Administrative Supplement to Regulators of retinal metabolism in healthy and degenerating retinas

健康和退化视网膜视网膜代谢调节剂的行政补充

• 批准号: 10361928
• 负责人: John D Ash
• 金额: $ 13.05万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10361928
• 关键词: 5' -AMP-activated protein kinase; Adenosine Monophosphate; Administrative Supplement; Affect; Age related macular degeneration; Award; Bar Codes; Cell Count; Cell Death; Cell Survival; Cells; Chromium; Complementary DNA; Core Facility; DNA; Devices; Disease; Enzymes; Gene Expression; Genomics; Goals; Inherited; Libraries; Macular degeneration; Measurement; Measures; Metabolic; Metabolism; Microfluidic Microchips; Microfluidics; Mitochondria; Mutation; PPAR gamma; Parents; Pathway interactions; Pharmaceutical Preparations; Phosphotransferases; Photoreceptors; Procedures; Process; Production; Quality Control; RNA; Reagent; Research Infrastructure; Retina; Retinal Degeneration; Retinal Photoreceptors; Role; Sampling; Suspensions; Testing; Therapeutic; Time; Tissues; United States; age related; aqueous; cDNA Library; data quality; design; equipment acquisition; experience; gain of function; gene function; improved; inherited retinal degeneration; instrument; instrumentation; loss of function; mitochondrial dysfunction; oxidative damage; parent project; retinal neuron; single-cell RNA sequencing; success

Abstract: The retina hashigh metabolic activity,and retinal degenerations have been associated with mitochondrial dysfunction, dysregulation of metabolism, and toxic oxidative damage. However, little is known about how metabolism is maintained under normal conditions or is dysregulated in degenerating retinas. AMPK (AMP-activated protein kinase)is a key regulator of metabolism in highly metabolic tissues and is a candidate to regulate metabolism in photoreceptors, and its role in retinal metabolism will be rigorously studied in this proposed project using both gain-of- function and loss-of-function approaches. Peroxisome proliferator-activated receptor gamma coactivator-alpha (PGC-1α) and beta (PGC-1β) are key regulators of mitochondrialbiogenesis. Adenosine monophosphate dependent kinase (AMPK) is an important regulator of PGC-1 activity. Our goal in this study isto determine the rolesof AMPK and PGC-1activity in retinal photoreceptors.

摘要： 视网膜具有高代谢活性，视网膜变性与 线粒体功能障碍、代谢失调和毒性氧化损伤。然而，在这方面， 关于代谢在正常条件下是如何维持的或如何失调的，我们知之甚少。 退化的视网膜。AMPK（AMP活化蛋白激酶）是代谢的关键调节因子 在高度代谢的组织中，并且是调节光感受器中代谢的候选者， 在视网膜代谢中的作用将在这个拟议的项目中进行严格的研究， 功能和功能丧失方法。过氧化物酶增殖活化受体γ 辅激活因子-α（PGC-1α）和β（PGC-1β）是藻类生物发生的关键调节因子。 腺苷一磷酸依赖性激酶（AMPK）是PGC-1的重要调节因子 活动本研究的目的是确定AMPK和PGC-1活性在视网膜病变中的作用， 光感受器