SBIR TOPIC 403: HIGHLY ACCURATE SINGLE CELL SEQUENCING WITH SPATIAL RESOLUTION ENABLED BY NANOGENOMICS FOR CANCER RESEARCH

癌症患者的高死亡率主要与恶性细胞表现出的耐药性有关。通过SBIR项目，我们的目标是开发一种新技术，监测癌症耐药性，并及时筛选替代药物，以促进最有效的治疗过程。该技术将基于高度准确的单细胞测序技术，该技术可以使用纳米基因组学询问空间和时间分辨率。通过将纳米移液技术与NVIGEN高灵敏度磁性纳米颗粒核酸提取、靶点富集和NGS样品制备技术相结合，可以以空间和时间分辨率测量单细胞的纵向基因组学。我们的技术能够进行单细胞全转录组分析，并且比其他技术更敏感。 方法.在第一阶段研究中，我们将整合纳米移液和纳米颗粒单细胞测序工作流程，使其成为获得单细胞测序信息和以空间分辨率评估组织样本耐药性的有用工具。在第二阶段，我们将与一家领先的仪器和成像设备公司合作，自动化纳米移液管和纳米颗粒库的制备，用于具有空间分辨率的单细胞测序。

The high incidence of mortality in cancer patients is primarily related to drug resistance exhibited by the malignant cells. Through the SBIR project we aim to develop a novel technology that will monitor cancer drug resistance and provide timely screening of alternative drugs to facilitate the most effective course of treatment. This technology will be based on a highly accurate single cell sequencing technology that can interrogate both spatial and temporal resolution using nanogenomics. By integrating a nanopipetting technology that takes miniscule aliquot of single cell samples with NVIGEN highly sensitive magnetic nanoparticle enabled nucleic acid extraction, target enrichment and NGS sample preparation technology, longitudinal genomics of single cells can be measured with spatial and temporal resolution. Our technology enables single cell whole transcriptome analysis, and is orders of magnitude more sensitive than other methods. In phase I research, we will integrate the nanopipetting and nanoparticle single cell sequencing workflow, making it a useful tool for obtaining single cell sequencing information and assessing drug resistance in tissue samples with spatial resolution. In phase II, we will collaborate with a leading instrumentation and imaging devices company to automate the nanopipetting and nanoparticle library preparation for single cell sequencing with spatial resolution.