Chemoenzymatic synthesis of bacterial nonulosonic acids and glycans

细菌非酮糖酸和聚糖的化学酶法合成

基本信息

  • 批准号:
    10364735
  • 负责人:
  • 金额:
    $ 30.78万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-03-05 至 2025-01-31
  • 项目状态:
    未结题

项目摘要

Project Summary Chemoenzymatic synthesis of bacterial nonulosonic acids and glycans Sialic acids are a family of wide-spread nonulosonic acids (alpha-keto acids with a nine-carbon backbone) in vertebrates and in some pathogenic bacteria. Bacteria also produce nonulosonic acids that have not been found in animals. These bacterium-specific nonulosonic acids have been used by bacteria as part of their capsular polysaccharides (CPSs) and lipopolysaccharides (LPSs) which are bacterial virulence factors and potential vaccine candidates. Structurally defined bacterial CPSs, LPSs, and the corresponding oligosaccharide repeating units are important probes to study their roles in bacterial infection and microbe-host interaction including their influence on host immune systems. These carbohydrates are attractive synthetic targets but pose significant synthetic challenges. In addition to bacterial specific nonulosonic acids, they may contain other monosaccharide building blocks that have not been found in mammals. The glycosidic linkages found in bacterial polysaccharides are also much more diverse than those in mammalian glycans. We propose to develop efficient chemoenzymatic methods to synthesize bacterial nonulosonic acids and their glycans as part of CPS and LPS oligosaccharides of pathogenic bacteria. These represent biologically important and synthetically challenging targets. In the current proposal duration, the focus will be on bacterial glycans containing legionaminic acid and their derivatives. Three specific aims are: 1, Synthesize acceptors and monosaccharide chemoenzymatic synthons for one-pot multienzyme (OPME) glycosylation systems; 2, Identify and characterize candidate sugar-1-P kinases, UDP-sugar synthetases, and glycosyltransferases; and 3, Chemoenzymatic synthesis of bacterial polysaccharide repeating units containing legionaminic acid or derivatives. The enzymes identified and the monosaccharides designed are important tools and reagents for accessing synthetic challenging carbohydrates and glycoconjugates that are not limited to the targets described in the proposal. The oligosaccharides produced are essential probes for better understanding the important roles of bacterial polysaccharides. They are also candidates for synthesizing structurally defined carbohydrate-protein conjugate vaccines to combat bacterial infections.
项目总结

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Xi Chen其他文献

Xi Chen的其他文献

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{{ truncateString('Xi Chen', 18)}}的其他基金

High-throughput closed-loop direct aberration sensing and correction for multiphoton imaging in live animals
用于活体动物多光子成像的高通量闭环直接像差传感和校正
  • 批准号:
    10572572
  • 财政年份:
    2023
  • 资助金额:
    $ 30.78万
  • 项目类别:
Crosstalk between the ER Stress Response and Mitochondrial Fatty Acid Oxidation in MYC-driven Breast Cancer
MYC 驱动的乳腺癌中 ER 应激反应与线粒体脂肪酸氧化之间的串扰
  • 批准号:
    10581179
  • 财政年份:
    2023
  • 资助金额:
    $ 30.78万
  • 项目类别:
Proteostasis Reprogramming in Mutant KRAS-Driven Cancers
突变 KRAS 驱动的癌症中的蛋白质稳态重编程
  • 批准号:
    10587281
  • 财政年份:
    2022
  • 资助金额:
    $ 30.78万
  • 项目类别:
A Life Course Approach to Understanding Racial and Ethnic Disparities in Alzheimer's Disease and Related Dementias and Health Care
了解阿尔茨海默病及相关痴呆症和医疗保健中种族和民族差异的生命全程方法
  • 批准号:
    10650381
  • 财政年份:
    2022
  • 资助金额:
    $ 30.78万
  • 项目类别:
A Life Course Approach to Understanding Racial and Ethnic Disparities in Alzheimer's Disease and Related Dementias and Health Care
了解阿尔茨海默病及相关痴呆症和医疗保健中的种族和民族差异的生命全程方法
  • 批准号:
    10448032
  • 财政年份:
    2022
  • 资助金额:
    $ 30.78万
  • 项目类别:
Chemoenzymatic construction of synthetic human milk oligosaccharide (HMO) glycome
合成人乳低聚糖 (HMO) 糖组的化学酶法构建
  • 批准号:
    10567752
  • 财政年份:
    2022
  • 资助金额:
    $ 30.78万
  • 项目类别:
Chemoenzymatic construction of synthetic human milk oligosaccharide (HMO) glycome
合成人乳低聚糖 (HMO) 糖组的化学酶法构建
  • 批准号:
    10710393
  • 财政年份:
    2022
  • 资助金额:
    $ 30.78万
  • 项目类别:
Elucidating perifoveal vascular development in infants
阐明婴儿中心凹周围血管发育
  • 批准号:
    10696178
  • 财政年份:
    2022
  • 资助金额:
    $ 30.78万
  • 项目类别:
Improving oral health awareness and dental referrals for adult patients receiving palliative care
提高接受姑息治疗的成年患者的口腔健康意识和牙科转诊
  • 批准号:
    10348739
  • 财政年份:
    2021
  • 资助金额:
    $ 30.78万
  • 项目类别:
Chemoenzymatic synthesis of bacterial nonulosonic acids and glycans
细菌非酮糖酸和聚糖的化学酶法合成
  • 批准号:
    10553186
  • 财政年份:
    2021
  • 资助金额:
    $ 30.78万
  • 项目类别:

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