Applied Research - Project 1

项目摘要 几十年来，小鼠一直是研究人类疾病的首要动物模型， 有机会探索那些难以直接在人类身上进行研究的实验问题。作为 结果，获得了各种临床条件的关键见解。在进步的推动下， 技术和国际努力，系统地敲除所有的蛋白质编码基因， 基因工程小鼠的数量在过去20年中迅速增加。的 突变小鼠的维持和分布最初由个体负责， 调查人员，这是一个巨大的负担，在成本，劳动力和空间方面。1999年，NIH 认识到需要一种资源来保护对突变小鼠品系的投资， 接触所有生物医学研究人员，并建立了突变小鼠区域资源中心 （MMRRC）。自1999年以来，妇女、农村和儿童权利委员会的中心目标一直是与 MMRRC联盟合作伙伴开发和维护高标准的进口，再衍生， 冷冻保存和将突变小鼠品系分发给生物医学研究者。MMRRC- 在下一个项目期间，联合会将继续为实现联合会的目标作出贡献， 实现以下具体目标，致力于继续发挥其作为冷冻档案馆的成功作用， 配送中心，并结合研究目标，协同和扩展的价值， 资源：1）简化和改善操作程序，以增加进口，分销， 小鼠品系的冷冻库。2)建立、维护和分发一个全面的冷冻档案， 协作交叉（CC）资源。3)开发和传播小鼠遗传学计算工具 质量控制和基因组学研究，这将提高表型再现性。4)调查相互作用 遗传学和环境对实验变异性和再现性的影响。 !

PROJECT SUMMARY For decades, the mouse has been the premier animal model for studies of human disease, providing the opportunity to explore experimental questions that are intractable for direct study in humans. As a result, key insights have been obtained for a variety of clinical conditions. Spurred by advances in technology and international efforts to systematically knock out all of the protein coding genes in the genome, the number of genetically engineered mice has expanded rapidly in the past 20 years. The responsibility for maintenance and distribution of mutant mice was initially placed on individual investigators, which was a tremendous burden in terms of cost, labor and space. In 1999, the NIH recognized the need for a resource to protect the investment in mutant mouse strains and ensure open access to all biomedical researchers and established the Mutant Mouse Regional Resource Centers (MMRRC). Since 1999, the central goal of the MMRRC-UNC has been to work collectively with MMRRC consortium partners to develop and maintain high standards for importation, rederivation, cryopreservation, and distribution of mutant mouse strains to biomedical investigators. The MMRRC- UNC will continue to contribute to the goals of the Consortium in the next project period by implementing the following Specific Aims devoted to continuing its successful role as a cryoarchive and distribution center and incorporating research goals that synergize with and extend the value of the resource: 1) Streamline and improve operating procedures to increase importation, distribution, and cryoarchive of mouse strains. 2) Establish, maintain, and distribute a comprehensive cryoarchive for the Collaborative Cross (CC) resource. 3) Develop and disseminate computational tools for mouse genetic QC and genomics research, which will enhance phenotypic reproducibility. 4) Investigate the interaction of genetics and environment on experimental variability and reproducibility. !