Immunogenics and Flow Cytometry

摘要：免疫地理学与流式细胞术共享资源 免疫基因组学和流式细胞术SR(IMCF)是一个混合核心，它提供了访问最先进的 下一代块状和单细胞的质量细胞术和免疫组学分析等技术 测序数据与具有30年LCCC历史的高利用率流式细胞仪设备相结合。新的 免疫监测能力是根据调查人员的需求而开发的，特别是在分析方面 临床前肿瘤模型和人类临床研究中的免疫反应。免疫基因组学 Component提供全套免疫基因组学技术，包括免疫基因签名和途径 分析、细胞表型去卷积、MHC单倍型预测、新抗原预测、T细胞和B细胞 曲目分析，以及系统层面的分析，如细胞因子网络分析和综合生存 模特儿。此外，还投资了CyTOF，用于细胞表型的扩展小组分析。这 Component目前支持从八项免疫治疗试验中收集的组织的相关科学 以及多种体内癌症动物模型。除了这些新功能外，该设施还配备了完整的 补充用于老鼠和人类工作的高度使用的分析流式细胞仪和细胞分选器，新 此外，CyTOF还购买了两台新的光谱分析仪。对研究来说最重要的是 社区，所有SR组件都有专家工作人员来支持研究人员进行实验设计、样本 处理、数据采集和下游分析。IMCF由文森特博士领导，医学博士是 免疫基因组学和费舍尔博士，他自2010年以来一直领导LCC Flow设施，已有30多年的经验。 掌握流式细胞术的经验。请拨的预算(211 126美元)只占业务总额的一小部分 预算为170万美元。流式细胞仪是一种传统的频繁使用的设备；在本财年，有80名成员是用户 2019年。

ABSTRACT: IMMUNOGEONOMICS AND FLOW CYTOMETRY SHARED RESOURCE The Immunogenomics and Flow Cytometry SR (IMCF) is a hybrid core that provides access to state-of-the-art techniques such as mass cytometry and immunogenomics analysis of bulk and single cell next generation sequencing data coupled with a high utilization Flow Cytometry facility with a 30-year LCCC history. New immune monitoring capabilities were developed in response to investigator needs, particularly for the analysis of immune responses in preclinical tumor models and human clinical research. The immunogenomics component offers a full suite of immunogenomics techniques, including immune gene signature and pathway analysis, cell phenotype deconvolution, MHC haplotype prediction, neoantigen prediction, T cell and B cell repertoire profiling, and systems level analyses such as cytokine network analysis and integrated survival modeling. This is joined by an investment in CyTOF for expanded panel analysis of cellular phenotypes. This component currently supports correlative science for tissues being collected from eight immunotherapy trials and multiple in vivo animal models of cancer. In addition to these new capabilities, the facility also houses a full complement of highly used analytic flow cytometers and cell sorters for both mouse and human work, New purchases in addition the CyTOF are two new spectral analytic machines. Most important for the research community, all SR components have expert staff to support investigators in experimental design, sample processing, data acquisition, and downstream analysis. IMCF is led by Dr. Vincent, MD, an expert in immunogenomics and Dr. Fisher, PhD who has led the LCC Flow facility since 2010 and has over 30 years’ experience in flow cytometry. The budget requested ($211,126) is a small proportion of the total operating budget of $1.7M. Flow cytometry is a traditionally heavily used facility; 80 members were users in fiscal year 2019.