High Spatial and Temporal Resolution Imaging Core

高时空分辨率成像核心

• 批准号: 10077906
• 负责人: Normand Leblanc
• 金额: $ 20.4万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10077906
• 关键词: 3-Dimensional; Applications Grants; Area; Biological Process; Biomedical Research; Cardiovascular system; Cells; Cellular Structures; Centers of Research Excellence; Core Facility; Disease; Equipment; Fees; Fluorescence Resonance Energy Transfer; Funding; Future; Generations; Goals; Health; Human Resources; Image; Individual; Infrastructure; International; Label; Laboratories; Logistics; Medical; Methodological Studies; Microscope; Microscopy; Molecular; Molecular Medicine; Nevada; Pharmacology; Phase; Preparation; Research; Research Personnel; Research Project Grants; Resolution; Sampling; Science; Services; Signal Pathway; Signal Transduction; Specialist; Structure; Supervision; System; Technology; Time; Tissue Sample; Training; Universities; Visualization; base; biological systems; design; experimental study; fluorescence microscope; high resolution imaging; imaging modality; instrument; instrumentation; interest; investigator training; medical schools; new technology; operation; temporal measurement; two-photon

Project Summary The goal of the present proposal is to establish and provide support for a High Spatial and Temporal Resolution Imaging Core (HSTRI or Core C) facility that will offer a wide array of state- of-the-art instruments enabling visualization of cells and tissues samples pharmacologically or genetically labeled with fluorescent indicators. With the assistance of expert staff and unrestricted access to these instruments, we expect Core C to greatly enhance the competitiveness of Project Leaders in grant applications, and promote their recognition and contributions to their respective fields at the national and international level. This facility is specifically designed to meet the needs of our Project Leaders, but will also enhance the overall capacity for biomedical research at the University of Nevada, Reno, and UNR School of Medicine. Core C will accomplish the following Specific Aims during Phase I of the COBRE: Specific Aim 1: To establish, maintain, and operate a facility providing state-of-the-art high spatial and temporal resolution imaging methodologies for studying cellular structure, signaling pathways, and function in health and disease. Recent advances in microscopy have produced a generation of commercially available instruments that provide biomedical researchers the ability to investigate the relationship between structure and function of biological systems with unprecedented spatial and temporal resolution, near or below the diffraction limit. The goal of this Specific Aim is to make high spatial and temporal resolution imaging available as a core facility to COBRE investigators. A comprehensive core management plan will be implemented to effectively maintain the infrastructure of this core and prioritize access of core services to COBRE investigators. Specific Aim 2: To advise and train investigators in the use of the new technologies. Training users in the operation of the HSTRI core equipment and appropriate sample preparation, as well as educating them about the scientific possibilities offered by these facilities, is a crucial part of maximizing the core's impact on biomedical research. Core personnel will provide appropriate training and supervision of users. Specific Aim 3: To develop a user base and an operational plan that will sustain the core in the long-term. Plans will be developed to sustain support for the Core during and after the end of the funded period through user fees. HSTRI core services will also be offered to non-COBRE investigators on a recharge basis during time slots not reserved by COBRE investigators. Many well-funded labs at UNR Med and UNR have expressed interest in incorporating high resolution imaging methods into their research and future grant applications.

项目总结