Colorectal tumorigenesis on a cricket powder-based diet versus diets based on more typical protein sources

基于蟋蟀粉的饮食与基于更典型蛋白质来源的饮食的结直肠肿瘤发生

基本信息

批准号：
10368084
负责人：
JOEL B MASON
金额：
$ 7.55万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-03-08 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10368084
关键词：
Adult Agriculture American American Cancer Society Amino Acids Animal Model Attenuated Biochemical Chemoprevention Colorectal Colorectal Cancer Colorectal Neoplasms Consensus Consumption Data Developed Countries Diet Dietary Fats Domestic Fowls Fatty acid glycerol esters Flour Food Fresh Water Funding Goals Gryllidae Insecta International Agencies Kilogram Laboratories Link Malignant Neoplasms Mammals Meat Milk Modeling Molecular North America Nutritional Obesity Palate Pathway interactions Play Powder dose form Preventive Processed Meats Production Property Proteins Public Health Research Risk Risk Factors Rodent Model Role Smoking Solid Source System Taste Perception Texture Traction United Nations World Health Organization anticancer research base cancer prevention cancer risk colon carcinogenesis colon tumorigenesis colorectal cancer prevention colorectal cancer risk cost dietary experimental study greenhouse gases high risk mouse model novel novel strategies nutritional approach preservation soy systematic review tumorigenesis

项目摘要

Although some debate continues over the precise strength of the scientific evidence, there remains a strong consensus that habitual consumption of processed meat is a prominent causal risk factor for colorectal cancer (CRC). Two recent systematic reviews have concluded that each additional 50 gram serving/day of processed meat conveys an 18-23% increased risk, a figure highly relevant to U.S. adults whose mean daily consumption of processed meat is 50-60 grams. Substituting a modest proportion of that meat with an alternative protein source with lesser risk could therefore have a major impact on reducing the societal burden of CRC. Although an important protein source in many cultures for centuries, only recently have select insect species begun to gain traction as an acceptable protein source in North America. Cricket powder, comprised of ground, whole roasted crickets, can be incorporated into many foodstuffs with little or no discernible change in taste, making it an excellent candidate for acceptance in a culture naïve to entomophagy. Moreover, not only is its protein content very high, but qualitatively it is a complete protein. Further, cricket powder’s unique array of physiochemical features indicate it may possess cancer-preventive properties. Nevertheless an examination of insect-based foodstuffs for the purpose of cancer prevention has never before been explored, so the scope of this exploratory proposal is highly consistent with the spirit of the R03 mechanism. Achieving protein requirements through entomophagy has the further advantage of promoting sustainable agriculture, a longstanding goal of the U.S. Department of Agriculture. We hypothesize that in a well-accepted animal model of CRC, tumorigenesis will be diminished by a diet whose protein is largely derived from cricket powder compared to one whose protein is derived from processed meat, and will be non-inferior to the tumorigenesis observed with diets whose protein is derived from the conventional non-meat sources, dairy and soy. Our long-term goal is to identify a novel approach to CRC prevention that is effective, non-invasive, well- tolerated, and low cost, and thus will have wide application both domestically and globally. A secondary long- term goal is to promote sustainable food systems. We propose to conduct a study in a murine model of obesity- promoted colorectal carcinogenesis to: 1) determine whether substitution with cricket powder attenuates the enhanced risk of colorectal neoplasia that accompanies the habitual consumption of processed meat, 2) examine whether tumorigenesis generated by a cricket powder-based diet is non-inferior to diets whose protein is derived from soy flour or whole milk solids, and 3) begin to delineate the biochemical, molecular, and cytokinetic pathways by which a cricket-powder based diet generates less tumorigenesis than a processed meat one. My laboratory has successfully pursued a wide array of research topics in the nutritional chemoprevention of colorectal carcinogenesis in various animal models for over 25 years, providing us with all the necessary expertise to conduct the proposed study expeditiously and with scientific rigor.

尽管关于科学证据的精确力量的一些辩论仍在继续，但仍有很强的辩论共识是，习惯食用加工肉是结直肠癌的重要因果风险因素（CRC）。最近的两项系统评论得出的结论是，每加工/天的每天又有50克服务肉传达了风险增长18-23％，这一数字与美国成年人高度相关，他们的日常消费平均消费加工肉为50-60克。用替代蛋白代替该肉的适度比例因此，风险较小的消息来源可能会对减少CRC的社会伯恩伯恩产生重大影响。尽管几个世纪以来，许多培养物中都是重要的蛋白质来源，但直到最近才有选择的昆虫物种在北美，开始成为一种可接受的蛋白质来源。由地面组成的板球粉整个烤板球，可以掺入许多食物中，味道几乎没有明显的变化，使其成为幼稚昆虫养殖文化中接受的绝佳候选者。而且，它不仅是蛋白质含量很高，但从定性上讲是一种完整的蛋白质。此外，板球粉的独特阵列理化特征表明它可能具有预防癌症的特性。然而为了预防癌症的目的，基于昆虫的食物从未探索过，因此该探索性建议与R03机制的精神高度一致。达到蛋白质通过昆虫的要求具有促进可持续农业的进一步优势，美国农业部的长期目标。我们假设在一个受过良好接受的动物模型中在CRC中，肿瘤发生将通过饮食降低，该饮食在很大程度上源自板球粉蛋白质源自加工肉的蛋白质，并且将不属于肿瘤发生饮食的蛋白质是从常规的非肉类来源（乳制品和大豆）得出的。我们的长期目标是确定一种有效，无创，良好的新型CRC预防方法容忍和低成本，因此在国内和全球都具有广泛的应用。次要长术语目标是促进可持续食品系统。我们建议在肥胖的鼠模型中进行一项研究 - 促进结直肠癌发生至：1）确定用板球粉取代是否会减弱增加了涉及加工肉习惯食用的结直肠瘤的风险，2）检查板球粉末饮食产生的肿瘤发生是否与蛋白质得出的饮食不属于从大豆面粉或全牛奶固体，3）开始描绘生化，分子和细胞力学板球饮食的途径比加工肉产生的肿瘤发生较少。我的实验室成功地追求了广泛的研究主题多种动物模型中的大肠癌发生了25年以上，为我们提供了所有必要的专业知识以迅速和科学严格进行拟议的研究。