权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Redox Molecular Signaling Core

氧化还原分子信号核心

基本信息

批准号：
10715405
负责人：
Anthony Wayne Orr
金额：
$ 40.11万
依托单位：
LOUISIANA STATE UNIV HSC SHREVEPORT
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-01 至 2028-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10715405
关键词：
Biology Blood Vessels Capillary Electrophoresis Cardiovascular Diseases Cardiovascular Models Cardiovascular system Cause of Death Cell Culture Techniques Cell Hypoxia Cell Line Cell model Cell physiology Cells Centers of Research Excellence Communities Core Facility Data Analyses Data Collection Dedications Detection Development Ensure Equipment Experimental Designs Faculty Funding Generations Goals High Pressure Liquid Chromatography Hydrogen Sulfide Hypoxia Incidence Individual Infrastructure Institution Louisiana Mass Spectrum Analysis Measurement Measures Messenger RNA Methods Modeling Modernization Molecular Molecular Biology Oxidation-Reduction Pathogenesis Pathology Phase Post-Translational Protein Processing Preparation Production Program Development Protein Analysis Proteomics Reactive Nitrogen Species Reactive Oxygen Species Regulation Research Research Personnel Resources Sampling Services Signal Transduction Sulfur Techniques Technology Tissues Training Training Programs Transfection Work cell injury detection method detector faculty research improved mass spectrometer metabolomics next generation sequencing novel operation programs protein function

项目摘要

PROJECT ABSTRACT The goals of the COBRE Center for Redox Biology and Cardiovascular Disease are to establish a nationally recognized advanced research and training program in redox biology regulation of cardiovascular disease and to facilitate the development of junior faculty research programs to achieve major independent research funding. The Redox Molecular Signaling Core (Core C) provides COBRE investigators and trainees with access to modern equipment for the detection and quantification of reactive oxygen species, reactive nitrogen species, and reactive sulfur species. Additionally, this facility enables the analysis of redox-dependent control of protein function through redox proteomics and metabolomics, provides a centralized facility for cell culture hypoxia studies, and provides expertise for establishing cell culture models of redox signaling. This range of analytical techniques to assess redox signaling will be unmatched for a single facility in Louisiana. The functions of the Redox Molecular Signaling Core are accomplished through two distinct sub-cores, the Analytical Redox Biology Sub-Core and the Molecular Signaling Sub-Core. The Analytical Redox Biology Sub-Core utilizes equipment for high performance liquid chromatography (HPLC), specialized chemiluminescent detectors, and mass spectrometry (MS) to provide high quality, quantitative redox measurements at a centralized facility with dedicated technical staff. During Phase I, the institution purchased an Orbitrap Exploris 480 mass spectrometer that significantly expanded our capability to perform proteomic analysis of cell and tissue function in models of redox signaling. During Phase II, we will expand the functionality of this core through the purchase of new equipment for reactive sulfur species quantification and additional mass spectrometry equipment to enhance sensitivity. The Molecular Signaling Sub-Core offers a centralized facility for providing assistance with molecular biology, for establishing cell culture models of redox signaling, and for affording access to vital equipment for modeling hypoxic cell injury in culture and for protein analysis using high throughput, automated capillary electrophoresis. In Phase II, we will mature and expand the Molecular Signaling Sub-Core by adding additional core services based on the needs of the current COBRE faculty, such as improved methods for transient transfection of primary cells and enhanced access to next generation sequencing technologies. The faculty and staff of the Redox Molecular Signaling Core work closely with the COBRE investigators to optimize experimental design, perform analytical measurements, provide training on the use of core equipment, and assist with data collection and interpretation. These services provide an invaluable benefit to the COBRE investigators to advance their individual projects while enhancing the infrastructure of local redox-biology research.

项目摘要科布雷氧化还原生物学和心血管疾病中心的目标是建立一个全国性的公认的心血管疾病氧化还原生物调节和高级研究和培训计划促进初级教员科研项目发展，实现重大自主科研经费。氧化还原分子信号核心(核心C)为Cobre研究人员和受训人员提供访问用于检测和量化活性氧物种、活性氮物种、和活性硫物种。此外，该设备还可以分析蛋白质的氧化还原控制。通过氧化还原蛋白质组和代谢组学发挥作用，为细胞培养缺氧提供集中化的设施研究并为建立氧化还原信号的细胞培养模型提供专业知识。这一范围的分析对于路易斯安那州的一家工厂来说，评估氧化还原信号的技术将是无与伦比的。的功能氧化还原分子信号核心通过两个不同的子核心来完成，即分析氧化还原生物学亚核和分子信令亚核。分析氧化还原生物子核利用设备进行高效液相色谱仪、专门的化学发光检测器和质谱仪光谱(MS)在集中式设施中提供高质量、定量的氧化还原测量，敬业的技术人员。在第一阶段，该机构购买了Orbitrap Exploris 480质谱仪这大大扩展了我们对细胞和组织功能进行蛋白质组学分析的能力氧化还原信号。在第二阶段，我们将通过购买新的用于活性硫物种定量的设备和额外的质谱学设备以增强敏感度。分子信令子核心提供了一个集中的设施，用于为分子信号提供帮助生物学，用于建立氧化还原信号的细胞培养模型，并提供访问关键设备利用高通量自动毛细管模拟培养中的缺氧细胞损伤和蛋白质分析电泳法。在第二阶段，我们将通过添加额外的基于当前Cobre教职员工需求的核心服务，如改进的瞬变方法原代细胞的转基因和更好地获得下一代测序技术。教职员工和 Redox分子信号核心的工作人员与Cobre研究人员密切合作，以优化实验设计、执行分析测量、提供核心设备使用方面的培训，并协助处理数据收集和解读。这些服务为Cobre调查人员提供了宝贵的好处推进各自的项目，同时加强当地氧化还原生物学研究的基础设施。