NIH ADMINISTRATIVE SUPPLEMENT AUTOMATED PEPTIDE SYNTHESIZER ZHUANG

NIH 行政补充自动肽合成仪庄

• 批准号: 10387411
• 负责人: Zhihao Zhuang
• 金额: $ 6.97万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10387411
• 关键词: ATP phosphohydrolase; Administrative Supplement; Affinity; Binding; Binding Proteins; Biochemical; Bioinformatics; Biological Assay; Biological Process; Biotin; Bypass; Cell physiology; Cells; Chemicals; Complex; Crosslinker; Cryoelectron Microscopy; Cyclooctenes; DNA; DNA Binding; DNA Damage; DNA biosynthesis; DNA replication fork; Data; Development; Ensure; Eukaryota; Event; Generations; Goals; Homologous Gene; Human; Individual; Investigation; Label; Length; Lesion; Ligation; Link; Lysine; Mass Spectrum Analysis; Mediating; Methods; Modeling; Modification; Orthologous Gene; Parents; Pathway interactions; Peptide Synthesis; Peptides; Phase; Polyubiquitin; Polyubiquitination; Proteins; Reader; Research; Resistance; Role; Signal Transduction; Solid; Streptavidin; Structure; System; Technology; Testing; Therapeutic Intervention; UV induced; Ubiquitin; Ubiquitination; United States National Institutes of Health; Yeasts; crosslink; multicatalytic endopeptidase complex; mutant; novel; novel strategies; novel therapeutic intervention; protein degradation; reconstitution; recruit; response; success; thioether; unnatural amino acids

ABSTRACT This supplement is requested to support R01 GM129468: Decoding the non-canonical polyubiquitin chains using chemical approaches. The goal of the parent proposal is to develop new approaches for protein polyubiquitination and subsequent identification and characterization of readers of the noncanonical ubiquitin chains in DNA damage tolerance. The success of this proposal critically depends on the ability to generate modified ubiquitin species that contain reactive linker molecules or unnatural amino acids. Using automated solid-phase peptide synthesis (SPPS) to generate the critical ubiquitin species in sufficiently large quantity will ensure the success of proposed research.

抽象的 要求该补充支持R01 GM129468：解码非典型 使用化学方法的多泛素链。父母建议的目标是发展 蛋白质多泛素化以及随后的识别和表征的新方法 DNA损伤耐受性中非规范泛素链的读者。这个成功 提案严重取决于产生含有修改的泛素种类的能力 反应性接头分子或不自然的氨基酸。使用自动固相肽 合成（spps）以足够数量的大量生成临界泛素物种将确保 拟议研究的成功。

项目成果

Chemical methods for protein site-specific ubiquitination.

• DOI: 10.1039/d0cb00215a
• 发表时间: 2021-04-01
• 期刊: RSC chemical biology
• 影响因子: 4.1
• 作者: Gui W;Davidson GA;Zhuang Z
• 通讯作者: Zhuang Z