Shared Resource: Immune Monitoring

共享资源：免疫监测

• 批准号: 9001916
• 负责人: Jianhong Cao
• 金额: $ 17.39万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9001916
• 关键词: Antigens; Biological; Biological Assay; Cancer Center Support Grant; Cell physiology; Cellular Assay; Clinical; Clinical Research; Clinical Trials; Enzyme-Linked Immunosorbent Assay; Evaluation; Frequencies; Goals; Human; Hybrids; Immunologic Monitoring; Immunologics; Liquid substance; Major Histocompatibility Complex; Mediator of activation protein; Methodology; Microspheres; Molecular; Peptides; Production; Proteins; Reader; Reagent; Research; Research Personnel; Resource Sharing; Resources; Robotics; Scintillation Counter; Services; System; T-Cell Receptor; T-Lymphocyte; Therapeutic; Work; abstracting; anticancer research; enzyme linked immunospot assay; fast protein liquid chromatography; instrumentation; research study; response

Immune Monitoring Shared Resource Project Summary/Abstract The Immune Monitoring Shared Resource provides investigators with a broad range of services and expertise to perform human protein and other biological mediator analyses and to provide precise evaluation of immunologic responses for both research and clinical studies. Resource services include the production of peptide-major histocompatibility complex Class-I tetramers, reagents useful for accessing antigen-specific T cells; molecular assays for interrogating T cell receptor usage and frequency, cellular assays for determining cell function, and high throughput analytical services using ELISA assay and Luminex microbead methodology. Support is provided for both single experiments as well as large long-term clinical studies. The IMSR works closely with the Therapeutic Manufacturing Shared Resource to support clinical trials. Instrumentation includes two fast protein liquid chromatography (FPLC) systems, multi-mode hybrid reader, ELISPOT reader, enzyme-linked immunosorbent assay (ELISA) reader, xCELLigence System, luminometer, two quantitative PCR (qPCR) systems, a robotic liquid handling system, a Top-Count scintillation counter and two Luminex 200 systems.

免疫监测共享资源 项目总结/摘要 免疫监测共享资源为研究者提供广泛的服务和专业知识 进行人体蛋白质和其他生物介质分析，并提供精确的评估， 研究和临床研究的免疫应答。资源服务包括制作 肽-主要组织相容性复合物I类四聚体，用于获得抗原特异性T 细胞;询问T细胞受体使用和频率的分子测定，测定 细胞功能和使用ELISA测定和Luminex微珠方法的高通量分析服务。 为单个实验以及大型长期临床研究提供支持。IMSR工作 与治疗制造共享资源密切合作，以支持临床试验。 仪器包括两个快速蛋白质液相色谱（FPLC）系统，多模式混合阅读器， ELISPOT读数器，酶联免疫吸附测定（ELISA）读数器，xCELLigence系统，光度计， 两个定量PCR（qPCR）系统、一个机器人液体处理系统、一个Top-Count闪烁计数器和 两个Luminex 200系统