High Spatial and Temporal Resolution Imaging Core

项目摘要 本提案的目标是建立和支持高空间和时间分辨率 成像核心(HSTRI或核心C)设施，将提供一系列最先进的仪器，使 用荧光指示剂对细胞和组织样本进行药理或遗传标记的可视化。 在专家工作人员的协助和对这些工具的不受限制的访问下，我们预计核心C将大大 提高项目负责人在拨款申请方面的竞争力，并促进他们的认可和 在国家和国际两级对各自领域的贡献。这个设施是专门设计的 以满足我们项目负责人的需求，但也将增强生物医学研究的整体能力 内华达大学里诺分校和北卡罗来纳大学医学院。核心C将完成以下具体任务 Cobre第一阶段的目标：具体目标1：建立、维护和运营一个设施，提供 用于细胞研究的最新高空间和时间分辨率成像方法 在健康和疾病中的结构、信号通路和功能。显微镜方面的最新进展已经 生产了一代商用仪器，为生物医学研究人员提供了 以前所未有的空间和空间研究生物系统的结构和功能之间的关系 接近或低于衍射极限的时间分辨率。这一特定目标的目标是使高度的空间和 时间分辨率成像可作为Cobre调查人员的核心工具。全面的核心 将实施管理计划，以有效维护这一核心的基础设施并确定访问的优先顺序 向科布雷调查人员提供核心服务。具体目标2：建议和培训调查人员使用 新技术。对用户进行HSTRI核心设备和适当样品的操作培训 准备工作，以及教育他们了解这些设施提供的科学可能性，是至关重要的一部分 最大限度地提高核心对生物医学研究的影响。核心人员将提供适当的培训和 对用户的监督。具体目标3：发展用户基础和业务计划，以维持 从长远来看，这是核心。将制定计划，在项目结束期间和结束后继续向核心提供支助 资助期通过用户收费。HSTRI的核心服务也将以 在Cobre调查人员未预留的时段内进行充值。UNR Med和UNR Med的许多资金充足的实验室 联合国研究中心表示有兴趣将高分辨率成像方法纳入他们的研究和未来 批准申请。

Project Summary The goal of the present proposal is to establish and provide support for a High Spatial and Temporal Resolution Imaging Core (HSTRI or Core C) facility that will offer a wide array of state- of-the-art instruments enabling visualization of cells and tissues samples pharmacologically or genetically labeled with fluorescent indicators. With the assistance of expert staff and unrestricted access to these instruments, we expect Core C to greatly enhance the competitiveness of Project Leaders in grant applications, and promote their recognition and contributions to their respective fields at the national and international level. This facility is specifically designed to meet the needs of our Project Leaders, but will also enhance the overall capacity for biomedical research at the University of Nevada, Reno, and UNR School of Medicine. Core C will accomplish the following Specific Aims during Phase I of the COBRE: Specific Aim 1: To establish, maintain, and operate a facility providing state-of-the-art high spatial and temporal resolution imaging methodologies for studying cellular structure, signaling pathways, and function in health and disease. Recent advances in microscopy have produced a generation of commercially available instruments that provide biomedical researchers the ability to investigate the relationship between structure and function of biological systems with unprecedented spatial and temporal resolution, near or below the diffraction limit. The goal of this Specific Aim is to make high spatial and temporal resolution imaging available as a core facility to COBRE investigators. A comprehensive core management plan will be implemented to effectively maintain the infrastructure of this core and prioritize access of core services to COBRE investigators. Specific Aim 2: To advise and train investigators in the use of the new technologies. Training users in the operation of the HSTRI core equipment and appropriate sample preparation, as well as educating them about the scientific possibilities offered by these facilities, is a crucial part of maximizing the core's impact on biomedical research. Core personnel will provide appropriate training and supervision of users. Specific Aim 3: To develop a user base and an operational plan that will sustain the core in the long-term. Plans will be developed to sustain support for the Core during and after the end of the funded period through user fees. HSTRI core services will also be offered to non-COBRE investigators on a recharge basis during time slots not reserved by COBRE investigators. Many well-funded labs at UNR Med and UNR have expressed interest in incorporating high resolution imaging methods into their research and future grant applications.