Flow Cytometry Core

俄克拉荷马州医学研究基金会拥有运行流式细胞术核心超过 三十年。它在免疫学、细胞周期、心血管生物学领域提供了多名研究人员 和发育生物学，可使用使用流式分析和分离细胞所需的仪器 细胞计数术。 流式细胞术核心的目的是为该项目提供最先进的流式细胞术 CMRO COBRE 的领导者。这不仅包括获得所需的物理仪器，还包括 关于该技术的可能性和局限性的建议，并帮助设计和解释实验 使用这些方法。本 COBRE 中的项目将利用一些最常见的用途 这项技术，例如基于一个或多个细胞表面（或细胞内）表达的表型分析 使用荧光标记的抗体分析分子，并通过分选纯化这些群体。此外， 他们将使用流式细胞术对细胞进行功能评估，包括细胞周期分析、 钙信号传导以及线粒体体积和健康状况的评估。 一些更注重新陈代谢的检测，例如用于检测线粒体 Ca 2 积累的 Rhod2， 线粒体质量的测定和葡萄糖摄取测定并不是由我们的用户常规进行的，他们的 因此，实施将扩大该设施的专业知识。这将有助于实现 COBRE 的总体目标 扩大俄克拉荷马州的细胞代谢研究和专业知识。使用流式细胞术方法 分析细胞代谢变化是一个快速变化且相对新颖的领域，核心设施将不断发展 必要时为我们的研究人员提供推进研究所需的任何新工具 程序。

The Oklahoma Medical Research Foundation has had an operating Flow Cytometry Core for more than thirty years. It has provided multiple investigators in the areas of Immunology, Cell Cycle, Cardiovascular Biology and Developmental Biology with access to the instrumentation required to analyze and isolate cells using flow cytometry. The aim of the Flow Cytometry Core is to provide access to state-of-the-art flow cytometry to the Project Leaders in this CMRO COBRE. This includes not only access to the physical instruments required, but also advice on the possibilities and limitations of the technology and help designing and interpreting experiments that use these approaches. The projects in this COBRE will take advantage of some of the most common uses of this technology, such as phenotyping based upon the expression of one or more cell surface (or intracellular) molecules using fluorescently-labeled antibodies, and purification of these populations by sorting. In addition, they will use flow cytometry for the functional assessment of cells, including analysis of cell cycle, analysis of calcium signaling, and assessment of mitochondrial volume and health. Some of the more metabolism-focused assays such as Rhod2 to detect mitochondrial Ca+2 accumulation, determination of mitochondrial mass, and glucose uptake assays are not routinely done by our users, and their implementation will therefore broaden the expertise of the facility. This will help achieve the overall COBRE goal of expanding cellular metabolism research and expertise in Oklahoma. Use of flow cytometric approaches to analyze cellular metabolism changes is a fast-changing and relatively novel area, and the Core facility will evolve as necessary to provide our investigators with access to any new tools required to advance their research programs.