Flow Cytometry Core

俄克拉荷马医学研究基金会拥有一个运作中的流式细胞术核心已超过 三十年了。它在免疫学、细胞周期、心血管生物学等领域提供了多名研究人员 和发育生物学，可使用FLOW分析和分离细胞所需的仪器 细胞学。 流式细胞仪核心的目标是为该项目提供最先进的流式细胞仪 这个CMRO科布雷的领导者。这不仅包括获得所需的实物仪器，而且还包括 关于技术的可能性和局限性的建议，并帮助设计和解释实验 使用这些方法。此Cobre中的项目将利用一些最常见的 这种技术，例如根据一个或多个细胞表面(或细胞内)的表达进行表型鉴定 使用荧光标记抗体的分子，并通过分类纯化这些种群。此外, 他们将使用流式细胞术对细胞进行功能评估，包括分析细胞周期、分析 钙信号，以及线粒体体积和健康的评估。 一些更注重新陈代谢的分析方法，如Rhod2，用于检测线粒体钙积聚， 线粒体质量的测定和葡萄糖摄取分析不是由我们的用户常规进行的，他们的 因此，执行工作将扩大该机制的专门知识。这将有助于实现科布雷的总体目标 在俄克拉荷马州扩大细胞新陈代谢研究和专业知识。使用流式细胞术的方法 分析细胞代谢变化是一个快速变化和相对较新的领域，核心设施将不断发展 根据需要为我们的研究人员提供推进研究所需的任何新工具 程序。

The Oklahoma Medical Research Foundation has had an operating Flow Cytometry Core for more than thirty years. It has provided multiple investigators in the areas of Immunology, Cell Cycle, Cardiovascular Biology and Developmental Biology with access to the instrumentation required to analyze and isolate cells using flow cytometry. The aim of the Flow Cytometry Core is to provide access to state-of-the-art flow cytometry to the Project Leaders in this CMRO COBRE. This includes not only access to the physical instruments required, but also advice on the possibilities and limitations of the technology and help designing and interpreting experiments that use these approaches. The projects in this COBRE will take advantage of some of the most common uses of this technology, such as phenotyping based upon the expression of one or more cell surface (or intracellular) molecules using fluorescently-labeled antibodies, and purification of these populations by sorting. In addition, they will use flow cytometry for the functional assessment of cells, including analysis of cell cycle, analysis of calcium signaling, and assessment of mitochondrial volume and health. Some of the more metabolism-focused assays such as Rhod2 to detect mitochondrial Ca+2 accumulation, determination of mitochondrial mass, and glucose uptake assays are not routinely done by our users, and their implementation will therefore broaden the expertise of the facility. This will help achieve the overall COBRE goal of expanding cellular metabolism research and expertise in Oklahoma. Use of flow cytometric approaches to analyze cellular metabolism changes is a fast-changing and relatively novel area, and the Core facility will evolve as necessary to provide our investigators with access to any new tools required to advance their research programs.