Core C Immunophenotyping

科布雷应用免疫学和病理过程中心的目标是建立一个全国性的 公认的免疫学和炎症方面的高级研究和培训计划，并促进发展 对初级教员的研究项目，实现重大自主研究经费。大容量多分析 流式细胞术和显微镜是免疫学研究的关键研究工具，然而， 高质量、可重现的数据需要在协议开发方面具有广泛的专业知识。的发展。 核心C，免疫表型核心，将通过提供COBRE作为共享资源实验室 研究人员获得技术专长，以利用现有最先进的免疫表型鉴定 这项技术目前在路易斯安那州立大学什里夫波特健康科学中心可用。核心C级人员 将在免疫表型鉴定领域的一系列实验技术方面拥有专业知识，如多分析物 细胞外和细胞内流式细胞术、多分析珠阵列、超分辨荧光免疫组织化学 和原位杂交。核心C人员将与Cobre调查人员密切合作， 优化实验设计，制定免疫表型方案，进行免疫表型实验， 并分析数据。因此，C芯将成为科布雷PI实验室和LSUHSC-S研究中心之间的通道 核心设施，允许这些实验室将他们的研究扩展到免疫学、炎症等领域 与这些实验室尝试自己进行这些技术相比，这些技术可以更快地传播和传播疾病。这个 核心C的开发将推进Cobre调查人员的个人项目，同时加强 本地免疫学和炎症研究，从而使整个机构受益。

The goals of the COBRE Center for Applied Immunology and Pathological Processes are to establish a nationally recognized advanced research and training program in immunology and inflammation and to facilitate the development of junior faculty research programs to achieve major independent research funding. High capacity multi-analytical flow cytometry and microscopy are critical research tools for immunological studies, however, generating high quality, reproducible data requires extensive expertise in protocol development. The development of Core C, the Immunophenotyping Core, will serve as a Shared Resource Laboratory by providing COBRE investigators access to technical expertise to take advantage of existing state-of-the-art immunophenotyping technology currently available at Louisiana State University Health Science Center-Shreveport. Core C personnel will have expertise in a range of experimental techniques in the field of immunophenotyping such as multianalyte extracellular and intracellular flow cytometry, multi-analyte bead arrays, super-resolution fluorescent immunohistochemistry and in situ hybridizations. Core C personnel will work closely with COBRE investigators to optimize experimental design, develop immunophenotyping protocol, execute immunophenotyping experiments, and analyze data. Thus, Core C will be a conduit between COBRE PI laboratories and the LSUHSC-S Research Core Facility, allowing these laboratories to extend their research into the fields of immunology, inflammation and infectious disease more quickly than if these labs attempted to perform these techniques themselves. The development of Core C will advance individual COBRE investigators projects while enhancing infrastructure of local immunology and inflammation research, thus benefiting the whole institution.