Decoding the non-canonical polyubiquitin chains using chemical approaches
使用化学方法解码非规范多聚泛素链
基本信息
- 批准号:10364631
- 负责人:
- 金额:$ 30.32万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-04-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:ATP phosphohydrolaseAffinityBindingBinding ProteinsBiochemicalBioinformaticsBiological AssayBiological ProcessBiotinBypassCell physiologyCellsChemicalsComplexCrosslinkerCryoelectron MicroscopyCyclooctenesDNADNA BindingDNA DamageDNA biosynthesisDNA replication forkDataDevelopmentEukaryotaEventGenerationsGoalsHealthHomologous GeneHumanIndividualInvestigationKnowledgeLabelLengthLesionLigationLinkLysineMass Spectrum AnalysisMediatingMethodsModelingModificationOrthologous GenePathway interactionsPolyubiquitinPolyubiquitinationProteinsReaderRegulationResistanceRoleSignal TransductionStreptavidinStructureSystemTechnologyTestingTherapeutic InterventionUV inducedUbiquitinUbiquitinationYeastsbiophysical propertiescrosslinkmulticatalytic endopeptidase complexmutantnovelnovel therapeutic interventionprotein degradationreconstitutionrecruitresponsestemthioether
项目摘要
Decoding the noncanonical polyubiquitin chains using chemical approaches
The ubiquitin system is crucial for the regulation of a wide range of cellular and organismal functions
essential for the human health. Although our knowledge of the ubiquitin system initially stems from the
investigation into proteasome-mediated protein degradation, it has become clear that ubiquitination is also
indispensible for many non-proteolytic functions. Many noncanonical ubiquitin chains are associated with
cellular functions other than protein degradation. The K63-linked polyubiquitin chain in DNA damage tolerance
has been found to be signal for the eukaryotic error-free lesion bypass. However, little is known as to how this
signal regulates the DNA damage tolerance pathways. The downstream proteins that decode the K63-
polyubiquitin signals on PCNA still need to be identified. In order to decipher the diverse cellular roles of
polyubiquitination, chemical approaches of generating polyubiquitinated proteins and noncanonical ubiquitin
chains with defined structure and length are needed. This will lead to the discovery of the downstream reader
proteins in DNA damage tolerance and other important cellular processes. It will also enable in-depth
biochemical and biophysical characterizations of the recognition of noncanonical polyubiquitin chains by the
reader proteins. This will broaden our understanding of the many biological processes regulated by
ubiquitination and furnish new pathways and targets amenable for therapeutic intervention.
用化学方法解码非规范多泛素链
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Zhihao Zhuang其他文献
Zhihao Zhuang的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Zhihao Zhuang', 18)}}的其他基金
Investigating autophagic degradation of tau mediated by polyubiquitination
研究多泛素化介导的 tau 自噬降解
- 批准号:
10432378 - 财政年份:2022
- 资助金额:
$ 30.32万 - 项目类别:
Developing a cell-based high throughput screening for USP15 deubiquitinase inhibitor discovery
开发基于细胞的高通量筛选以发现 USP15 去泛素酶抑制剂
- 批准号:
10287750 - 财政年份:2021
- 资助金额:
$ 30.32万 - 项目类别:
NIH ADMINISTRATIVE SUPPLEMENT AUTOMATED PEPTIDE SYNTHESIZER ZHUANG
NIH 行政补充自动肽合成仪庄
- 批准号:
10387411 - 财政年份:2019
- 资助金额:
$ 30.32万 - 项目类别:
Decoding the non-canonical polyubiquitin chains using chemical approaches
使用化学方法解码非规范多聚泛素链
- 批准号:
9902465 - 财政年份:2019
- 资助金额:
$ 30.32万 - 项目类别:
Developing ubiquitin chain- and target-specific deubiquitinase probes
开发泛素链和靶标特异性去泛素酶探针
- 批准号:
8852722 - 财政年份:2014
- 资助金额:
$ 30.32万 - 项目类别:
Developing ubiquitin chain- and target-specific deubiquitinase probes
开发泛素链和靶标特异性去泛素酶探针
- 批准号:
8772352 - 财政年份:2014
- 资助金额:
$ 30.32万 - 项目类别:
Regulation and specificity of deubiquitylating enzyme complex
去泛素化酶复合物的调节和特异性
- 批准号:
8618909 - 财政年份:2012
- 资助金额:
$ 30.32万 - 项目类别:
Regulation and specificity of deubiquitylating enzyme complex
去泛素化酶复合物的调节和特异性
- 批准号:
8297146 - 财政年份:2012
- 资助金额:
$ 30.32万 - 项目类别:
Regulation and specificity of deubiquitylating enzyme complex
去泛素化酶复合物的调节和特异性
- 批准号:
8464161 - 财政年份:2012
- 资助金额:
$ 30.32万 - 项目类别:
Regulation and specificity of deubiquitylating enzyme complex
去泛素化酶复合物的调节和特异性
- 批准号:
9014551 - 财政年份:2012
- 资助金额:
$ 30.32万 - 项目类别:
相似海外基金
Applications of Deep Learning for Binding Affinity Prediction
深度学习在结合亲和力预测中的应用
- 批准号:
2887848 - 财政年份:2023
- 资助金额:
$ 30.32万 - 项目类别:
Studentship
Metalloenzyme binding affinity prediction with VM2
使用 VM2 预测金属酶结合亲和力
- 批准号:
10697593 - 财政年份:2023
- 资助金额:
$ 30.32万 - 项目类别:
Building a binding community - Capacity and capability for affinity and kinetic analysis of molecular interactions.
建立结合社区 - 分子相互作用的亲和力和动力学分析的能力和能力。
- 批准号:
MR/X013227/1 - 财政年份:2022
- 资助金额:
$ 30.32万 - 项目类别:
Research Grant
Using dynamic network models to quantitatively predict changes in binding affinity/specificity that arise from long-range amino acid substitutions
使用动态网络模型定量预测由长程氨基酸取代引起的结合亲和力/特异性的变化
- 批准号:
10797940 - 财政年份:2022
- 资助金额:
$ 30.32万 - 项目类别:
Using dynamic network models to quantitatively predict changes in binding affinity/specificity that arise from long-range amino acid substitutions
使用动态网络模型定量预测由长距离氨基酸取代引起的结合亲和力/特异性的变化
- 批准号:
10502084 - 财政年份:2022
- 资助金额:
$ 30.32万 - 项目类别:
Using dynamic network models to quantitatively predict changes in binding affinity/specificity that arise from long-range amino acid substitutions
使用动态网络模型定量预测由长距离氨基酸取代引起的结合亲和力/特异性的变化
- 批准号:
10707418 - 财政年份:2022
- 资助金额:
$ 30.32万 - 项目类别:
Binding affinity of inositol phosphate analogs to protein toxin TcdB
磷酸肌醇类似物与蛋白质毒素 TcdB 的结合亲和力
- 批准号:
573604-2022 - 财政年份:2022
- 资助金额:
$ 30.32万 - 项目类别:
University Undergraduate Student Research Awards
Computational predictions of thermostability and binding affinity changes in enzymes
酶热稳定性和结合亲和力变化的计算预测
- 批准号:
2610945 - 财政年份:2021
- 资助金额:
$ 30.32万 - 项目类别:
Studentship
I-Corps: Physics-Based Binding Affinity Estimator
I-Corps:基于物理的结合亲和力估计器
- 批准号:
2138667 - 财政年份:2021
- 资助金额:
$ 30.32万 - 项目类别:
Standard Grant
Computational modelling and simulation of antibodies to enhance binding affinity of a potential Burkholderia pseudomallei therapeutic
抗体的计算模型和模拟,以增强潜在的鼻疽伯克霍尔德氏菌治疗剂的结合亲和力
- 批准号:
2750554 - 财政年份:2021
- 资助金额:
$ 30.32万 - 项目类别:
Studentship