BAF - SR Component

生物分子分析公司-项目总结 生物分子分析设施（BAF）为各种服务提供了一个集中的环境， “组学”（基因组学、转录组学、蛋白质组学、脂质组学、代谢组学）和分子生物学的仪器 提供全面的生物信息学支持服务，如管道和数据分析 工具.该设施分为相互作用和相互支持的部分-蛋白质组学，基因组科学， 脂质组学/代谢组学、共享仪器和生物信息学。资源服务大学 弗吉尼亚州癌症中心（UVACC）成员通过提供实验设计，样品处理， 具有成本效益的数据收集、数据分析以及对手稿和赠款的支持。UVACC研究者 在当地获得各种各样的服务，这些服务可以决定 细胞水平，包括下一代测序，包括批量DNA/RNA-Seq，单细胞/细胞核RNA和 ATAC-seq，第三代长读测序，定量基因表达，基因分型，复杂蛋白 混合物，生物标志物分析，翻译后修饰，分子相互作用和动力学，脂质， 代谢物和药物分析。该设施的统一组织允许UVACC成员 在相同或相关的样品上进行许多这些实验。BAF的一个重点领域是 提供满足UVACC需求的先进仪器和实验。最近二 多年来，该设施在蛋白质组学和代谢组学领域增加了两台质谱仪， 能力NGS仪器。由于大多数BAF用户都是UVACC成员，因此与这些用户的互动 研究人员是获取新仪器和开发新技术的驱动力。的 UVACC执行委员会和研究核心管理办公室（ORCA -在医学院内 (SOM)院长办公室）密切合作，以促进这种环境。为此，SOM提供直接 每年向BAF提供预算支持（约占总预算的30%），与UVACC共同支付， UVACC成员以极具竞争力的价格提供高效的服务。

BIOMOLECULAR ANALYSIS FACILITY – PROJECT SUMMARY The Biomolecular Analysis Facility (BAF) provides a centralized setting for a diverse suite of services and instrumentation in the `omics' (genomics, transcriptomics, proteomics, lipidomics, metabolomics) and molecular interaction and provides comprehensive bioinformatics support services such as pipelines and data analysis tools. The facility is divided into sections that interact and support each other – Proteomics, Genomic Sciences, Lipidomics/Metabolomics, Shared Instrumentation, and Bioinformatics. The resource serves University of Virginia Cancer Center (UVACC) Members by providing expertise in experimental design, sample processing, cost-effective data collection, data analysis, and support for manuscripts and grants. UVACC investigators have access locally to a wide variety of services that can determine qualitative and quantitative changes at the cellular level, including Next Generation Sequencing, including bulk DNA/RNA-Seq, Single cell/nuclei RNA and ATAC-seq, third generation long-read sequencing, quantitative gene expression, genotyping, complex protein mixtures, biomarker profiling, post-translational modifications, molecular interactions and kinetics, lipids, metabolites, and drug analysis. The consolidated organization of the facility allows UVACC Members to perform many of these experiments on the same or related samples. One key area of focus within the BAF is providing access to advanced instrumentation and experiments that meet UVACC needs. Within the past two years, the facility added two mass spectrometers in the areas of proteomics and metabolomics and two higher capacity NGS instruments. As the majority of BAF users are UVACC Members, the interaction with these investigators is the driving force for acquiring new instrumentation and developing new techniques. The UVACC Executive Committee and Office of Research Core Administration (ORCA – within School of Medicine (SOM) Dean's Office) work closely together to foster this environment. To that end, the SOM provides direct budget support to the BAF every year (~30% total budget), which together with the UVACC co-pay, provides UVACC Members with highly effective services at remarkably competitive prices.