Paper-based Breastmilk Collection System for Facile, In-Home Use

项目摘要/摘要 该项目将进行纸质设备的早期开发，以促进高效的 从母乳中回收高质量的核酸用于乳腺癌风险评估、检测 隐匿性疾病和流行病学研究，旨在揭示在疾病发展的最早事件 乳腺癌。收集母乳，其中含有数百万个乳腺上皮细胞 腺体是获取乳房组织的唯一非侵入性方法。目前的母乳采集方法 需要昂贵的冰上母乳隔夜运输，并极大地限制了使用这种重要的 生物菌素。拟议的装置将便于在一张小纸上收集和装载母乳 可以通过邮件发送并在室温下长期平放存储的卡片，或 无限期地，允许随后的核酸回收。因为标本可以由女性装载 在他们家的隐私中，由于该设备是纸质的，而且价格低廉，我们预计它将 促进从不同地理位置的大量女性的乳房细胞中收集核酸 地点。 虽然母乳采集设备的灵感来自采血卡，但它的设计是为了处理 大量的母乳，需要获得大量的核酸 癌症风险评估。因此，该设备将完成几个功能：操作 母乳通过毛细作用进入纸张，将多余的液体分离成一次性的一层 以及在主器件矩阵内保留富核部分。清除多余的液体 体积将加快捕获的流体的后续干燥，并在用户 在运输前不能将卡片彻底烘干。该装置还将加入防腐剂 放入样品中，以便于在室温下长期稳定。本节目的重点是 设计便于保留核酸和任何细胞(也含有核酸)的装置 在处理窗口中保持未溶解，同时将多余的液体从 设备的组件。由于我们的目标是基于纸张的矩阵，因此拟议的研究将集中在 评价母乳与不同孔径和表面化学成分的试纸的相互作用 没有添加防腐剂。这些研究将使不同的材料能够优先考虑 设备的组件、各层和其他设备特征的工作配置，以及 加速测试，以衡量极端运输和储存温度的影响。

Project Summary / Abstract This project will conduct early-stage development of a paper-based device to facilitate the efficient recovery of high-quality nucleic acids from breastmilk for use in breast cancer risk assessment, detection of occult disease, and epidemiologic studies designed to uncover the earliest events in the development of breast cancer. Collecting breastmilk, which contains millions of epithelial cells from the mammary gland, is the only noninvasive method of obtaining breast tissue. Current breastmilk collection methods require expensive overnight shipping of breastmilk on ice and greatly limit studies using this important biospecimen. The proposed device will facilitate the collection and loading of breastmilk on a small paper card that can be sent through the mail and stored flat at room temperature for long periods of time, or indefinitely, allowing subsequent recovery of nucleic acids. Because specimens can be loaded by women in the privacy of their homes, and because the device is paper-based and inexpensive, we anticipate it will facilitate collection of nucleic acids from the breast cells of large numbers of women in diverse geographic locations. While the breastmilk-collection device is inspired by blood-collection cards, it is designed to process substantially larger volumes of breastmilk, needed to obtain a large quantity of nucleic acids required for cancer risk assessment. The device therefore will accomplish several functions: the manipulation of breastmilk into the paper through capillary action, the separation of excess fluid into a disposable layer and the retention of a nucleic-rich fraction within the primary device matrix. Removal of the excess fluid volume will expedite subsequent drying of the captured fluid and simplify transport in the event the user cannot thoroughly dry the card before transport. The device will additionally incorporate preservatives into the specimen to facilitate long term stability at room temperature. This program focuses on the design of the device to facilitate retention of nucleic acids and any cells (also containing nucleic acids) remaining unlysed in the processing window, at the same time removing excess fluid from this component of the device. As we target a paper-based matrix, the proposed studies will focus on evaluating the interaction of breastmilk with papers of varied pore size and surface chemistry with and without added preservatives. These studies will enable the prioritization of materials for different components of the device, a working configuration for the layers and other device features, and accelerated testing to gauge the impact of extreme transport and storage temperatures.