Redox Molecular Signaling Core

项目摘要 COBRE氧化还原生物学和心血管疾病中心的目标是建立一个全国性的 公认的先进的研究和培训计划，在氧化还原生物调节心血管疾病， 促进初级教师科研项目的发展，实现重大科研项目的自主资助。 核心C的发展，氧化还原分子信号核心，将提供COBRE研究人员和 受训人员可使用现代设备检测和量化活性氧， 活性氮物质和活性硫物质。此外，该设施将能够分析氧化还原- 通过氧化还原蛋白质组学和代谢组学对蛋白质功能进行依赖性控制， 细胞培养缺氧研究，并为建立氧化还原信号的细胞培养模型提供专业知识。这 一系列的分析技术，以评估氧化还原信号将是无与伦比的一个单一的设施在路易斯安那州。 氧化还原分子信号传导核心的建立将通过形成两个不同的 分析氧化还原生物学子核心和分子信号子核心，每个子核心都建立在 路易斯安那州立大学健康什里夫波特目前提供的现有服务。分析氧化还原生物学的建立 子核心将通过为现有的高性能液体核心设备增加功能来实现 色谱法（HPLC）和质谱法（MS），目前与研究核心设施。的 额外的关键设备将使高质量，定量氧化还原测量在一个集中的设施 专业的技术人员。分子信号子核心将通过添加密钥 设备和专业知识的核心设施，可通过病理学系。这个核心将提供 一个集中的设施，以协助分子生物学和建立细胞培养模型的氧化还原 信号传导，并将提供重要的设备，用于模拟培养中的缺氧细胞损伤， 使用高通量自动毛细管电泳进行分析。Redox的教职员工 分子信号核心将与COBRE研究人员密切合作，优化实验设计， 进行分析测量，提供核心设备使用方面的培训，并协助收集数据 和解释。这些服务将为COBRE调查人员提供宝贵的好处， 同时加强当地氧化还原生物学研究的基础设施。

PROJECT ABSTRACT The goals of the COBRE Center for Redox Biology and Cardiovascular Disease are to establish a nationally recognized advanced research and training program in redox biology regulation of cardiovascular disease and to facilitate the development of junior faculty research programs to achieve major independent research funding. The development of Core C, the Redox Molecular Signaling Core, will provide COBRE investigators and trainees with access to modern equipment for the detection and quantification of reactive oxygen species, reactive nitrogen species, and reactive sulfur species. Additionally, this facility will enable the analysis of redox- dependent control of protein function through redox proteomics and metabolomics, provide a centralized facility for cell culture hypoxia studies, and provide expertise for establishing cell culture models of redox signaling. This range of analytical techniques to assess redox signaling will be unmatched for a single facility in Louisiana. Establishment of the Redox Molecular Signaling Core will be accomplished through the formation of two distinct sub-cores, the Analytical Redox Biology Sub-Core and the Molecular Signaling Sub-Core, each building on existing services currently offered at LSU Health Shreveport. Establishment of the Analytical Redox Biology Sub-Core will occur through adding functionality to existing core equipment for high performance liquid chromatography (HPLC) and mass spectrometry (MS) currently associated with the Research Core Facility. The additional of key equipment will enable high quality, quantitative redox measurements at a centralized facility with dedicated technical staff. The Molecular Signaling Sub-Core will be formed through the addition of key equipment and expertise to core facilities available through the Department of Pathology. This core will provide a centralized facility for assistance with molecular biology and for establishing cell culture models of redox signaling and will provide access to vital equipment for modeling hypoxic cell injury in culture and for protein analysis using high throughput, automated capillary electrophoresis. The faculty and staff of the Redox Molecular Signaling Core will work closely with the COBRE investigators to optimize experimental design, perform analytical measurements, provide training on the use of core equipment, and assist with data collection and interpretation. These services will provide an invaluable benefit to the COBRE investigators to advance their individual projects while enhancing the infrastructure of local redox-biology research.