Core C: Animal Models Core

核心 C：动物模型核心

• 批准号: 10379434
• 负责人: Hartmut Karl-Heinz Weiler
• 金额: $ 14.81万
• 依托单位: VERSITI WISCONSIN, INC.
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-15 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10379434
• 关键词: Affect; Animal Model; Animals; Biological; Biology; Blood; Communities; Consultations; Core Facility; Cryopreservation; Evaluation; Experimental Designs; Flowcharts; Generations; Genetic; Genomic Segment; Genotype; Joints; Methods; Modeling; Mouse Strains; Mus; Phenotype; Production; Rat Strains; Rattus; Reagent; Research; Research Institute; Research Personnel; Technical Expertise; Transgenic Mice; Transgenic Organisms; Validation; Variant; Wisconsin; clinically relevant; genetic information; in vivo; in vivo Model; medical schools; model design; novel; off-target mutation; preservation; programs; von Willebrand Disease

PROJECT SUMMARY: CORE C The Animal Model Core will support the generation of genetically modified mouse and rat models for the in vivo analysis of newly discovered variants of VWF. The Core will function in consultation with the PPG Investigators to develop the appropriate experimental design and methods to generate these animal models, produce and validate the required experimental reagents, utilize the existing facilities and technical expertise of the joint Transgenic Core Facility of the Blood Research Institute (BRI) and the Medical College of Wisconsin (MCW) to produce the desired animal, and manage the distribution of the genetically altered mice or rats to PPG Investigators and the wider research community.

项目概要：核心C 动物模型核心将支持生成用于体内研究的转基因小鼠和大鼠模型。 分析新发现的VWF变体。核心将与PPG研究者协商 开发适当的实验设计和方法来生成这些动物模型， 验证所需的实验试剂，利用现有的设施和联合技术专长， 血液研究所（BRI）和威斯康星州医学院（MCW）的转基因核心设施， 生产所需的动物，并管理遗传改变的小鼠或大鼠到PPG的分布 研究人员和更广泛的研究社区。