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Role of the MUC1/HIF-1a Complex in the kidney proximal tubule during ischemia-reperfusion injury.

MUC1/HIF-1a 复合物在缺血再灌注损伤期间肾近曲小管中的作用。

基本信息

批准号：
10349156
负责人：
Mohammad Al-bataineh
金额：
$ 11.82万
依托单位：
UNIVERSITY OF PITTSBURGH AT PITTSBURGH
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-12-15 至 2023-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10349156
关键词：
Acute Renal Failure with Renal Papillary Necrosis Adult Affect Apical Award Binding Biological Assay Cell Line Cell Nucleus Cell surface Cells Cessation of life Chimera organism Chronic Kidney Failure Clinical Co-Immunoprecipitations Complex Data Dimerization Distal Elements Epithelial Epithelial Cells Excess Mortality Financial Hardship Functional disorder Genes Genetic Genetic Transcription Glucose Glycoproteins Goals Grant Human Hypotension Hypoxia Immunofluorescence Microscopy In Vitro Incidence Injury Injury to Kidney Ischemia Kidney Knock-out Knockout Mice Lead Limb structure Location Mediator of activation protein Mercuric chloride Metabolic dysfunction Metabolism Morbidity - disease rate Morphology Mucin 1 protein Mus Names Nephrons Nuclear Oxygen Pathway interactions Patients Phosphorylation Play Proteins Proximal Kidney Tubules Publishing Pyruvate Kinase Recovery Regulation Renal function Reperfusion Injury Reporting Research Research Personnel Resistance Role Sepsis Severities Signal Transduction Surface Testing Therapeutic Intervention Thick Tissues Transactivation Transcriptional Activation Tubular formation Work base beta catenin career congenic experimental study hypoxia inducible factor 1 inhibitor ischemic injury kidney cell mortality mouse model mutant novel novel therapeutics pancreatic cancer cells prevent renal damage repaired response therapy design trafficking transcription factor urinary

项目摘要

Project Abstract. Acute kidney injury (AKI) is a common and devastating clinical problem. Despite the morbidity, mortality, and financial drain associated, there are no established therapies outside of dialytic treatment. Recent work has identified adaptive responses in the tubular epithelium to cope with ischemia-reperfusion injury (IRI). There is a critical need to identify how adaptive responses are regulated in AKI so that therapeutic interventions to prevent and treat AKI can be developed. Hypoxia inducible factor-1 (HIF-1) is a transcription factor regarded as the most significant mediator of cellular adaptive responses to hypoxic insult. There is emerging evidence that the transmembrane glycoprotein mucin 1 (MUC1) expressed on the apical surface of kidney epithelia plays a novel and important role in enhancing HIF-1 activity. We have strong preliminary data suggesting that genetic deletion of Muc1 from mouse tubular epithelial cells exacerbates damage especially in the proximal tubule (PT) from IRI, limits adaptive HIF-1 responses, alters tubular metabolism, and inhibits recovery. The central hypothesis of this application is that MUC1 protects the kidney during IRI by transactivation of the HIF-1 adaptive response specifically in the recovering proximal tubule. The specific aims are: 1. To determine if MUC1 protection of the kidney during IRI requires MUC1-dependent HIF-1a nuclear targeting and subsequently transcriptional activation of the HIF-1 pathway specifically in the recovering PT. I will test the hypothesis that MUC1 protects the kidney during IRI by transactivation of the HIF-1 adaptive response in the recovering PT. Experiments are proposed to stabilize HIF-1a in the proximal tubule in the Muc1 KO mice during IRI by introducing a PT KO of VHL, and treatment with vehicle or a specific MUC1 inhibitor to block MUC1 internalization and nuclear delivery. Results of these studies will reveal if MUC1 simply stabilizes HIF-1a levels, or if MUC1 nuclear targeting is essential for transduction of the protective HIF-1 pathway. 2. To characterize the MUC1-HIF-1a complex in cultured kidney cells. I will test the hypothesis that MUC1 stabilizes HIF-1a by direct binding. Experiments are proposed here to characterize the MUC1-HIF-1a complex using both in vitro pull-down assays and co-IP studies in primary PT cells and human kidney cell lines with wild type and mutant constructs, and to determine if MUC1 binding to HIF-1a results in its trafficking to the nucleus. The goal of my research is to fully characterize the MUC1-HIF-1a interaction and determine the mechanism of its protective role in the kidney PT during IRI. This information can be used to design therapies to induce MUC1 and limit the severity of AKI. This award will provide me with additional support to generate preliminary data for an R01-level grant to further investigate whether increased levels of renal MUC1 provide resistance to IRI.

项目摘要。急性肾损伤（AKI）是一种常见的、毁灭性的临床问题。尽管发病率很高，