Transcriptional control of stress-induced resistance to retinal degeneration

摘要： 视网膜退行性变是一大群疾病，其特征是不可逆转地丧失 光感受器。这些细胞的死亡会导致永久性的视力丧失，从而使人虚弱。 对个人生活质量的影响。尽管视网膜退化的诱因各不相同，但 围绕光感受器死亡的机制通常是相似的，这表明有可能 基因/突变不依赖的方法，减少多种形式的视网膜变性的失明。我们 其他研究表明，STAT3在所有视网膜细胞中都被激活，包括光感受器和Müler细胞 在遗传性视网膜变性期间。进一步的研究表明，STAT3的激活起着至关重要的作用 在促进广泛的基因表达变化以提高细胞抵抗细胞死亡的能力方面发挥作用。 然而，尽管有这些令人印象深刻的发现，但在确定这些机制方面进展甚微。 其中STAT3规定了保护。在这个项目中，我们将使用最先进的技术，包括单细胞 Rna-seq，并将数据与细胞特异性芯片-seq整合，以全面鉴定所有基因和 视网膜Müler细胞和视杆细胞中受STAT3调控的转录网络。

Abstract: Retinal degenerations are a large cluster of diseases characterized by the irreversible loss of photoreceptors. The death of these cells results in a permanent loss of vision that can have debilitating impacts on an individual's quality of life. Despite the diversity among triggers for retinal degenerations, the mechanisms surrounding photoreceptor death are often similar, suggesting the possibility of developing gene/mutation-independent approaches to reduce blindness from multiple forms of retinal degeneration. We and others have shown that STAT3 is activated in all retinal cells, including photoreceptors and Müller cells during inherited retinal degeneration. Additional work has shown that activation of STAT3 plays an essential role in promoting a wide array of gene expression changes to increase the cell’s capacity to resist cell death. However, despite these impressive findings, little progress has been made in identifying the mechanisms by which STAT3 regulates protection. In this project, we will use state of the art techniques including single-cell RNA-seq and integrating the data with cell-specific ChIP-seq to comprehensively identify all genes and transcriptional networks regulated by STAT3 in retinal Müller cells and rods.