Immunogenics and Flow Cytometry

免疫原学和流式细胞术

• 批准号: 10320889
• 负责人: Benjamin G Vincent
• 金额: $ 31.47万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10320889
• 关键词: Animal Cancer Model; B cell repertoire; B-cell receptor repertoire sequencing; Big Data; Blood; Blood Cells; Budgets; Cancer Center Support Grant; Cells; Clinical; Clinical Investigator; Clinical Research; Collaborations; Communities; Complement; Computer software; Coupled; Cytokine Network Pathway; Cytometry; DNA sequencing; Data; Data Analyses; Data Set; Doctor of Philosophy; Elements; Equipment; Evaluation; Exons; Experimental Designs; Faculty; Flow Cytometry; Flow Cytometry Shared Resource; Genomics; Goals; Haplotypes; Human; Hybrids; Immune; Immune response; Immunogenomics; Immunologic Monitoring; Infrastructure; Investments; Knowledge; Malignant Neoplasms; Mass Spectrum Analysis; Methodology; Mission; Modeling; Mus; Pathway Analysis; Patients; Phenotype; Plasma; Recording of previous events; Reporting; Reproducibility; Research; Research Design; Research Personnel; Resource Sharing; Running; Sampling; Science; Services; Sorting - Cell Movement; Source; Students; System; T-Lymphocyte; Techniques; Technology; Time; Tissues; Training; Tumor-infiltrating immune cells; Work; anticancer research; computing resources; cost; data acquisition; exome; experience; experimental study; genetic signature; immunogenic; immunotherapy trials; in vivo; innovative technologies; interest; member; neoantigens; new technology; next generation sequencing; peer; pre-clinical; programs; response; single cell analysis; single-cell RNA sequencing; tumor

ABSTRACT: IMMUNOGEONOMICS AND FLOW CYTOMETRY SHARED RESOURCE The Immunogenomics and Flow Cytometry SR (IMCF) is a hybrid core that provides access to state-of-the-art techniques such as mass cytometry and immunogenomics analysis of bulk and single cell next generation sequencing data coupled with a high utilization Flow Cytometry facility with a 30-year LCCC history. New immune monitoring capabilities were developed in response to investigator needs, particularly for the analysis of immune responses in preclinical tumor models and human clinical research. The immunogenomics component offers a full suite of immunogenomics techniques, including immune gene signature and pathway analysis, cell phenotype deconvolution, MHC haplotype prediction, neoantigen prediction, T cell and B cell repertoire profiling, and systems level analyses such as cytokine network analysis and integrated survival modeling. This is joined by an investment in CyTOF for expanded panel analysis of cellular phenotypes. This component currently supports correlative science for tissues being collected from eight immunotherapy trials and multiple in vivo animal models of cancer. In addition to these new capabilities, the facility also houses a full complement of highly used analytic flow cytometers and cell sorters for both mouse and human work, New purchases in addition the CyTOF are two new spectral analytic machines. Most important for the research community, all SR components have expert staff to support investigators in experimental design, sample processing, data acquisition, and downstream analysis. IMCF is led by Dr. Vincent, MD, an expert in immunogenomics and Dr. Fisher, PhD who has led the LCC Flow facility since 2010 and has over 30 years’ experience in flow cytometry. The budget requested ($211,126) is a small proportion of the total operating budget of $1.7M. Flow cytometry is a traditionally heavily used facility; 80 members were users in fiscal year 2019.

摘要：免疫基因组学与流式细胞术资源共享