Epigenetic mechanisms governing genome partitioning and gene expression in germ cells

控制生殖细胞基因组划分和基因表达的表观遗传机制

基本信息

  • 批准号:
    10364657
  • 负责人:
  • 金额:
    $ 41.82万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-04-01 至 2024-03-31
  • 项目状态:
    已结题

项目摘要

Three-dimensional genome organization, in which the linear DNA sequence is partitioned into functionally distinct domains, is essential for the precise deployment of genetic information in every cell type. An increasing number of devastating disorders and diseases have recently been attributed to disruption of genome organization, yet the mechanistic underpinnings remain poorly understood, especially in vivo. In particular, how the activity of binding factors and sequence elements is translated to epigenetic states and three-dimensional genomic domains to govern gene expression remains mysterious. The germ line of C. elegans provides a relevant and experimentally advantageous in vivo model to dissect the mechanisms governing proper establishment of genomic domains with coordinated regulation. In this system, we investigate diverse large-scale gene regulatory mechanisms controlling piRNA biogenesis, X chromosome regulation, and transformation from the germline to the somatic fate. We have recently developed the ability to reliably purify germ nuclei at quantities sufficient for genomic analyses, including ChIP-seq, RNA-seq, and chromatin capture conformation assays. This achievement removes a major barrier in the field to dissect gene expression mechanisms in the germ line. With this new capacity, we will now characterize fundamental transcriptional and epigenetic mechanisms in wild type germ nuclei with unprecedented specificity. Using these data, we will now define key mechanisms governing the germline-to-soma transformation, the coordinated, germline-specific regulation of genomic domains containing thousands of piRNA genes, and the temporal and global regulation of X chromosome gene expression in the germ line. The successful completion of the proposed experiments will illuminate the poorly understood yet vitally important in vivo mechanisms by which large-scale genome organization influences gene expression, and vice versa.
三维基因组组织,其中线性DNA序列被分割成 功能不同的区域,对于在每个细胞中精确地部署遗传信息是必不可少的 打字。最近,越来越多的破坏性疾病和疾病被归因于 基因组组织的破坏,然而其机制基础仍然鲜为人知, 尤其是在活体内。尤其是结合因子和序列元件的活性如何被翻译成 表观遗传状态和控制基因表达残基的三维基因组结构域 很神秘。线虫的种系在体内提供了相关的和实验上有利的 用配位技术剖析基因组结构域正确建立机制的模型 监管。在这个系统中,我们研究了不同的大规模基因调控机制 PiRNA的生物发生,X染色体调控,以及从生殖系到体细胞命运的转变。 我们最近开发了可靠地提纯生殖核的能力,提纯的数量足以满足基因组 分析,包括芯片序列分析、RNA-序列分析和染色质捕获构象分析。这一成就 消除了该领域的一个主要障碍,以剖析生殖系中的基因表达机制。有了这个 新的能力,我们现在将描述野生动物的基本转录和表观遗传机制 具有前所未有的特异性的类型生殖核。使用这些数据,我们现在将定义关键机制 控制种系到胞体的转化,基因组的协调、种系特异性调节 包含数千个piRNA基因的结构域,以及X染色体的时间和全球调节 基因在生殖系中的表达。拟议中的实验的成功完成将说明 对大规模基因组组织的体内机制知之甚少但至关重要的机制 影响基因表达,反之亦然。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

VALERIE J REINKE其他文献

VALERIE J REINKE的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('VALERIE J REINKE', 18)}}的其他基金

Epigenetic mechanisms governing genome partitioning and gene expression in germ cells
控制生殖细胞基因组划分和基因表达的表观遗传机制
  • 批准号:
    9903402
  • 财政年份:
    2019
  • 资助金额:
    $ 41.82万
  • 项目类别:
Epigenetic mechanisms governing genome partitioning and gene expression in germ cells
控制生殖细胞基因组划分和基因表达的表观遗传机制
  • 批准号:
    10591487
  • 财政年份:
    2019
  • 资助金额:
    $ 41.82万
  • 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
  • 批准号:
    9207005
  • 财政年份:
    2014
  • 资助金额:
    $ 41.82万
  • 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
  • 批准号:
    8795206
  • 财政年份:
    2014
  • 资助金额:
    $ 41.82万
  • 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
  • 批准号:
    9000710
  • 财政年份:
    2014
  • 资助金额:
    $ 41.82万
  • 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
  • 批准号:
    8612654
  • 财政年份:
    2014
  • 资助金额:
    $ 41.82万
  • 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
  • 批准号:
    6574542
  • 财政年份:
    2003
  • 资助金额:
    $ 41.82万
  • 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
  • 批准号:
    6845652
  • 财政年份:
    2003
  • 资助金额:
    $ 41.82万
  • 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
  • 批准号:
    6692191
  • 财政年份:
    2003
  • 资助金额:
    $ 41.82万
  • 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
  • 批准号:
    7172689
  • 财政年份:
    2003
  • 资助金额:
    $ 41.82万
  • 项目类别:

相似海外基金

Collaborative Research: Using Adaptive Lessons to Enhance Motivation, Cognitive Engagement, And Achievement Through Equitable Classroom Preparation
协作研究:通过公平的课堂准备,利用适应性课程来增强动机、认知参与和成就
  • 批准号:
    2335802
  • 财政年份:
    2024
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Standard Grant
Collaborative Research: Using Adaptive Lessons to Enhance Motivation, Cognitive Engagement, And Achievement Through Equitable Classroom Preparation
协作研究:通过公平的课堂准备,利用适应性课程来增强动机、认知参与和成就
  • 批准号:
    2335801
  • 财政年份:
    2024
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Standard Grant
A Longitudinal Study of the Relationship between Participation in a Comprehensive Exercise Program and Academic Achievement
参加综合锻炼计划与学业成绩之间关系的纵向研究
  • 批准号:
    24K14615
  • 财政年份:
    2024
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Collaborative Research: Characterizing Best Practices of Instructors who Have Narrowed Performance Gaps in Undergraduate Student Achievement in Introductory STEM Courses
合作研究:缩小本科生 STEM 入门课程成绩差距的讲师的最佳实践
  • 批准号:
    2420369
  • 财政年份:
    2024
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Standard Grant
Collaborative Research: Using Adaptive Lessons to Enhance Motivation, Cognitive Engagement, And Achievement Through Equitable Classroom Preparation
协作研究:通过公平的课堂准备,利用适应性课程来增强动机、认知参与和成就
  • 批准号:
    2335800
  • 财政年份:
    2024
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Standard Grant
WTG: Diffusion of Research on Supporting Mathematics Achievement for Youth with Disabilities through Twitter Translational Visual Abstracts
WTG:通过 Twitter 翻译视觉摘要传播支持残疾青少年数学成就的研究
  • 批准号:
    2244734
  • 财政年份:
    2023
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Standard Grant
The Impact of Emotional Experiences of Pride on Long-Term Goal Achievement Behaviors in Elite Athletes
骄傲的情感体验对优秀运动员长期目标实现行为的影响
  • 批准号:
    23K16740
  • 财政年份:
    2023
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Meta-Analysis of the Instructional-Relational Model of Student Engagement and Math Achievement: A Moderation and Mediation Approach
学生参与度和数学成绩的教学关系模型的元分析:一种调节和中介方法
  • 批准号:
    2300738
  • 财政年份:
    2023
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Standard Grant
Improving maths achievement in children with speech, language, and communication needs through 'collaborative vocabulary teaching'
通过“协作词汇教学”提高有言语、语言和交流需求的儿童的数学成绩
  • 批准号:
    2890475
  • 财政年份:
    2023
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Studentship
HSI Institutional Transformation Project: Retention and Achievement for Introductory STEM English Learners (RAISE)
HSI 机构转型项目:STEM 英语入门学习者的保留和成就 (RAISE)
  • 批准号:
    2225178
  • 财政年份:
    2023
  • 资助金额:
    $ 41.82万
  • 项目类别:
    Continuing Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了