Flow Cytometry Shared Resource

流式细胞仪共享资源摘要 共济会癌症中心(MCC)流式细胞术共享资源(FCSR)的使命是提供 为MCC人员提供全面的最先进的流式细胞仪服务和培训 具有成本效益的方式。FCSR在共济会癌症研究大楼(MCRB)和 癌症和心血管研究大楼(CCRB)。几年前，FCSR是几个 明尼苏达大学学术健康中心(AHC)的独立流式细胞仪设施； 由单独的中心、部门或研究所管理。这些设施和AHC的董事 领导层决定将流式细胞仪设施合并为一个行政保护伞，并将 来自AHC的财政支持。这一新的大学流式细胞仪资源(UFCR)提供了一种经济 规模，更多的资金用于购买设备，以及AHC中心和研究所之间的支持统一 这需要流式细胞仪设备和专业知识。它还允许MCC研究人员不在MCRB或 CCRB可以使用FCSR以外的流式细胞仪设施，并且仍然可以获得MCC设备使用补贴。 就使用情况而言，FCSR是UFCR财团4个成员中最大的(约80% 计时)和仪器(10个流式细胞仪中的7个、3个细胞分选器中的2个和2个数据分析工作中的2个 站点)。FCSR日常运营总监是Paul Champoux先生；他由3名全职人员提供支持 人事、AHC管理和由5名成员组成的科学董事会。克里斯托弗·彭内尔博士担任 作为MCC的流式细胞术科学总监，我在该委员会任职。他监管着所有与癌症有关的事务 使用FCSR的项目，以及关于FCSR或UFCR其他3个成员中的任何一个的决定都需要Dr。 Pennell的同意；这确保了FCSR和UFCR作为一个整体，满足了MCC的流式细胞术需求 研究人员。FCSR定期与行业领先者合作测试和采用新的流式细胞仪 技术，使其保持在流式细胞术的前沿。因此，FCSR已经有了仪器 在商业上市前采用新技术进行翻新。这些进步使我们受益 并为教师提供工具，以保持与同行的竞争力，所有这些都是以非常低的费用进行的。 未来5年的具体目标是：1)为MCC研究人员提供全天候访问高级流程的途径 2)协助MCC研究人员设计流式细胞仪实验、数据采集、 和数据解释；3)对MCC研究人员进行关于流式细胞术原理的培训，并培训用户 关于自助式分析仪；4)测试新试剂和设备并不断升级设备；以及5) 与MCC研究人员分享流式细胞术的前沿进展。

Flow Cytometry Shared Resource Summary The mission of the Masonic Cancer Center (MCC) Flow Cytometry Shared Resource (FCSR) is to provide comprehensive state-of-the-art services and training in all aspects of flow cytometry to MCC personnel in a cost-effective manner. The FCSR has facilities in both the Masonic Cancer Research Building (MCRB) and the Cancer and Cardiovascular Research Building (CCRB). Several years ago, the FCSR was one of several independent flow cytometry facilities in the Academic Health Center (AHC) at the University of Minnesota; each was administered by a separate Center, Department, or Institute. The directors of these facilities and AHC leadership decided to merge the flow cytometry facilities under one administrative umbrella with centralized financial support from the AHC. This new University Flow Cytometry Resource (UFCR) provided an economy of scale, more funds to purchase equipment, and uniformity of support among the AHC centers and institutes that require flow cytometry equipment and expertise. It also allowed MCC researchers not housed in MCRB or CCRB to access flow cytometry facilities other than FCSR and still receive an MCC subsidy for equipment use. The FCSR is the largest of the 4 members in the UFCR consortium in terms of usage (approximately 80% of billed hours) and instrumentation (7 of 10 flow cytometers, 2 of 3 cell sorters, and 2 of 2 data analysis work stations). The FCSR Director of Daily Operations is Mr. Paul Champoux; he is supported by 3 full-time personnel, AHC administration, and a 5-member Board of Scientific Directors. Dr. Christopher Pennell serves on this Board as the Scientific Director of Flow Cytometry for MCC. He has oversight for all cancer-related projects that use FCSR, and decisions regarding FCSR or any of the other 3 UFCR members requires Dr. Pennell's agreement; this ensures that FCSR, and UFCR as a whole, meet the flow cytometry needs of MCC researchers. The FCSR regularly partners with industry leaders to test and adopt new flow cytometry technologies, keeping it at the cutting-edge of flow cytometry. As a result, the FCSR has had instrumentation retrofitted with new technologies prior to commercial availability. These advancements have benefitted customers and provided tools for the faculty to remain competitive with their peers, all at very low expense. The specific aims for the next 5 years are to 1) provide MCC researchers with 24/7 access to advanced flow cytometric instruments; 2) aid MCC researchers in the design of flow cytometry experiments, data acquisition, and data interpretation; 3) educate MCC researchers regarding the principles of flow cytometry and train users on self-service analyzers; 4) beta-test new reagents and equipment and continually upgrade equipment; and 5) share cutting-edge advancements in flow cytometry with MCC researchers.