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Mayo Clinic Physical Activity Research Center: Metabolomics and Proteomics Analysis Site

梅奥诊所体育活动研究中心：代谢组学和蛋白质组学分析网站

基本信息

批准号：
10320026
负责人：
IAN R LANZA
金额：
$ 152万
依托单位：
MAYO CLINIC ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-12-08 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10320026
关键词：
Adipose tissue Amino Acids Animals Bioinformatics Biological Biology Blood Brain Cerebrospinal Fluid Chemicals Clinic Clinical Collaborations Communities Core Facility Custom Data Dimensions Ensure Event Exercise Fatty Acids Funding GDF8 gene Genes Glucose Goals Human Individual Infrastructure Institution Isotopes Kidney Kinetics Knowledge Kynurenine Label Laboratories Leadership Lipids Liquid substance Liver Maps Mass Fragmentography Mass Spectrum Analysis Measurement Measures Metabolic Metabolism Mission Modification Molecular Molecular Profiling Muscle Muscle Proteins NMR Spectroscopy Outcome Oxidative Stress Peptide Synthesis Peptides Physical activity Plasma Positioning Attribute Post-Translational Protein Processing Process Protein Biosynthesis Proteins Proteome Proteomics Recording of previous events Reporting Research Research Activity Research Personnel Resolution Resources Role Saliva Sampling Services Shotguns Signal Transduction Site Skeletal Muscle Stable Isotope Labeling Statistical Methods System Techniques Tissue Sample Tissues Transcript Transducers Translations United States National Institutes of Health Universities Urine Validation Water Wisconsin base concept mapping design exosome experience experimental study in vivo indexing innovation insight member metabolome metabolomics metabolomics resource method development novel organic acid phosphoproteomics programs protein degradation protein metabolism response stable isotope

项目摘要

PROJECT SUMMARY This proposal unites complementary analytical expertise of Mayo Clinic and University of Wisconsin in pursuit of the goal of charting a molecular map of physical activity (PA). The overall goal of the Mayo Clinic Center for Metabolomics and Proteomics (MCCMAPS) is to apply state-of-the-art metabolomics and proteomics approaches to the MoTrPAC samples. We propose a metabolomics workflow that begins with untargeted profiling to identify candidate metabolites. From there, annotated metabolites will be validated, and unknown metabolites will be identified through a combination of novel compound synthesis, MS/MS data and NMR spectroscopy structural elucidation. The important metabolites that emerge from untargeted profiling will guide subsequent targeted quantitative metabolite measurements in plasma and tissue samples. We propose a proteomics workflow that includes global quantitative proteomics in muscle, adipose, and exosomes and assessment of posttranslational modifications using phosphoproteomics, acetylproteomics, and indices of oxidative modifications. Global, discovery proteomics will guide targeted, quantitative proteomics of salient proteins in plasma (e.g., irisin, myostatin). Our peptide synthesis capabilities allows for custom synthesis of any stable isotope labeled peptides for standards for quantitation. In addition, we will measure stable isotope enrichment of labeled amino acids in plasma, muscle compartments, and individual skeletal muscle proteins thus offering an insight on how PA influences amino acid kinetics, protein metabolism, and translation of gene transcripts to individual proteins. Isotopic enrichment of lipids in plasma and tissues will enable measurements of fatty acid kinetics in response to PA. Advanced statistical methods will fuse proteomics and metabolomics data to generate molecular concept maps of PA. Here we propose to leverage a productive ongoing collaboration between metabolomics and proteomics experts at Mayo and University of Wisconsin as a single integrated analytical resource dedicated to uncovering molecular signatures of PA. Our goal is to not only contribute our analytical expertise, but also contribute our knowledge of muscle and adipose tissue biology to the Molecular Transducers of Physical Activity Consortium (MoTrPAC).

项目总结