Zebrafish Inner Ear Regeneration
斑马鱼内耳再生
基本信息
- 批准号:10349933
- 负责人:
- 金额:$ 22.32万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-01-01 至 2023-12-31
- 项目状态:已结题
- 来源:
- 关键词:Aminoglycoside AntibioticsAminoglycoside resistanceAnimalsBirdsBody SurfaceCapsaicinCell physiologyClustered Regularly Interspaced Short Palindromic RepeatsCommunitiesConsumptionDevelopmentEarExposure toFishesFluorescent in Situ HybridizationFutureGene ExpressionGene TargetingGeneticGenetic ModelsGoalsGrowthHairHair CellsHearing problemHourHumanInner Hair CellsLabyrinthLocationMammalsMapsMediatingMethodsModelingMolecular AnalysisNatural regenerationNuclearOrganPathway interactionsPharmaceutical PreparationsPredispositionProcessPropertyProteinsReagentRecoveryRegenerative capacityRegenerative researchReproducibilityResolutionSensory HairSeriesStudy modelsSupporting CellSystemTestingTimeToxic effectTransgenic OrganismsWorkZebrafishcell typeequilibration disorderexperimental studyfunctional restorationgenetic analysishair cell regenerationin vivoin vivo imaginglateral linemethod developmentpostnatal developmentrapid techniqueregenerativerestorationsingle cell analysissingle-cell RNA sequencingtooltranscriptome sequencingtranslational approachtranslational therapeuticswater flow
项目摘要
PROJECT SUMMARY
Mechanosensory hair cell loss in the inner ear is a leading cause of hearing and balance disorders.
Unfortunately hair cell loss is permanent as humans have little or no capacity for regenerative recovery of hair
cells. Although there has been recent progress in the restoration of hair cells in mammals by promoting
expression of genes and pathways used during development, functional restoration is largely incomplete and
within a limited window of postnatal development. By contrast non-mammalian species from fish to birds
readily replace hair cells from surrounding supporting cell precursors. Critical to understanding hair cell
regeneration is the identification of the subpopulations of supporting cells that sustain regeneration. The
zebrafish is well established as a system to study regeneration with the advantages of advanced genetics and
in vivo imaging of living animals. Considerable progress has been made on understanding regeneration in the
lateral line system, the series of mechanosensory hair cells on the surface of the body used detect water flow
that share many properties with hair cells of the inner ear. By contrast there have been very few studies of hair
cell regeneration in the zebrafish inner ear, mainly because there has been no reliable method for causing
rapid and reproducible damage. In addition little is known about supporting cell types in the zebrafish inner
ear. Overcoming both of these obstacles would establish the zebrafish inner ear as a new model for hair cell
regeneration. We propose to develop tools for rapid, reproducible damage and regeneration in the zebrafish
inner ear. We also propose to identify inner ear supporting cell types, including potential hair cell precursors.
Successful completion of these aims will provide important tools for the community to begin new studies in
zebrafish inner ear regeneration.
项目总结
内耳的机械感觉毛细胞丢失是导致听力和平衡障碍的主要原因。
不幸的是,毛细胞丢失是永久性的,因为人类很少或根本没有能力再生恢复头发。
细胞。尽管最近在恢复哺乳动物毛细胞方面取得了进展,这是通过促进
在发育、功能恢复过程中使用的基因和途径的表达在很大程度上是不完整的
在有限的后天发育时间内。相比之下,从鱼到鸟的非哺乳动物物种
容易地从周围的支持细胞前体中替换毛细胞。理解毛细胞的关键
再生是对维持再生的支持细胞亚群的鉴定。这个
斑马鱼作为一个研究再生的系统已经很好地建立了,它具有先进的遗传学和
活体动物的体内成像。在理解再生方面已经取得了相当大的进展
侧线系统,身体表面的一系列机械感应毛细胞用来检测水流
它与内耳的毛细胞有许多共同的特性。相比之下,对头发的研究很少。
斑马鱼内耳的细胞再生,主要是因为目前还没有可靠的方法来引起
快速和可重复性的损害。此外,对斑马鱼体内支持细胞类型的了解很少
耳朵。克服这两个障碍将使斑马鱼内耳成为毛细胞的新模型
再生。我们建议开发快速、可重复的斑马鱼损伤和再生工具
内耳。我们还建议确定内耳支持细胞的类型,包括潜在的毛细胞前体。
成功完成这些目标将为社区在以下方面开始新的研究提供重要工具
斑马鱼内耳再生。
项目成果
期刊论文数量(0)
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{{ truncateString('David W Raible', 18)}}的其他基金
Mitochondrial function and dysfunction in hair cells
毛细胞的线粒体功能和功能障碍
- 批准号:
9215463 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
Mitochondrial function and dysfunction in hair cells
毛细胞的线粒体功能和功能障碍
- 批准号:
10056217 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
CRISPR Screen for Hair Cell Development and Regeneration Regulators
毛细胞发育和再生调节因子的 CRISPR 筛选
- 批准号:
9186821 - 财政年份:2016
- 资助金额:
$ 22.32万 - 项目类别:
2013 Neural Crest & Cranial Placodes Gordon Research Conference
2013 神经嵴
- 批准号:
8594021 - 财政年份:2013
- 资助金额:
$ 22.32万 - 项目类别:
Drug Discovery for the Prevention of Hearing Loss
预防听力损失的药物发现
- 批准号:
8517843 - 财政年份:2011
- 资助金额:
$ 22.32万 - 项目类别:
Drug Discovery for the Prevention of Hearing Loss
预防听力损失的药物发现
- 批准号:
8877478 - 财政年份:2011
- 资助金额:
$ 22.32万 - 项目类别:
Drug Discovery for the Prevention of Hearing Loss
预防听力损失的药物发现
- 批准号:
8128231 - 财政年份:2011
- 资助金额:
$ 22.32万 - 项目类别:
Drug Discovery for the Prevention of Hearing Loss
预防听力损失的药物发现
- 批准号:
8287577 - 财政年份:2011
- 资助金额:
$ 22.32万 - 项目类别:
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