Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
基本信息
- 批准号:8795206
- 负责人:
- 金额:$ 31.64万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-02-01 至 2018-01-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectArchitectureAutomobile DrivingBerylliumBindingBinding SitesBruck-de Lange syndromeC. elegans genomeCCCTC-binding factorCaenorhabditis elegansCell NucleusCellsChIP-seqChromatinChromatin LoopChromosomesCodeComplexDNA SequenceDataDevelopmentDiseaseElectron Transport Complex IIIElementsEnvironmentEnzymesEpigenetic ProcessEventExhibitsGene ExpressionGenesGeneticGenomeGenomicsGerm CellsGerm LinesHandHealthHistonesIndividualIntestinesLocationMapsMethodsMolecular ConformationMutateOrganismPatternPolymerasePropertyRNA Polymerase IIRNA Polymerase IIIRegulationRoberts-SC phocomelia syndromeSignal TransductionSiteSmall RNASpecificityTechniquesTissue-Specific Gene ExpressionTissuesTranslatingTriad Acrylic ResinUntranslated RNAWorkbasecell typechromatin immunoprecipitationchromatin modificationcohesindeep sequencingdevelopmental diseasegenetic informationgenome-widegenomic toolshistone modificationin vivoin vivo ModelinnovationnovelpiRNAresearch studysegregationtranscription factortranscription factor TFIIIC
项目摘要
DESCRIPTION (provided by applicant):
Three-dimensional genome organization, in which the linear DNA sequence is partitioned into functionally distinct domains, is essential for the precise deployment of genetic information in every cell type. An increasing number of devastating disorders and diseases have recently been attributed to disruption of genome organization, yet the mechanistic underpinnings remain poorly understood, especially in vivo. In particular, how the activity of binding factors and sequence elements is translated to epigenetic states and three-dimensional genomic domains to govern gene expression remains mysterious. C. elegans provides an experimentally advantageous in vivo model to dissect the mechanisms governing proper establishment of genomic domains. Data in hand points to a novel mechanism in which a sequence-specific factor (SNAP190) acts with components of the RNA polymerase III complex to define a large genomic domain exhibiting tissue-specific, coordinated regulation of thousands of noncoding small RNAs. In this proposal, the powerful genetic and genomic tools of C. elegans will be employed to investigate how SNAP190, RNA polymerase III, cohesin, and other candidate factors, interact with chromatin states (Aim 1), chromosomal looping (Aim 2), and cis- elements (Aim 3) to define the boundaries of, and regulate gene expression within, this unique genomic domain. The successful completion of the proposed experiments will illuminate the poorly understood yet vitally important mechanisms driving large-scale genome organization in vivo.
描述(由申请人提供):
三维基因组组织,其中线性DNA序列被划分为功能不同的域,对于在每种细胞类型中精确部署遗传信息至关重要。最近,越来越多的破坏性疾病和疾病被归因于基因组组织的破坏,但其机制基础仍然知之甚少,特别是在体内。特别是,结合因子和序列元件的活性如何被翻译成表观遗传状态和三维基因组结构域来控制基因表达仍然是个谜。C. elegans提供了一种实验上有利的体内模型来剖析控制基因组结构域正确建立的机制。手头的数据指出了一种新的机制,其中序列特异性因子(SNAP 190)与RNA聚合酶III复合物的组分一起作用,以定义一个大的基因组结构域,该结构域表现出组织特异性,协调调节数千个非编码小RNA。在这个建议中,强大的遗传和基因组工具的C。线虫将被用来研究SNAP 190、RNA聚合酶III、粘着蛋白和其他候选因子如何与染色质状态(Aim 1)、染色体成环(Aim 2)和顺式元件(Aim 3)相互作用,以确定这一独特基因组结构域的边界,并调节其内的基因表达。拟议实验的成功完成将阐明鲜为人知但至关重要的机制,推动大规模基因组组织在体内。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('VALERIE J REINKE', 18)}}的其他基金
Epigenetic mechanisms governing genome partitioning and gene expression in germ cells
控制生殖细胞基因组划分和基因表达的表观遗传机制
- 批准号:
10364657 - 财政年份:2019
- 资助金额:
$ 31.64万 - 项目类别:
Epigenetic mechanisms governing genome partitioning and gene expression in germ cells
控制生殖细胞基因组划分和基因表达的表观遗传机制
- 批准号:
9903402 - 财政年份:2019
- 资助金额:
$ 31.64万 - 项目类别:
Epigenetic mechanisms governing genome partitioning and gene expression in germ cells
控制生殖细胞基因组划分和基因表达的表观遗传机制
- 批准号:
10591487 - 财政年份:2019
- 资助金额:
$ 31.64万 - 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
- 批准号:
9207005 - 财政年份:2014
- 资助金额:
$ 31.64万 - 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
- 批准号:
9000710 - 财政年份:2014
- 资助金额:
$ 31.64万 - 项目类别:
Deciphering mechanisms governing functional partitioning of the C. elegans genome
破译控制线虫基因组功能分区的机制
- 批准号:
8612654 - 财政年份:2014
- 资助金额:
$ 31.64万 - 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
- 批准号:
6574542 - 财政年份:2003
- 资助金额:
$ 31.64万 - 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
- 批准号:
6845652 - 财政年份:2003
- 资助金额:
$ 31.64万 - 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
- 批准号:
6692191 - 财政年份:2003
- 资助金额:
$ 31.64万 - 项目类别:
MAP kinase signal specificity in the C. elegans germline
线虫种系中的 MAP 激酶信号特异性
- 批准号:
7172689 - 财政年份:2003
- 资助金额:
$ 31.64万 - 项目类别:
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