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Shared Resource: Immune Monitoring & Flow Cytometry

共享资源：免疫监测

基本信息

批准号：
10319031
负责人：
Jianhong Cao
金额：
$ 28.25万
依托单位：
FRED HUTCHINSON CANCER CENTER
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-01-01 至 2024-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10319031
关键词：
Adherence Advisory Committees Biological Assay Biology Cancer Center Support Grant Cell physiology Cells Clinical Clinical Immunology Clinical Research Clinical Trials Communicable Diseases Communities Complex Custom Data Data Set Development Equipment Evaluation Experimental Designs Faculty Feedback Flow Cytometry Flow Cytometry Shared Resource Funding Genetic Engineering Goals Group Meetings Immune Immune response Immunologic Monitoring Immunotherapy Individual Laboratory Research Laboratory Study Lasers Maintenance Major Histocompatibility Complex Malignant Neoplasms Manufacturer Name Measures Molecular Monitor Patients Peptides Personal Satisfaction Phenotype Procedures Proteins Protocols documentation Reagent Reproducibility Research Research Personnel Resource Sharing Sampling Services Shapes Staff Development Surveys T-Lymphocyte Technology Therapy trial Training Transplantation Universities Washington Work antigen-specific T cells cancer immunotherapy cytokine engineered T cells immunotherapy trials instrument instrumentation laser cell sorter meetings member ranpirnase research study skills symposium tumor immunology vaccine development

项目摘要

PROJECT SUMMARY: IMMUNE MONITORING & FLOW CYTOMETRY SHARED RESOURCE (IM&FCSR) The Immune Monitoring and Flow Cytometry Shared Resource (IM&FCSR) provides investigators with services and research expertise, supporting a wide range of laboratory and clinical studies in cancer immunology and immunotherapy, transplant biology, infectious disease, and vaccine development. The IM&FCSR produces custom-made tetramer complexes of specific peptide and major histocompatibility complex molecules as unique reagents for assessing antigen-specific T cells and isolating these T cells for therapy trials. Our flow cytometry service offers a wide range of instrumentation, from single-laser benchtop analyzers to complex multi-laser cell sorters. We provide protocol-specific, flow cytometric immune monitoring to support immunotherapy trials. We quantify cytokine proteins, as a measure of immune and other cellular function, at technology platforms of high multiplicity, single cell, and/or ultrasensitivity. We perform specific cellular and molecular assays of native and genetically engineered T cells for laboratory research studies and for monitoring clinical samples. Technical assistance is available for data evaluation and interpretation, as is assistance in optimal experimental design. The different dataset we can provide enable the characterization of immune cell’s phenotypes and functionality, determining immune responses and tracking, and monitoring of immune cell products during immunotherapies.

项目概要：免疫监测和流式细胞术共享资源（IM&FCSR）免疫监测和流式细胞仪共享资源（IM&FCSR）为研究者提供服务和研究专业知识，支持广泛的癌症实验室和临床研究免疫学和免疫疗法，移植生物学，传染病和疫苗开发。的 IM&FCSR生产定制的特定肽和主要组织相容性的四聚体复合物复合分子作为独特的试剂，用于评估抗原特异性T细胞并分离这些T细胞，治疗试验我们的流式细胞术服务提供广泛的仪器，从单激光台式分析仪到复杂的多激光细胞分选仪。我们提供方案特异性流式细胞免疫监测来支持免疫治疗试验我们定量细胞因子蛋白，作为免疫和其他细胞因子的量度。功能，在高多样性，单细胞和/或超灵敏度的技术平台。我们执行特定的用于实验室研究的天然和遗传工程化T细胞的细胞和分子测定，用于监测临床样本。在数据评价和解释方面可获得技术援助，协助优化实验设计。我们可以提供的不同数据集可以表征免疫细胞的表型和功能，确定免疫反应和跟踪，以及监测免疫治疗过程中的免疫细胞产物。