High Spatial and Temporal Resolution Imaging Core

高时空分辨率成像核心

• 批准号: 10558652
• 负责人: Normand Leblanc
• 金额: $ 23.34万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2024-03-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10558652
• 关键词: 3-Dimensional; Acceleration; Applications Grants; Area; Biological Process; Biomedical Research; Cardiovascular system; Cells; Cellular Structures; Centers of Research Excellence; Charge; Core Facility; Disease; Equipment; Fees; Fluorescence Resonance Energy Transfer; Funding; Future; Generations; Goals; Health; Human Resources; Image; Individual; Infrastructure; Institution; International; Label; Laboratories; Medical; Methodological Studies; Microscope; Microscopy; Molecular; Molecular Medicine; Nevada; Phase; Preparation; Research; Research Personnel; Research Project Grants; Sampling; Science; Services; Signal Pathway; Signal Transduction; Specialist; Structure; Supervision; System; Technology; Time; Tissue Sample; Training; Universities; Visualization; base; biological systems; design; experimental study; fluorescence microscope; high resolution imaging; imaging modality; instrument; instrumentation; interest; investigator training; medical schools; new technology; operation; pharmacologic; temporal measurement; two-photon; ultra high resolution

Project Summary The goal of the present proposal is to establish and provide support for a High Spatial and Temporal Resolution Imaging Core (HSTRI or Core C) facility that will offer a wide array of state- of-the-art instruments enabling visualization of cells and tissues samples pharmacologically or genetically labeled with fluorescent indicators. With the assistance of expert staff and unrestricted access to these instruments, we expect Core C to greatly enhance the competitiveness of Project Leaders in grant applications, and promote their recognition and contributions to their respective fields at the national and international level. This facility is specifically designed to meet the needs of our Project Leaders, but will also enhance the overall capacity for biomedical research at the University of Nevada, Reno, and UNR School of Medicine. Core C will accomplish the following Specific Aims during Phase I of the COBRE: Specific Aim 1: To establish, maintain, and operate a facility providing state-of-the-art high spatial and temporal resolution imaging methodologies for studying cellular structure, signaling pathways, and function in health and disease. Recent advances in microscopy have produced a generation of commercially available instruments that provide biomedical researchers the ability to investigate the relationship between structure and function of biological systems with unprecedented spatial and temporal resolution, near or below the diffraction limit. The goal of this Specific Aim is to make high spatial and temporal resolution imaging available as a core facility to COBRE investigators. A comprehensive core management plan will be implemented to effectively maintain the infrastructure of this core and prioritize access of core services to COBRE investigators. Specific Aim 2: To advise and train investigators in the use of the new technologies. Training users in the operation of the HSTRI core equipment and appropriate sample preparation, as well as educating them about the scientific possibilities offered by these facilities, is a crucial part of maximizing the core's impact on biomedical research. Core personnel will provide appropriate training and supervision of users. Specific Aim 3: To develop a user base and an operational plan that will sustain the core in the long-term. Plans will be developed to sustain support for the Core during and after the end of the funded period through user fees. HSTRI core services will also be offered to non-COBRE investigators on a recharge basis during time slots not reserved by COBRE investigators. Many well-funded labs at UNR Med and UNR have expressed interest in incorporating high resolution imaging methods into their research and future grant applications.

项目摘要 本提案的目的是建立和为高空间和时间分辨率提供支持 成像核心（HSTRI或CORE C）设施，将提供各种各样的状态仪器 细胞和组织样品的可视化在药理学或遗传标记的荧光指标上。 在专家工作人员和对这些乐器的无限制访问的协助下，我们希望Core C极大 增强项目负责人在赠款申请中的竞争力，并促进其认可和 在国家和国际一级对各自领域的贡献。该设施是专门设计的 为了满足我们项目领导者的需求，但也将提高生物医学研究的总体能力 内华达大学里诺大学和UNR医学院。核心C将完成以下特定 在山脉第一阶段的目标：特定目标1：建立，维护和运营提供的设施 用于研究细胞的最先进的高空间和时间分辨率成像方法 结构，信号通路以及健康和疾病的功能。显微镜的最新进展 生产了一代商用仪器，可为生物医学研究人员提供能力 研究生物系统与前所未有的空间和功能之间的关系 时间分辨率，接近或低于衍射极限。这个特定目的的目的是使高空间和 时间分辨率成像可作为毛绒研究人员的核心设施。全面的核心 将实施管理计划以有效维护此核心的基础架构并确定访问的优先级 核心调查员的核心服务。特定目的2：向调查人员提供建议和培训 新技术。培训用户在HSTRI核心设备和适当样品的运行中进行培训 准备工作以及对这些设施提供的科学可能性进行教育是至关重要的部分 最大化核心对生物医学研究的影响。核心人员将提供适当的培训， 对用户的监督。特定目标3：制定用户群和一个将维持的运营计划 从长远来看。将制定计划，以维持在核心期间和之后的核心支持 通过用户费用资助的期限。 HSTRI核心服务还将提供给非近企业的调查员 在时间插槽中，毛crese研究者保留的充电基础。 UNR Med和 UNR表示有兴趣将高分辨率成像方法纳入其研究和未来 赠款申请。