Functional analysis of autism risk genes during neural development using single cell seq
使用单细胞测序对神经发育过程中自闭症风险基因进行功能分析
基本信息
- 批准号:10334483
- 负责人:
- 金额:$ 56.75万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-03-15 至 2024-01-31
- 项目状态:已结题
- 来源:
- 关键词:ASH1L geneAddressAffectAutomobile DrivingBrainCNTNAP1 geneCell Culture TechniquesCell physiologyCellsChromatinChromatin Remodeling FactorClustered Regularly Interspaced Short Palindromic RepeatsCodeComplexComputing MethodologiesDevelopmentDiseaseEtiologyExhibitsGene ExpressionGenerationsGenesGeneticGenetic ModelsGenetic TranscriptionGenomicsGlutamatesGoalsHumanIndividualKnowledgeLifeMapsMethodsModelingModificationMolecularMutationNeuronsNeurophysiology - biologic functionOrganOrganoidsPathway interactionsRegulator GenesResearchResolutionRoleShapesSpecific qualifier valueSynaptic plasticitySystemTestingWorkZebrafishautism spectrum disorderbrain cellcell typecombinatorialdisorder riskestrogenicexperimental studygene regulatory networkgenome-widein vivoinnovationinsightloss of functionloss of function mutationmutantnerve stem cellneurodevelopmentneurogenesisneuromechanismnovelprogramsprotein protein interactionrelating to nervous systemresponserisk variantsingle cell sequencingsingle-cell RNA sequencingtranscription factortranscriptomicsvertebrate embryos
项目摘要
The brain represents a complex organ with a myriad of functionally diverse
cell types. Autism spectrum disorder (ASD) is thought to alter the combinatorial
transcriptional and post-transcriptional code responsible for specifying and
maintaining different cell functions in the vertebrate brain. The proposed studies
will test the central hypothesis that chromatin/transcriptional regulators identified
as ASD risk genes act as developmental switches during early
neurodevelopment, altering the gene expression network and subsequent cell
function.
In this proposal we combine single cell sequencing, with genetic, genomic
and computational approaches in zebrafish and embryoid bodies to i)
characterize the role of ASD-risk genes in healthy neural gene regulatory
networks (Aim 1); 2) Interrogate the effects of ASD loss-of-function (LOF)
mutations in different neural lineages (Aim 2). Cell types and developmental
lineages within the brain that are more significantly affected by each candidate
ASD risk gene will be identified using zebrafish and human cell–derived
organoids.
The experiments outlined in this proposal have the ultimate goal of
identifying how ADS risk genes affect neural gene expression and cell function
during vertebrate development.
大脑是一个复杂的器官,
细胞类型。自闭症谱系障碍(ASD)被认为是改变组合的
转录和转录后编码负责指定和
维持脊椎动物大脑中不同的细胞功能。拟议的研究
将测试中心假设,即染色质/转录调控鉴定
由于ASD风险基因在早期发育过程中起着发育开关的作用,
神经发育,改变基因表达网络和随后的细胞
功能
在这个提议中,我们将联合收割机单细胞测序与遗传、基因组
和计算方法在斑马鱼和胚状体i)
描述ASD风险基因在健康神经基因调控中的作用
网络(目标1); 2)询问ASD功能丧失(LOF)的影响
不同神经谱系中的突变(目标2)。细胞类型和发育
大脑中受每个候选人影响更大的谱系
ASD风险基因将使用斑马鱼和人类细胞衍生的
类有机体
该提案中概述的实验的最终目标是
确定ADS风险基因如何影响神经基因表达和细胞功能
在脊椎动物的发育过程中。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Antonio J Giraldez其他文献
Antonio J Giraldez的其他文献
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{{ truncateString('Antonio J Giraldez', 18)}}的其他基金
Deciphering the regulatory code that specifies different cell fates in development using single cell genomics
使用单细胞基因组学破译指定发育过程中不同细胞命运的监管代码
- 批准号:
10160929 - 财政年份:2020
- 资助金额:
$ 56.75万 - 项目类别:
Deciphering the regulatory code that specifies different cell fates in development using single cell genomics
使用单细胞基因组学破译指定发育过程中不同细胞命运的监管代码
- 批准号:
10579182 - 财政年份:2020
- 资助金额:
$ 56.75万 - 项目类别:
Deciphering the regulatory code that specifies different cell fates in development using single cell genomics
使用单细胞基因组学破译指定发育过程中不同细胞命运的监管代码
- 批准号:
9974094 - 财政年份:2020
- 资助金额:
$ 56.75万 - 项目类别:
Deciphering the regulatory code that specifies different cell fates in development using single cell genomics
使用单细胞基因组学破译指定发育过程中不同细胞命运的监管代码
- 批准号:
10362629 - 财政年份:2020
- 资助金额:
$ 56.75万 - 项目类别:
Functional analysis of autism risk genes during neural development using single cell seq
使用单细胞测序对神经发育过程中自闭症风险基因进行功能分析
- 批准号:
9893904 - 财政年份:2019
- 资助金额:
$ 56.75万 - 项目类别:
Functional analysis of autism risk genes during neural development using single cell seq
使用单细胞测序对神经发育过程中自闭症风险基因进行功能分析
- 批准号:
10558696 - 财政年份:2019
- 资助金额:
$ 56.75万 - 项目类别:
Functional analysis of autism risk genes during neural development using single cell seq
使用单细胞测序对神经发育过程中自闭症风险基因进行功能分析
- 批准号:
9765793 - 财政年份:2019
- 资助金额:
$ 56.75万 - 项目类别:
Functional analysis of autism risk genes during neural development using single cell seq
使用单细胞测序对神经发育过程中自闭症风险基因进行功能分析
- 批准号:
10087965 - 财政年份:2019
- 资助金额:
$ 56.75万 - 项目类别:
Molecular mechanisms of the maternal to zygotic transition
母体向合子转变的分子机制
- 批准号:
9277085 - 财政年份:2017
- 资助金额:
$ 56.75万 - 项目类别:
Molecular mechanisms of the maternal to zygotic transition
母体向合子转变的分子机制
- 批准号:
10685563 - 财政年份:2017
- 资助金额:
$ 56.75万 - 项目类别:
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