Exploring the role of genomic repeats in cardiovascular disease heritability

探索基因组重复在心血管疾病遗传性中的作用

• 批准号: 10337196
• 负责人: Ronen Mukamel
• 金额: $ 15.66万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-01 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10337196
• 关键词: Affect; All of Us Research Program; Alleles; Atrial Fibrillation; Attention Deficit Disorder; Base Pairing; Base Sequence; Biological Assay; Blood; Blood Pressure; Cardiovascular Diseases; Cardiovascular system; Cataloging; Cause of Death; Cessation of life; Cholesterol; Computer software; Coronary Arteriosclerosis; DNA Microarray Chip; DRD4 gene; Data; Development; Diploidy; Disease; Family Study; Fragile X Syndrome; Future; Genes; Genetic; Genome; Genomics; Genotype; Goals; Health; Heritability; Human; Human Genome; Huntington Disease; Hypertension; Individual; Ischemic Stroke; Length; Light; Link; Lipids; Lipoprotein (a); Maps; Measures; Methodology; Methods; Minisatellite Repeats; Modeling; Mutation; Outcome; Phase; Phenotype; Play; Population; Procedures; Proteins; Repetitive Sequence; Research; Resolution; Role; Sample Size; Single Nucleotide Polymorphism; Statistical Algorithm; Statistical Methods; Stretching; Testing; Time; United States National Institutes of Health; Variant; Work; base; biobank; cardiovascular disorder risk; cardiovascular risk factor; cohort; genetic architecture; genetic association; genome sequencing; genome wide association study; genome-wide; genotyping technology; illness length; interest; open source; programs; rare variant; risk variant; trait; whole genome

PROJECT SUMMARY/ABSTRACT This project aims to explore the genetic component of cardiovascular diseases contributed by repeats in human genomes. Cardiovascular diseases, the leading cause of death in the US, have sizeable genetic components which remain unexplained by association studies. Recent studies of coronary artery disease, ischemic stroke, and atrial fibrillation explain less than a quarter of the heritability in these diseases observed in family studies. The gap between observed and explained heritability has persisted despite large increases in the sample sizes in genome-wide association studies. This ‘missing heritability’ hinders the understanding of the genetic basis for cardiovascular disease, and the development of genetically-informed therapies. A potential contributor to the missing heritability is structural variation in genomes, which is usually omitted from association studies. Genetic association studies typically focus on single nucleotide polymorphisms (SNPs)—i.e., single base pair changes—and do not account for structural variants—i.e., mutations affecting large stretches of the genome. Structural variants are difficult to resolve using short-read sequencing or array- based genotyping technologies. While structural variants are rarer than SNPs, they are responsible for more base pairs of variation per individual due to their large length. The proposed research program will quantify and characterize the cardiovascular impact of variable number tandem repeats (VNTRs), an understudied class of structural variants in which a specific nucleotide sequence is repeated a varying number of times in different individuals. The human genome contains thousands of VNTR regions, a few of which are already known to influence common diseases. The proposed research will leverage existing genotyped cohorts consisting of hundreds of thousands of individuals to conduct a systematic study of the role of VNTR length variation in cardiovascular diseases. These cohorts are genotyped using arrays which do not directly assay VNTR lengths. The PI will develop statistical methods to impute VNTR lengths into large cohorts, and characterize the contribution of VNTR variation to cardiovascular disease. Additionally, the PI will refine the genetic architecture of Lipoprotein(a), a protein encoded by a gene with a VNTR whose length is known to influence cardiovascular disease risk.

项目总结/文摘