Administrative Supplement to Regulators of retinal metabolism in healthy and degenerating retinas

健康和退化视网膜视网膜代谢调节剂的行政补充

基本信息

批准号：
10361928
负责人：
John D Ash
金额：
$ 13.05万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10361928
关键词：
5&apos -AMP-activated protein kinase Adenosine Monophosphate Administrative Supplement Affect Age related macular degeneration Award Bar Codes Cell Count Cell Death Cell Survival Cells Chromium Complementary DNA Core Facility DNA Devices Disease Enzymes Gene Expression Genomics Goals Inherited Libraries Macular degeneration Measurement Measures Metabolic Metabolism Microfluidic Microchips Microfluidics Mitochondria Mutation PPAR gamma Parents Pathway interactions Pharmaceutical Preparations Phosphotransferases Photoreceptors Procedures Process Production Quality Control RNA Reagent Research Infrastructure Retina Retinal Degeneration Retinal Photoreceptors Role Sampling Suspensions Testing Therapeutic Time Tissues United States age related aqueous cDNA Library data quality design equipment acquisition experience gain of function gene function improved inherited retinal degeneration instrument instrumentation loss of function mitochondrial dysfunction oxidative damage parent project retinal neuron single-cell RNA sequencing success

项目摘要

Abstract: The retina hashigh metabolic activity,and retinal degenerations have been associated with mitochondrial dysfunction, dysregulation of metabolism, and toxic oxidative damage. However, little is known about how metabolism is maintained under normal conditions or is dysregulated in degenerating retinas. AMPK (AMP-activated protein kinase)is a key regulator of metabolism in highly metabolic tissues and is a candidate to regulate metabolism in photoreceptors, and its role in retinal metabolism will be rigorously studied in this proposed project using both gain-of- function and loss-of-function approaches. Peroxisome proliferator-activated receptor gamma coactivator-alpha (PGC-1α) and beta (PGC-1β) are key regulators of mitochondrialbiogenesis. Adenosine monophosphate dependent kinase (AMPK) is an important regulator of PGC-1 activity. Our goal in this study isto determine the rolesof AMPK and PGC-1activity in retinal photoreceptors.

抽象的：视网膜的代谢活性和视网膜变性与线粒体功能障碍，代谢功能障碍和有毒的氧化物损伤。然而，关于如何在正常条件下保持新陈代谢或失调的新陈代谢知之甚少在退化视网膜中。 AMPK（AMP激活蛋白激酶）是代谢的关键调节剂在高度代谢组织中，是调节感光体代谢的候选者，其在这个拟议的项目中，在永久代谢中的作用将使用两者获得功能和功能丧失方法。过氧化物体增生剂激活受体伽马共激活因子-Alpha（PGC-1α）和β（PGC-1β）是线粒体生成的关键调节剂。腺苷一磷酸依赖性激酶（AMPK）是PGC-1的重要调节剂活动。我们在这项研究中的目标是确定其余的AMPK和PGC-1活动的作用感受器。