Core C Immunophenotyping

COBRE应用免疫学和病理过程中心的目标是建立一个全国性的 在免疫学和炎症方面公认的先进研究和培训计划，并促进发展 初级教师的研究计划，以实现重大的独立研究经费。高容量多分析 流式细胞术和显微镜是免疫学研究的重要研究工具，然而， 高质量、可重复的数据需要在方案制定方面的广泛专业知识。的发展 核心C，免疫表型核心，将作为一个共享资源实验室，提供COBRE 研究者获得技术专长，以利用现有最先进的免疫表型分析 该技术目前在路易斯安那州州立大学健康科学中心-什里夫波特可用。C类核心人员 将在免疫表型领域的一系列实验技术，如多分析物 细胞外和细胞内流式细胞术，多分析物微珠阵列，超分辨率荧光免疫组织化学 和原位杂交。核心C人员将与COBRE调查人员密切合作， 优化实验设计，制定免疫分型方案，执行免疫分型实验， 并分析数据。因此，核心C将成为COBRE PI实验室和LSUHSC-S研究之间的管道 核心设施，使这些实验室能够将其研究扩展到免疫学、炎症 和传染病的速度比这些实验室尝试自己执行这些技术更快。的 核心C的开发将推进COBRE研究人员的个人项目，同时增强 局部免疫学和炎症研究，从而使整个机构受益。

The goals of the COBRE Center for Applied Immunology and Pathological Processes are to establish a nationally recognized advanced research and training program in immunology and inflammation and to facilitate the development of junior faculty research programs to achieve major independent research funding. High capacity multi-analytical flow cytometry and microscopy are critical research tools for immunological studies, however, generating high quality, reproducible data requires extensive expertise in protocol development. The development of Core C, the Immunophenotyping Core, will serve as a Shared Resource Laboratory by providing COBRE investigators access to technical expertise to take advantage of existing state-of-the-art immunophenotyping technology currently available at Louisiana State University Health Science Center-Shreveport. Core C personnel will have expertise in a range of experimental techniques in the field of immunophenotyping such as multianalyte extracellular and intracellular flow cytometry, multi-analyte bead arrays, super-resolution fluorescent immunohistochemistry and in situ hybridizations. Core C personnel will work closely with COBRE investigators to optimize experimental design, develop immunophenotyping protocol, execute immunophenotyping experiments, and analyze data. Thus, Core C will be a conduit between COBRE PI laboratories and the LSUHSC-S Research Core Facility, allowing these laboratories to extend their research into the fields of immunology, inflammation and infectious disease more quickly than if these labs attempted to perform these techniques themselves. The development of Core C will advance individual COBRE investigators projects while enhancing infrastructure of local immunology and inflammation research, thus benefiting the whole institution.