State-dependent interaction of antiepileptic drugs with voltage-dependent sodium channels and differential regulation of excitatory and inhibitory central neurons

抗癫痫药物与电压依赖性钠通道的状态依赖性相互作用以及兴奋性和抑制性中枢神经元的差异调节

• 批准号: 10332723
• 负责人: BRUCE P BEAN
• 金额: $ 47.47万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-01 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10332723
• 关键词: Action Potentials; Acute; Address; Affinity; Antiepileptic Agents; Axon; Binding; Brain; Bulla; Cannabidiol; Carbamazepine; Clinical; Closure by clamp; Data; Dependence; Drug Interactions; Drug Targeting; Experimental Designs; Fire - disasters; Frequencies; Glutamates; Goals; Interneurons; Kinetics; Laboratories; Lead; Lidocaine; Membrane; Neurons; Pathologic; Pattern; Pharmaceutical Preparations; Phenytoin; Physiological; Preparation; Property; Regulation; Research; Rest; Seizures; Slice; Sodium; Sodium Channel; Techniques; Testing; Work; biophysical techniques; drug action; excitatory neuron; experimental study; follow-up; hippocampal pyramidal neuron; inhibitory neuron; lamotrigine; novel; voltage; voltage clamp

The goal of the proposed research is to take a biophysical approach to understand how antiepileptic drugs targeted to voltage-dependent sodium channels regulate neuronal firing by differentially binding to different gating states of the channels. The work brings together two lines of research in the laboratory, one characterizing the state-dependent interaction of drugs like lidocaine, phenytoin, carbamazepine, and lacosamide with sodium channels and the other exploring how gating of sodium channels regulates firing of a variety of mammalian central neurons. A key property of antiepileptic drugs is differential binding to different gating states of sodium channels, but how this changes firing of particular kinds of central neurons to control pathological neuronal activity is poorly understood. For example, higher affinity binding to open and inactivated states results in use-dependence, with increased inhibition as channels cycle through open and inactivated states during action potentials. Yet, this does not easily explain their clinical action, because use-dependence might predict more potent inhibition of GABAergic inhibitory neurons, which typically fire at high frequencies, than glutamatergic excitatory neurons, which typically fire more slowly. We will examine how antiepileptic drugs interact with the gating of neuronal sodium channels and explore how state-dependent binding and unbinding regulates the firing patterns of a variety of excitatory and inhibitory neurons. We will follow up preliminary data showing that carbamazepine, phenytoin, and lamotrigine are all more effective in inhibiting firing of slower-firing glutamatergic pyramidal neurons than fast-spiking GABAergic neurons. We will analyze how these drugs and others (including the new anti- epileptic cannabidiol and a novel, more potent carbamazepine derivative) interact with gating of both native and cloned sodium channels and how the resulting changes in sodium current modify the firing patterns of a variety of excitatory and inhibitory neurons in a manner depending on the repertoire of other channels. The experimental design will combine recordings of action potential firing with voltage-clamp analysis of the underlying sodium currents, using intact neurons in brain slice, acutely dissociated neurons, and heterologously expressed cloned channels. A key feature will be to study action potential firing, channel gating kinetics, and drug action at 37 °C.

拟议研究的目标是采取生物物理学的方法来了解抗癫痫是如何发生的