Shared Resource: Immune Monitoring & Flow Cytometry

项目总结：免疫监测与流式细胞仪共享资源(IM&FCSR) 免疫监测和流式细胞术共享资源(IM&FCSR)为研究人员提供 服务和研究专业知识，支持广泛的癌症实验室和临床研究 免疫学和免疫疗法、移植生物学、传染病和疫苗开发。这个 IM和FCSR生产定制的特定多肽和主要组织相容性的四聚体复合体 作为独特试剂的复杂分子用于评估抗原特异性T细胞并分离这些T细胞以用于 治疗试验。我们的流式细胞仪服务提供广泛的仪器，从单激光台式 从分析仪到复杂的多激光细胞分选仪。我们提供特定于协议的流式细胞仪免疫监测 以支持免疫疗法试验。我们量化细胞因子蛋白，作为免疫和其他细胞的衡量标准 功能，在高多样性、单电池和/或超灵敏的技术平台上。我们执行的是特定的 用于实验室研究的天然和转基因T细胞的细胞和分子分析 用于监测临床样本。按原样为数据评估和解释提供技术援助 协助优化实验设计。我们可以提供的不同数据集支持对 免疫细胞的表型和功能，确定免疫反应和跟踪，以及监测 免疫治疗过程中的免疫细胞产物。

PROJECT SUMMARY: IMMUNE MONITORING & FLOW CYTOMETRY SHARED RESOURCE (IM&FCSR) The Immune Monitoring and Flow Cytometry Shared Resource (IM&FCSR) provides investigators with services and research expertise, supporting a wide range of laboratory and clinical studies in cancer immunology and immunotherapy, transplant biology, infectious disease, and vaccine development. The IM&FCSR produces custom-made tetramer complexes of specific peptide and major histocompatibility complex molecules as unique reagents for assessing antigen-specific T cells and isolating these T cells for therapy trials. Our flow cytometry service offers a wide range of instrumentation, from single-laser benchtop analyzers to complex multi-laser cell sorters. We provide protocol-specific, flow cytometric immune monitoring to support immunotherapy trials. We quantify cytokine proteins, as a measure of immune and other cellular function, at technology platforms of high multiplicity, single cell, and/or ultrasensitivity. We perform specific cellular and molecular assays of native and genetically engineered T cells for laboratory research studies and for monitoring clinical samples. Technical assistance is available for data evaluation and interpretation, as is assistance in optimal experimental design. The different dataset we can provide enable the characterization of immune cell’s phenotypes and functionality, determining immune responses and tracking, and monitoring of immune cell products during immunotherapies.