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Mouse Genetics Core

小鼠遗传学核心

基本信息

批准号：
10238907
负责人：
Fernando Pardo-Manuel de Villena
金额：
$ 32.23万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-08-05 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10238907
关键词：
Affect Alleles Animals Antiviral Agents Automobile Driving Bioinformatics CRISPR/Cas technology Candidate Disease Gene Catalogs Cell Line Cells Collaborations Communicable Diseases Complex Congenic Strain Custom Data Data Set Databases Development Disease Dissection Environment Experimental Designs Experimental Models Frequencies Funding Generations Genes Genetic Genetic Polymorphism Genetic Transcription Genetic Variation Genetic study Genome Genotype Goals Heritability Homeostasis Human Immune Immune Response Genes Immune response Immunogenetics Inbred Strain Inbred Strains Mice Individual Infection Informatics Knowledge Lung Mediation Modeling Molecular Mechanisms of Action Mouse Strains Mus Outcome Pathogenesis Pathway interactions Phenotype Population Population Heterogeneity Process Public Health Quantitative Trait Loci Resources Role System Technology Validation Variant Viral Virus Diseases Whole Organism Work analysis pipeline biodefense causal variant cell type cost efficient design emerging pathogen experience experimental analysis experimental study gene interaction genetic architecture genetic information genetic variant genome editing genomic data in vivo in vivo Model model development mouse genetics mouse model pathogen pathogenic virus programs response trait whole genome

项目摘要

Abstract Mouse models provide highly tractable models with which to assess, identify and mechanistically characterize the role of genetic variants on infectious disease responses. The Mouse Genetics Core has been at the forefront of designing experimental crosses, sequence datasets and other mouse resources with which to investigate and perturb polymorphic immune networks. Following the identification of polymorphic host genome regions, aberrant mouse lines, and discordant immune spreads or candidate genes and pathways from human studies (in close collaboration with the Projects and the Systems Genetics and Bioinformatics core), we will generate a variety of mouse experimental models for the Projects to further assess, identify and characterize these processes. These models will include F1 panels contrasting variant disease alleles, F2 mapping populations, and mouse strains with modified candidate genes. Concurrently, designing the appropriate experiments for the study of viral disease requires an understanding of the underlying immune composition of individuals. Therefore, the Core will identify and further characterize genetic variants impacting basal immune responses; both intra-cellular as well as at the whole-organism level. These data will be provided to the projects to enhance their assessment of antiviral and/or perturbed response.

抽象的小鼠模型提供了高度易于处理的模型，可用于评估、识别和机械表征遗传变异对传染病反应的作用。小鼠遗传学核心已在设计实验杂交、序列数据集和其他小鼠资源的最前沿研究和扰乱多态性免疫网络。多态性宿主鉴定后基因组区域、异常小鼠系以及不一致的免疫传播或候选基因和途径来自人类研究（与项目和系统遗传学和生物信息学密切合作 core），我们将为项目生成各种小鼠实验模型，以进一步评估、识别和描述这些过程的特征。这些模型将包括对比变异疾病等位基因的 F1 组、F2 绘制具有修饰候选基因的种群和小鼠品系。同时，设计研究病毒性疾病的适当实验需要了解潜在的免疫个体的组成。因此，核心将识别并进一步表征影响的遗传变异基础免疫反应；无论是在细胞内还是在整个生物体水平。这些数据将提供给项目以加强对抗病毒和/或扰乱反应的评估。