Molecular Mechanisms Integrating Circadian Timing and Photic Signaling
整合昼夜节律和光信号传导的分子机制
基本信息
- 批准号:10334518
- 负责人:
- 金额:$ 34.56万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-05-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAllelesAnimalsAutomobile DrivingBehaviorBehavioralBiological ProcessCell LineCellsCommunicationCouplingDataDependenceDiseaseDrosophila genusEnvironmentFeedbackGeneticGenetic TranscriptionHourImpairmentJet Lag SyndromeLengthLightLiteratureMediatingModelingMolecularMolecular GeneticsMotor ActivityNeuronsPacemakersPathway interactionsPeriodicityPhasePhenotypePhosphorylationPhosphotransferasesPhotoperiodPhotoreceptorsPhysiologic pulseProtein DephosphorylationPublishingRegulationResearchRoleSignal PathwaySignal TransductionSiteSleeplessnessSpeedSystemTestingTimeVisualWorkcell typecircadiancircadian pacemakerday lengthgene functiongenetic analysisgenetic manipulationgenetic resourceinsightintercellular communicationlight effectsmolecular clockmutantneural network architecturenonvisual photoreceptornovelphosphatase of regenerating liverrelating to nervous systemresponseshift work
项目摘要
Project Summary
Circadian clocks have evolved to appropriately align biological processes to the changing 24 h environment.
Genetic analyses of circadian locomotor activity rhythms in the fruit fly Drosophila have revealed transcriptional
feedback loops as the core organizing principle of circadian clocks. Yet the pace of these circadian feedback
loops is largely determined by protein phosphorylation and subsequent degradation, driving rhythmic
expression of clock components such as PERIOD (PER). In Drosophila, light is able to reset these oscillators
in part via degradation of the clock component TIMELESS (TIM). Remarkably, these clocks are highly
conserved among animals. Circadian clocks also enable the appropriate adaptation to seasonal changes in
day length or photoperiod. Yet while much is known about both core clock and photic input mechanisms, a
mechanistic understanding of how these two pathways collaborate to mediate responses light, including
changing photoperiod, is lacking in animals. Here a novel clock component has been discovered, the
phosphatase of regenerating liver-1 (PRL-1), that is also important for light mediated resetting and setting
behavioral phase under varying seasonal photoperiod. This research proposes to leverage the discovery of
PRL-1 to understand how the circadian clock integrates light information to drive appropriately timed behavior.
It will specifically address the neuronal basis of PRL-1 function including the role in specific photoreceptor
pathways, its function in autonomous and coupling neuronal oscillators, and the role of the light and clock
regulated clock component TIM in mediating PRL-1 effects. These studies exploit the discovery of a core clock
component with a novel role in photoperiod-dependent behavior. In addition, full advantage is taken of the
Drosophila system, including the conservation of the core clock machinery and clock neural network
architecture as well as extensive molecular genetic resources to examine gene function in the whole animal.
This research also leverages the ability to quantitatively examine molecular oscillations in FACS sorted and
intact neurons. This work could provide insights into how circadian clocks integrate environmental information
to yield timed behavior.
项目概要
生物钟已经发展到可以根据不断变化的 24 小时环境适当调整生物过程。
果蝇昼夜运动活动节律的遗传分析揭示了转录
反馈循环作为生物钟的核心组织原则。然而这些昼夜节律反馈的速度
环很大程度上由蛋白质磷酸化和随后的降解决定,驱动有节奏的
时钟组件的表达式,例如 PERIOD (PER)。在果蝇中,光能够重置这些振荡器
部分是由于时钟组件 TIMELESS (TIM) 的退化。值得注意的是,这些时钟非常
动物间保存。昼夜节律时钟还能够适当适应季节变化
日长或光周期。然而,尽管人们对核心时钟和光输入机制了解很多,但
对这两种途径如何协作介导光反应的机制理解,包括
改变光周期,是动物所缺乏的。这里发现了一种新颖的时钟组件,
再生肝磷酸酶 1 (PRL-1),对于光介导的重置和设置也很重要
不同季节光周期下的行为阶段。这项研究建议利用以下发现
PRL-1 了解生物钟如何整合光信息来驱动适当的定时行为。
它将专门探讨 PRL-1 功能的神经元基础,包括在特定光感受器中的作用
通路、其在自主和耦合神经元振荡器中的功能以及光和时钟的作用
调节时钟组件 TIM 调节 PRL-1 效应。这些研究利用了核心时钟的发现
在光周期依赖性行为中具有新作用的成分。此外,还充分利用了
果蝇系统,包括核心时钟机制和时钟神经网络的保护
结构以及广泛的分子遗传资源来检查整个动物的基因功能。
这项研究还利用了 FACS 中定量检查分子振荡的能力
完整的神经元。这项工作可以深入了解生物钟如何整合环境信息
产生定时行为。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Correction to: SleepMat: a new behavioral analysis software program for sleep and circadian rhythms.
更正:SleepMat:一种新的睡眠和昼夜节律行为分析软件程序。
- DOI:10.1093/sleep/zsae079
- 发表时间:2024
- 期刊:
- 影响因子:5.6
- 作者:
- 通讯作者:
SleepMat: a new behavioral analysis software program for sleep and circadian rhythms.
SleepMat:一种新的睡眠和昼夜节律行为分析软件程序。
- DOI:10.1093/sleep/zsac195
- 发表时间:2022
- 期刊:
- 影响因子:5.6
- 作者:Sisobhan,Shiju;Rosensweig,Clark;Lear,BridgetC;Allada,Ravi
- 通讯作者:Allada,Ravi
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{{ truncateString('Ravi Allada', 18)}}的其他基金
The Molecular and Cellular Basis of the Sleep Homeostat
睡眠稳态的分子和细胞基础
- 批准号:
10896547 - 财政年份:2023
- 资助金额:
$ 34.56万 - 项目类别:
The Molecular and Cellular Basis of the Sleep Homeostat
睡眠稳态的分子和细胞基础
- 批准号:
10665203 - 财政年份:2023
- 资助金额:
$ 34.56万 - 项目类别:
Molecular Mechanisms Integrating Circadian Timing and Photic Signaling
整合昼夜节律和光信号传导的分子机制
- 批准号:
10112971 - 财政年份:2018
- 资助金额:
$ 34.56万 - 项目类别:
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