MOLECULAR BIOLOGY

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 分子生物学模块主要通过从组织中提纯DNA和基于聚合酶链式反应的基因分型，为视觉眼镜蛇的研究人员提供了支持。该模块还通过维护用于实时聚合酶链式反应和Western blots基因表达分析的设备来支持研究人员。阿什博士是这个单元的负责人，他监督着它的日常运作。凭借在实时聚合酶链式反应、蛋白质印迹分析、DNA纯化和聚合酶链式反应基因分型方面的丰富经验，阿什博士有资格监督这一模块。自1994年以来，Ash博士一直致力于转基因小鼠的研究。在这14年的时间里，Ash博士维持了大约30个转基因小鼠品系。他的实验室目前每年对5到10,000个样本进行基因分型。卡尔博士是助理主任。卡尔博士在动物基因分型以及实时聚合酶链式反应和酶联免疫吸附试验分析方面拥有丰富的经验。分子生物学模块的操作由一名全职研究助理法特梅·沙里亚蒂指导。项目由服务请求发起。服役结束后，Shariati女士完成了一份报告，说明工作的完成情况和所有结果。该报告将包含一张凝胶图片，记录准确的聚合酶链式反应反应，以及对阳性和阴性结果的评估。所有报告在退还给用户之前都要经过模块总监的批准。如果结果不令人满意，Shariati夫人会重复分析。该单元以每年约8,000次反应的速度运行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Module has supported investigators of the Vision COBRE in their research programs primarily through DNA purification from tissues and PCR-based genotyping. The module also supports investigators through maintenance of equipment used for gene expression analysis by real-time PCR, and Western blots. Dr. Ash is the director of this module and he oversees its day to day operation. Dr Ash is qualified to supervise this module based on his extensive experience in real-time PCR, Western blot analysis, DNA purification, and PCR genotyping. Dr Ash has worked with genetically modified mice since 1994. During this 14 year period, Dr Ash has maintained approximately 30 lines of genetically modified mice. His lab currently genotypes 5 to 10,000 samples per year. Dr. Carr is the assistant director. Dr Carr has extensive experience in animal genotyping, as well as analysis by Real-Time PCR and ELISA. Operations of the Molecular Biology Module are conduced by a full time research assistant Fatemeh Shariati. Projects are initiated by a service request. Following completion of service Mrs. Shariati completes a report describing the completion of the job and all results. The report will contain a gel picture documenting accurate PCR reactions, and an assessment of positive and negative results. All reports are approved by the module director before they are returned to the users. If the results are not satisfactory, Mrs. Shariati will repeat the analysis. The module is operating at a rate of approximately 8,000 reactions per year.