机制中的芳基烃受体信号传导
- 批准号:
9220912 - 财政年份:2017
- 资助金额:
$ 68.7万 - 项目类别:
Novel Anti-Inflammatory Properties of Breast Milk in Necrotizing Enterocolitis
母乳在坏死性小肠结肠炎中的新型抗炎特性
- 批准号:
8820360 - 财政年份:2014
- 资助金额:
$ 68.7万 - 项目类别:














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