Evolution, Optimization, and Application of Genome Editing Technologies
Evolution, Optimization, and Application of Genome Editing Technologies
批准号:
10330689
负责人:
J. KEITH JOUNG
金额:
$77.35万
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
未结题
起止时间:
2016-06-14 至 2026-12-31
关键词:
AddressAdenineArchitectureBiomedical ResearchCellsClustered Regularly Interspaced Short Palindromic RepeatsCytosineDNA Double Strand BreakDNA RepairDeaminaseDetectionEngineeringEpigenetic ProcessEvolutionGene MutationGenerationsGenesGoalsGuide RNAHeritabilityHumanModalityMutationNucleotidesOrganismProcessRNA-Directed DNA PolymeraseSingle-Stranded DNASiteTechnologyTherapeuticbase editorbiological researchendonucleaseexperimental studygene productgenome editinghuman diseaseimprovedinsertion/deletion mutationinterestnext generationnucleaseprime editortechnology development
中文摘要
项目摘要/摘要
基因编辑技术能够有效地定向引入所需的感兴趣的基因改变
生物种类繁多,在生物研究和人类治疗方面有着广泛的应用。首先-
代基因编辑平台使用位点特异性内切酶(例如,RNA引导的CRISPR-CAS核酸酶)
诱导靶向DNA双链断裂,使用细胞DNA修复机制创造突变。
最近开发的基因编辑平台包括CRISPR胞嘧啶或腺嘌呤碱基编辑程序,它们
使用Cas9定向的单链DNA脱氨酶来诱导靶向的C-T或A-G改变,
和CRISPR指导的主要编辑(使用依赖于逆转录酶的过程,
可以引起任何核苷酸替换或小的插入突变)。虽然基因编辑技术
在过去的十年里,开发速度加快了,现有平台的许多重要限制还没有
有待解决,仍有机会创造多种新的有影响力的技术,由最近的
预付款。在本MIRA的下一个周期中,重点将放在四个广泛重要的挑战上:1)扩展
基因编辑离靶检测技术的灵敏度和适用范围;2)工程下一代总理
具有增强和优化的架构的编辑者;3)为目标用户创建新的表观遗传编辑平台
人类基因的可遗传激活;以及4)开发新的通用模式,将所有基因编辑交付给
人类细胞。
英文摘要
Project Summary/Abstract
Gene editing technologies enable the efficient targeted introduction of desired genetic alterations of interest in
a wide range of organisms and have broad applications for biological research and human therapeutics. First-
generation gene editing platforms use site-specific endonucleases (e.g., RNA-guided CRISPR-Cas nucleases)
to induce targeted DNA double-stranded breaks, using cellular DNA repair mechanisms to create mutations.
More recently developed gene editing platforms include the CRISPR cytosine or adenine base editors, which
use Cas9-directed single-stranded DNA deaminases to induce targeted C-to-T or A-to-G alterations,
respectively, and CRISPR-guided prime editors (which use a reverse transcriptase-dependent process that
can induce any nucleotide substitution or small indel mutations). Although gene editing technology
development has accelerated over the past decade, many important limitations of existing platforms have yet
to be addressed and opportunities remain to create multiple new impactful technologies enabled by recent
advances. For the next cycle of this MIRA, the focus will be on four broadly important Challenges: 1) expand
the sensitivities and scope of gene editor off-target detection technologies; 2) engineer next-generation prime
editors with enhanced and optimized architectures; 3) create new epigenetic editing platforms for targeted
heritable activation of human genes; and 4) develop new universal modalities for delivery of all gene editors to
human cells.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Core 2: GENETIC ENGINEERING CORE
-
批准号:10670740
-
项目类别:
-
资助金额:$37.91万
-
财政年份:2019
-
负责人:J. KEITH JOUNG
-
依托单位:
Core 2: GENETIC ENGINEERING CORE
-
批准号:10469360
-
项目类别:
-
资助金额:$37.91万
-
财政年份:2019
-
负责人:J. KEITH JOUNG
-
依托单位:
Core 2: GENETIC ENGINEERING CORE
-
批准号:10238048
-
项目类别:
-
资助金额:$37.91万
-
财政年份:2019
-
负责人:J. KEITH JOUNG
-
依托单位:
Evolution, Optimization, and Application of Genome Editing Technologies
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批准号:10541872
-
项目类别:
-
资助金额:$77.35万
-
财政年份:2016
-
负责人:J. KEITH JOUNG
-
依托单位:
Advancing stem cell technologies using engineered zinc finger proteins
-
批准号:7979352
-
项目类别:
-
资助金额:$88.5万
-
财政年份:2010
-
负责人:J. KEITH JOUNG
-
依托单位:
Advancing stem cell technologies using engineered zinc finger proteins
-
批准号:8518420
-
项目类别:
-
资助金额:$84.99万
-
财政年份:2010
-
负责人:J. KEITH JOUNG
-
依托单位:
Advancing stem cell technologies using engineered zinc finger proteins
-
批准号:8306207
-
项目类别:
-
资助金额:$87.62万
-
财政年份:2010
-
负责人:J. KEITH JOUNG
-
依托单位:
Advancing stem cell technologies using engineered zinc finger proteins
-
批准号:8703137
-
项目类别:
-
资助金额:$87.62万
-
财政年份:2010
-
负责人:J. KEITH JOUNG
-
依托单位:
Advancing stem cell technologies using engineered zinc finger proteins
-
批准号:8143426
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项目类别:
-
资助金额:$87.62万
-
财政年份:2010
-
负责人:J. KEITH JOUNG
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依托单位:
ADVANCING ZINC FINGER NUCLEASES FOR TARGETED GENOME MANIPULATION
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批准号:8303297
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项目类别:
-
资助金额:$56.36万
-
财政年份:2009
-
负责人:J. KEITH JOUNG
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依托单位:
ADVANCING GENE-EDITING NUCLEASES FOR TARGETED GENOME MANIPULATION
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批准号:9113587
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项目类别:
-
资助金额:$56.18万
-
财政年份:2009
-
负责人:J. KEITH JOUNG
-
依托单位:
ADVANCING ZINC FINGER NUCLEASES FOR TARGETED GENOME MANIPULATION
-
批准号:8114124
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项目类别:
-
资助金额:$56.36万
-
财政年份:2009
-
负责人:J. KEITH JOUNG
-
依托单位:
ADVANCING ZINC FINGER NUCLEASES FOR TARGETED GENOME MANIPULATION
-
批准号:7901661
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项目类别:
-
资助金额:$57.33万
-
财政年份:2009
-
负责人:J. KEITH JOUNG
-
依托单位:
ADVANCING GENE-EDITING NUCLEASES FOR TARGETED GENOME MANIPULATION
-
批准号:8548014
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项目类别:
-
资助金额:$56.18万
-
财政年份:2009
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负责人:J. KEITH JOUNG
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依托单位:
DNA-binding specificities of Cys2His2 zinc fingers
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批准号:7772289
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项目类别:
-
资助金额:$37.08万
-
财政年份:2007
-
负责人:J. KEITH JOUNG
-
依托单位:
DNA-binding specificities of Cys2His2 zinc fingers
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批准号:7263487
-
项目类别:
-
资助金额:$40.94万
-
财政年份:2007
-
负责人:J. KEITH JOUNG
-
依托单位:
DNA-binding specificities of Cys2His2 zinc fingers
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批准号:7364673
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项目类别:
-
资助金额:$37.29万
-
财政年份:2007
-
负责人:J. KEITH JOUNG
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依托单位:
Zinc Finger Protein-Protein Interactions
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批准号:7026436
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项目类别:
-
资助金额:$30.76万
-
财政年份:2005
-
负责人:J. KEITH JOUNG
-
依托单位:
Zinc Finger Protein-Protein Interactions
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批准号:7471666
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项目类别:
-
资助金额:$1.2万
-
财政年份:2005
-
负责人:J. KEITH JOUNG
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依托单位:
Zinc Finger Protein-Protein Interactions
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批准号:6851938
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项目类别:
-
资助金额:$31.5万
-
财政年份:2005
-
负责人:J. KEITH JOUNG
-
依托单位:
海外基金