Core B: Glyco-genomics and Bioinformatics

核心 B：糖基因组学和生物信息学

• 批准号: 10088966
• 负责人: Hartmut Karl-Heinz Weiler
• 金额: $ 27.25万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2025-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10088966
• 关键词: Back; Biochemical; Bioinformatics; Biological; Biological Models; Bone Marrow; Cells; Clinical; Collaborations; Consult; Consultations; Cryopreservation; Data; Data Analyses; Data Set; Data Storage and Retrieval; Databases; Environment; Enzymes; Gene Expression; Gene Expression Profiling; Gene set enrichment analysis; Generations; Genomics; Glycobiology; Glycosaminoglycans; Goals; Hematopoietic; Laboratories; Laboratory Procedures; Libraries; Maintenance; Methods; Microfluidics; Modeling; Molecular; Polysaccharides; Population; Process; Proteoglycan; Protocols documentation; Quality Control; RNA; Resources; Sampling; Secure; Site; Statistical Data Interpretation; Structure; Techniques; Tissue-Specific Gene Expression; Transcript; analytical tool; base; cDNA Library; cloud storage; cost; data sharing; data submission; experimental study; genomic platform; glycosylation; indexing; programs; repository; response to injury; single cell analysis; single-cell RNA sequencing; user-friendly

Core B - Project Summary/Abstract This Program Project will investigate the mechanisms by which glycosylation and glycans may alter the function, maintenance, differentiation, and overall function of hematopoietic cells. The PPG's approach towards these questions is to find correlations between the glycosylation state of cells, their proteoglycan repertoire, their biological responses to injury, and their gene expression programs in model system with altered function of two key glycosylation enzymes, i.e. ST6Gal1 and β4Gal1T1. The Glycogenomics Core (Core B) will support this effort by characterizing the gene expression program in various models via sequencing of RNA. The three Specific Aims of this Core are (Aim 1) to generate and sequence a large number of cDNA libraries derived from bulk RNA samples isolated from a large pool of cells; (Aim 2) to generate and sequence cDNA libraries from single cells, and (Aim 3) to conduct the computational processing, initial analysis, and curation of sequencing data.

核心B--项目摘要/摘要 该计划项目将研究糖基化和多糖改变功能的机制， 造血细胞的维持、分化和整体功能。PPG对这些问题的态度 问题是找到细胞的糖基化状态、它们的蛋白多糖谱系、它们的 在两种功能改变的模型系统中对伤害的生物反应及其基因表达程序 关键的糖基化酶，即ST6Gal1和β4Gal1T1。糖基因组学核心(核心B)将支持这一点 通过RNA测序确定不同模型中基因表达程序的特征。三位一体 本核心的具体目标是(目标1)生成和测序来自于 从大量细胞中分离的大量RNA样本；(目标2)从以下来源生成和测序 单个细胞，以及(目标3)进行测序的计算处理、初步分析和整理 数据。