Immunogenics and Flow Cytometry

免疫原学和流式细胞术

• 批准号: 10089827
• 负责人: Benjamin G Vincent
• 金额: $ 31.47万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2025-11-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10089827
• 关键词: Animal Cancer Model; B cell repertoire; B-cell receptor repertoire sequencing; Big Data; Blood; Blood Cells; Budgets; Cancer Center Support Grant; Cells; Clinical; Clinical Investigator; Clinical Research; Collaborations; Communities; Complement; Computer software; Coupled; Cytokine Network Pathway; Cytometry; DNA sequencing; Data; Data Analyses; Data Set; Doctor of Philosophy; Elements; Equipment; Evaluation; Exons; Experimental Designs; Faculty; Flow Cytometry; Flow Cytometry Shared Resource; Genomics; Goals; Haplotypes; Human; Hybrids; Immune; Immune response; Immunogenomics; Immunologic Monitoring; Infrastructure; Investments; Knowledge; Malignant Neoplasms; Mass Spectrum Analysis; Methodology; Mission; Modeling; Mus; Pathway Analysis; Patients; Phenotype; Plasma; Recording of previous events; Reporting; Reproducibility; Research; Research Design; Research Personnel; Resource Sharing; Running; Sampling; Science; Services; Sorting - Cell Movement; Source; Students; System; T-Lymphocyte; Techniques; Technology; Time; Tissues; Training; Tumor-infiltrating immune cells; Work; anticancer research; computing resources; cost; data acquisition; exome; experience; experimental study; genetic signature; immunogenic; immunotherapy trials; in vivo; innovative technologies; interest; member; neoantigens; new technology; next generation sequencing; peer; pre-clinical; programs; response; single cell analysis; single-cell RNA sequencing; tumor

ABSTRACT: IMMUNOGEONOMICS AND FLOW CYTOMETRY SHARED RESOURCE The Immunogenomics and Flow Cytometry SR (IMCF) is a hybrid core that provides access to state-of-the-art techniques such as mass cytometry and immunogenomics analysis of bulk and single cell next generation sequencing data coupled with a high utilization Flow Cytometry facility with a 30-year LCCC history. New immune monitoring capabilities were developed in response to investigator needs, particularly for the analysis of immune responses in preclinical tumor models and human clinical research. The immunogenomics component offers a full suite of immunogenomics techniques, including immune gene signature and pathway analysis, cell phenotype deconvolution, MHC haplotype prediction, neoantigen prediction, T cell and B cell repertoire profiling, and systems level analyses such as cytokine network analysis and integrated survival modeling. This is joined by an investment in CyTOF for expanded panel analysis of cellular phenotypes. This component currently supports correlative science for tissues being collected from eight immunotherapy trials and multiple in vivo animal models of cancer. In addition to these new capabilities, the facility also houses a full complement of highly used analytic flow cytometers and cell sorters for both mouse and human work, New purchases in addition the CyTOF are two new spectral analytic machines. Most important for the research community, all SR components have expert staff to support investigators in experimental design, sample processing, data acquisition, and downstream analysis. IMCF is led by Dr. Vincent, MD, an expert in immunogenomics and Dr. Fisher, PhD who has led the LCC Flow facility since 2010 and has over 30 years’ experience in flow cytometry. The budget requested ($211,126) is a small proportion of the total operating budget of $1.7M. Flow cytometry is a traditionally heavily used facility; 80 members were users in fiscal year 2019.

摘要：免疫地理学和流式细胞术共享资源 免疫基因组学和流式细胞术SR（IMCF）是一种混合核心，可提供最先进的 下一代大细胞和单细胞的大量细胞计数和免疫基因组学分析等技术 测序数据加上具有30年LCCC历史的高利用率流式细胞仪设施。新 免疫监测能力的发展是为了满足研究者的需要，特别是分析 临床前肿瘤模型和人类临床研究中的免疫应答。免疫基因组学 组件提供了一整套免疫基因组学技术，包括免疫基因签名和途径 分析、细胞表型解卷积、MHC单体型预测、新抗原预测、T细胞和B细胞 库分析和系统水平分析，如细胞因子网络分析和整合存活 建模此外，还投资于CyTOF，用于细胞表型的扩展面板分析。这 该组件目前支持从八项免疫治疗试验中收集的组织的相关科学 和多种癌症的体内动物模型。除了这些新功能外，该设施还拥有一个完整的 补充高度使用的分析流式细胞仪和细胞分选仪，用于小鼠和人类工作，新 购买除了CyTOF是两个新的光谱分析机器。对研究来说最重要的 社区，所有SR组件都有专家工作人员，以支持研究人员在实验设计，样品 处理、数据采集和下游分析。IMCF由医学博士Vincent领导，他是 Fisher博士自2010年以来一直领导LCC Flow设施，拥有超过30年的 有流式细胞术经验。请拨的预算（211 126美元）只占业务费用总额的一小部分。 预算170万元。流式细胞仪是一种传统上使用量很大的设施;在本财政年度有80名成员是用户 2019.