权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Dual CHIP Functions Control Tau Triage In Alzheimer's Disease

双芯片功能控制阿尔茨海默氏病的 Tau 分类

基本信息

批准号：
10319914
负责人：
Todd Jonathan Cohen
金额：
$ 61.97万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-03-01 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10319914
关键词：
Alzheimer&apos s Disease Alzheimer&apos s disease brain Alzheimer&apos s disease model Alzheimer&apos s disease patient Animal Model Behavioral Binding Biochemical Biological Assay Biophysics Brain Client Clinical Cognition Cognitive deficits Complex Data Disease Disease Progression Dissociation Event Genetic Genetic study Goals Heat Shock 70kD Protein Binding Protein Heat shock proteins Heat-Shock Response Human Immunotherapy Impaired cognition Impairment Individual Light Link MAPT gene Mediating Mediator of activation protein Microtubule Stabilization Microtubules Modeling Modification Molecular Molecular Chaperones Mus Nerve Degeneration Neurodegenerative Disorders Neurofibrillary Tangles Neurons Onset of illness Pathogenesis Pathology Patients Play Process Property Protein Dephosphorylation Protein Microchips Proteins Quality Control Regulation Role Site Spinocerebellar Ataxias Tauopathies Testing Therapeutic Time Triage Ubiquitin Variant cognitive enhancement cognitive function factor C in vitro Assay in vivo innovation insight mouse model neuronal survival neuroprotection novel pre-clinical prevent protective factors protein expression proteostasis repaired response targeted treatment tau Proteins tau aggregation tau expression tau function tau mutation tau phosphorylation therapeutic target therapy development ubiquitin ligase ubiquitin-protein ligase

项目摘要

PROJECT SUMMARY/ABSTRACT The accumulation of aggregated tau protein in the brain is a defining feature of Alzheimer’s disease (AD) and a logical therapeutic target to prevent AD progression. Genetic evidence in mice strongly supports the notion that tau promotes cognitive deficits in AD. While anti-aggregation and immunotherapy approaches have emerged as potential strategies to reduce tau aggregation in the brain, these target late-stage tau intermediates. Much less is known about the early-stage events that give rise to tau aggregates in otherwise healthy neurons, a time period in which chaperone-dependent refolding acts in a compensatory manner to restore tau function as a critical microtubule (MT) stabilizing factor. We seek to define these early-stage tau triage decisions since clinically targeting tau in this earlier window is highly desirable to prevent tau accumulation, particularly in asymptomatic individuals years to decades from disease onset. The C-terminus of heat shock 70-interacting protein (or CHIP) plays a central role in orchestrating protein quality control. While most prior studies have pointed to CHIP’s E3 ligase activity as a principal mediator of client substrate degradation including tau, studies now indicate that CHIP contains a poorly understood intrinsic chaperone function that operates completely independent of its E3 ligase activity. Genetic studies of spinocerebellar ataxia 16 (SCAR 16) patients, a rare neurodegenerative disorder, showed that loss of CHIP co-chaperone activity alone is sufficient to cause neurodegeneration and cognitive dysfunction. These prior studies, combined with our compelling new preliminary data showing that CHIP directly binds and chaperones tau to facilitate its dephosphorylation, provide strong support for a new model of CHIP-mediated tau triage. We hypothesize that CHIP prevents aberrant tau modifications and aggregation, restores normal tau function and MT stabilization, and ameliorates AD-related cognitive decline. In Aim-1, we will dissect CHIP’s dual functions as a regulator of tau function and phosphorylation using biochemical and biophysical assays in vitro. These studies will provide new mechanistic insights into how CHIP targets and repairs abnormal tau species. In Aim-2, we will explore a novel role for CHIP in mediating neuroprotection via the stabilization of MTs. We will test the requirements for CHIP co-chaperone activity in maintaining MT integrity and hence promoting survival in neurons that would otherwise undergo degeneration. Finally, in Aim-3, we will test the clinical implications of CHIP co-chaperone function in restoring cognition in an animal model of AD. Overall, our proposal is significant because it will illuminate the early-stage events that determine how tau is initially processed and triaged. It is also innovative because it is the first to highlight dual molecular functions of CHIP that are relevant to tau pathogenesis and the progression of this devastating disease.

项目总结/文摘