MOLECULAR BIOLOGY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 分子生物学模块主要通过组织DNA纯化和基于PCR的基因分型支持Vision COBRE的研究人员进行研究计划。该模块还通过维护用于实时PCR和Western印迹进行基因表达分析的设备来支持研究人员。 阿什博士是这个模块的主任，他监督其日常运作。 Ash博士在实时PCR、蛋白质印迹分析、DNA纯化和PCR基因分型方面具有丰富的经验，因此有资格监督该模块。 阿什博士自1994年以来一直从事转基因小鼠的研究。 在这14年的时间里，Ash博士饲养了大约30种转基因小鼠。他的实验室目前每年对5到10,000个样本进行基因分型。 卡尔医生是副主任。卡尔博士在动物基因分型以及实时PCR和ELISA分析方面拥有丰富的经验。分子生物学模块的操作由全职研究助理Fatemeh Shariati进行。 项目由服务请求启动。服务结束后，Shariati女士完成一份报告，说明工作完成情况和所有结果。 报告将包含记录准确PCR反应的凝胶图片，以及阳性和阴性结果的评估。所有报告在返回给用户之前都要经过模块主管的批准。 如果结果不令人满意，Shariati夫人将重复分析。 该模块以每年约8，000次反应的速度运行。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular Biology Module has supported investigators of the Vision COBRE in their research programs primarily through DNA purification from tissues and PCR-based genotyping. The module also supports investigators through maintenance of equipment used for gene expression analysis by real-time PCR, and Western blots. Dr. Ash is the director of this module and he oversees its day to day operation. Dr Ash is qualified to supervise this module based on his extensive experience in real-time PCR, Western blot analysis, DNA purification, and PCR genotyping. Dr Ash has worked with genetically modified mice since 1994. During this 14 year period, Dr Ash has maintained approximately 30 lines of genetically modified mice. His lab currently genotypes 5 to 10,000 samples per year. Dr. Carr is the assistant director. Dr Carr has extensive experience in animal genotyping, as well as analysis by Real-Time PCR and ELISA. Operations of the Molecular Biology Module are conduced by a full time research assistant Fatemeh Shariati. Projects are initiated by a service request. Following completion of service Mrs. Shariati completes a report describing the completion of the job and all results. The report will contain a gel picture documenting accurate PCR reactions, and an assessment of positive and negative results. All reports are approved by the module director before they are returned to the users. If the results are not satisfactory, Mrs. Shariati will repeat the analysis. The module is operating at a rate of approximately 8,000 reactions per year.