High Spatial and Temporal Resolution Imaging Core

项目摘要 本提案的目标是建立一个高空间和时间分辨率， 成像核心（HSTRI或核心C）设施，将提供各种最先进的仪器， 用荧光指示剂标记或遗传标记的细胞和组织样品的可视化。 在专家工作人员的协助下，我们可以不受限制地使用这些工具，我们预计核心C将大大提高 提高项目负责人在申请资助方面的竞争力，并促进他们的认可， 在国家和国际一级为各自领域作出贡献。这个设施是专门设计的 以满足我们的项目领导人的需求，但也将提高生物医学研究的整体能力， 内华达州大学、里诺和UNR医学院。核心C将完成以下具体任务 COBRE第一阶段的目标：具体目标1：建立、维护和运营一个设施， 用于研究细胞的最先进的高空间和时间分辨率成像方法 结构，信号通路，以及健康和疾病的功能。显微镜的最新进展 生产了一代商用仪器，使生物医学研究人员能够 研究生物系统的结构和功能之间的关系， 时间分辨率，接近或低于衍射极限。这个具体目标的目标是使高空间和 时间分辨率成像可作为COBRE研究人员的核心设施。全面的核心 将实施一项管理计划，以有效维护这一核心的基础设施，并优先考虑进入 COBRE调查员的核心服务具体目标2：向调查人员提供使用 新技术培训用户操作HSTRI核心设备和适当的样品 准备工作以及教育他们了解这些设施所提供的科学可能性是至关重要的一部分 最大化核心对生物医学研究的影响。核心人员将提供适当的培训， 用户的监督。具体目标3：建立一个用户基础和一项业务计划， 核心在长期。将制定计划，在2010年期间和之后继续支持核心小组。 通过用户付费资助期间。HSTRI核心服务也将提供给非COBRE调查员， 在COBRE调查人员未预留的时间段内充电。UNR Med的许多资金充足的实验室， UNR表示有兴趣将高分辨率成像方法纳入他们的研究和未来 补助金申请。

Project Summary The goal of the present proposal is to establish and provide support for a High Spatial and Temporal Resolution Imaging Core (HSTRI or Core C) facility that will offer a wide array of state- of-the-art instruments enabling visualization of cells and tissues samples pharmacologically or genetically labeled with fluorescent indicators. With the assistance of expert staff and unrestricted access to these instruments, we expect Core C to greatly enhance the competitiveness of Project Leaders in grant applications, and promote their recognition and contributions to their respective fields at the national and international level. This facility is specifically designed to meet the needs of our Project Leaders, but will also enhance the overall capacity for biomedical research at the University of Nevada, Reno, and UNR School of Medicine. Core C will accomplish the following Specific Aims during Phase I of the COBRE: Specific Aim 1: To establish, maintain, and operate a facility providing state-of-the-art high spatial and temporal resolution imaging methodologies for studying cellular structure, signaling pathways, and function in health and disease. Recent advances in microscopy have produced a generation of commercially available instruments that provide biomedical researchers the ability to investigate the relationship between structure and function of biological systems with unprecedented spatial and temporal resolution, near or below the diffraction limit. The goal of this Specific Aim is to make high spatial and temporal resolution imaging available as a core facility to COBRE investigators. A comprehensive core management plan will be implemented to effectively maintain the infrastructure of this core and prioritize access of core services to COBRE investigators. Specific Aim 2: To advise and train investigators in the use of the new technologies. Training users in the operation of the HSTRI core equipment and appropriate sample preparation, as well as educating them about the scientific possibilities offered by these facilities, is a crucial part of maximizing the core's impact on biomedical research. Core personnel will provide appropriate training and supervision of users. Specific Aim 3: To develop a user base and an operational plan that will sustain the core in the long-term. Plans will be developed to sustain support for the Core during and after the end of the funded period through user fees. HSTRI core services will also be offered to non-COBRE investigators on a recharge basis during time slots not reserved by COBRE investigators. Many well-funded labs at UNR Med and UNR have expressed interest in incorporating high resolution imaging methods into their research and future grant applications.