SBIR TOPIC 403: HIGHLY ACCURATE SINGLE CELL SEQUENCING WITH SPATIAL RESOLUTION ENABLED BY NANOGENOMICS FOR CANCER RESEARCH

SBIR 主题 403：通过纳米基因组学实现具有空间分辨率的高精度单细胞测序，用于癌症研究

• 批准号: 10374694
• 负责人: AIHUA FU
• 金额: $ 5.5万
• 依托单位: NVIGEN, INC.
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-16 至 2021-06-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10374694
• 关键词: Aliquot; Biological Assay; Cancer Patient; Cells; DNA; Drug Screening; Drug resistance; Exhibits; Genomics; Imaging Device; Incidence; Libraries; Magnetic nanoparticles; Measures; Methods; Monitor; Nucleic Acids; Pharmaceutical Preparations; Phase; Preparation; Research; Resolution; Sampling; Screening for cancer; Small Business Innovation Research Grant; Technology; Tissue Sample; anticancer research; base; cancer cell; cancer drug resistance; innovation; instrumentation; mortality; nanoparticle; new technology; single cell sequencing; temporal measurement; tool; transcriptome; tv watching

The high incidence of mortality in cancer patients is primarily related to drug resistance exhibited by the malignant cells. Through the SBIR project we aim to develop a novel technology that will monitor cancer drug resistance and provide timely screening of alternative drugs to facilitate the most effective course of treatment. This technology will be based on a highly accurate single cell sequencing technology that can interrogate both spatial and temporal resolution using nanogenomics. By integrating a nanopipetting technology that takes miniscule aliquot of single cell samples with NVIGEN highly sensitive magnetic nanoparticle enabled nucleic acid extraction, target enrichment and NGS sample preparation technology, longitudinal genomics of single cells can be measured with spatial and temporal resolution. Our technology enables single cell whole transcriptome analysis, and is orders of magnitude more sensitive than other methods. In phase I research, we will integrate the nanopipetting and nanoparticle single cell sequencing workflow, making it a useful tool for obtaining single cell sequencing information and assessing drug resistance in tissue samples with spatial resolution. In phase II, we will collaborate with a leading instrumentation and imaging devices company to automate the nanopipetting and nanoparticle library preparation for single cell sequencing with spatial resolution.

癌症患者的高死亡率主要与恶性肿瘤细胞的耐药性有关。通过SBIR项目，我们的目标是开发一种新技术来监测癌症耐药性，并及时筛选替代药物，以促进最有效的治疗过程。这项技术将基于一种高度精确的单细胞测序技术，该技术可以使用纳米基因组学来询问空间和时间分辨率。通过将纳米移液技术与NVIGEN高灵敏度磁性纳米颗粒核酸提取、靶富集和NGS样品制备技术相结合，可以在空间和时间分辨率下测量单细胞的纵向基因组学。我们的技术能够进行单细胞全转录组分析，并且比其他技术更敏感