Recognition and mechanism of N6-methyl adenosine modifications

N6-甲基腺苷修饰的识别和机制

• 批准号: 10339010
• 负责人: TAO PAN
• 金额: $ 4.83万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10339010
• 关键词: Address; Adenosine; Alternative Splicing; Biochemical; Biological Process; Cells; Exons; Gene Expression Regulation; Genetic Transcription; Human; Immune response; Laboratories; Messenger RNA; Modeling; Modification; Molecular; Nuclear; Play; Proteins; RNA; RNA Polymerase II; RNA Splicing; Reader; Regulation; Research; Role; Site; Structure; Testing; Translations; Untranslated RNA; epitranscriptomics; molecular modeling; transcriptome

Project Summary/Abstract N6-methyl-adenosine (m6A) is the most abundant internal modifications in messenger and long non-coding RNA. This modification occurs in many sites in mRNA with functions including splicing, export, localization, stability, translation, and immune response. Functional inquiries of the m6A modification have been intensely studied since 2011 and have become an integral component of the new field of epitranscriptomics. Studies from our laboratories and others indicate that m6A modifications exert their function through interactions with specific cellular proteins termed m6A readers. This proposal investigates the biological function of m6A reader proteins and addresses the underlying molecular and cellular mechanisms. Our proposed research will establish molecular models of m6A function in cells through two nuclear-localized m6A reader proteins and their mechanisms of action. Aim 1 will investigate the molecular and cellular mechanisms of co-transcriptional, m6A-dependent regulation of mRNA alternative splicing. We will test our model on HNRNPG/m6A-dependent control through RNA polymerase II pausing and the effect of nascent RNA structure using targeted approaches. Aim 2 will study the function and mechanism of HNRNPG assembly through a low-complexity region and the effect of assembly on interacting with m6A-modified RNA and alternative splicing. We will test a molecular model on these aspects using biochemical and cellular approaches. Aim 3 will study the m6A-dependent mechanism of two nuclear-localized m6A reader proteins that regulate transcriptome-wide alternative splicing. We will elucidate the interplay among these two proteins on specific exon targets and how this occurs.

项目总结/摘要 N6-甲基腺苷（m6 A）是信使和长非编码区中最丰富的内部修饰， 核糖核酸这种修饰发生在mRNA的许多位点，其功能包括剪接、输出、定位， 稳定性、翻译和免疫反应。对m6 a修改的功能调查已经被强烈地 自2011年开始研究，并已成为epitranscriptomics新领域的组成部分。研究 来自我们实验室和其他人的研究表明，m6 A修饰通过与 称为m6 A阅读器的特定细胞蛋白质。本研究旨在探讨m6 A阅读器的生物学功能 蛋白质，并解决了潜在的分子和细胞机制。我们的研究计划将 通过两种核定位m6 A阅读蛋白及其在细胞中功能建立m6 A分子模型 作用机制。 目的1研究m6 A依赖的共转录调控的分子和细胞机制 mRNA选择性剪接。我们将测试我们的模型对HNRNPG/m6 A依赖性控制通过RNA 聚合酶II暂停和新生RNA结构的影响，使用有针对性的方法。 目的二是通过低复杂性区域和高复杂性区域研究HNRNPG组装的功能和机制， 组装对与m6 A修饰的RNA相互作用和可变剪接的影响。我们将测试一种分子 使用生物化学和细胞方法对这些方面进行建模。 目的3：研究两种核定位m6 A阅读蛋白的m6 A依赖性机制， 全转录组可变剪接。我们将阐明这两种蛋白质之间的相互作用， 外显子靶点及其发生方式。