Core B: Glyco-genomics and Bioinformatics

核心 B：糖基因组学和生物信息学

• 批准号: 10321578
• 负责人: Hartmut Karl-Heinz Weiler
• 金额: $ 23.27万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10321578
• 关键词: Back; Biochemical; Bioinformatics; Biological; Biological Models; Bone Marrow; Cells; Clinical; Collaborations; Consult; Consultations; Cryopreservation; Data; Data Analyses; Data Set; Data Storage and Retrieval; Environment; Enzymes; Gene Expression; Gene Expression Profiling; Gene set enrichment analysis; Generations; Genomics; Glycobiology; Glycosaminoglycans; Goals; Hematopoietic; Laboratories; Laboratory Procedures; Libraries; Maintenance; Methods; Microfluidics; Modeling; Molecular; Polysaccharides; Population; Process; Proteoglycan; Protocols documentation; Quality Control; RNA; Resources; Sampling; Secure; Site; Statistical Data Interpretation; Structure; Techniques; Tissue-Specific Gene Expression; Transcript; analytical tool; base; cDNA Library; cloud storage; cost; data sharing; data submission; experimental study; genomic platform; glycosylation; indexing; programs; public database; repository; response to injury; single cell analysis; single-cell RNA sequencing; user-friendly

Core B - Project Summary/Abstract This Program Project will investigate the mechanisms by which glycosylation and glycans may alter the function, maintenance, differentiation, and overall function of hematopoietic cells. The PPG's approach towards these questions is to find correlations between the glycosylation state of cells, their proteoglycan repertoire, their biological responses to injury, and their gene expression programs in model system with altered function of two key glycosylation enzymes, i.e. ST6Gal1 and β4Gal1T1. The Glycogenomics Core (Core B) will support this effort by characterizing the gene expression program in various models via sequencing of RNA. The three Specific Aims of this Core are (Aim 1) to generate and sequence a large number of cDNA libraries derived from bulk RNA samples isolated from a large pool of cells; (Aim 2) to generate and sequence cDNA libraries from single cells, and (Aim 3) to conduct the computational processing, initial analysis, and curation of sequencing data.

核心B -项目总结/摘要 本计划项目将研究糖基化和聚糖可能改变功能的机制， 维持、分化和造血细胞的整体功能。PPG对这些问题的态度 问题是要找到细胞的糖基化状态，它们的蛋白多糖库，它们的 损伤的生物学反应，以及它们在具有两个功能改变的模型系统中的基因表达程序， 关键糖基化酶，即ST 6 Gal 1和β 4Gal 1 T1。糖基因组学核心（核心B）将支持这一点 通过RNA测序来表征各种模型中的基因表达程序。三 本核心的具体目的是（目的1）产生和测序大量来自以下的cDNA文库： 从大量细胞中分离的大量RNA样品;（目的2）产生cDNA文库并对其进行测序， 单细胞，以及（目的3）进行计算处理，初步分析和测序的管理 数据