Fluorescence tools that illuminate biology and inspire translation

A.总结（1 R35 GM 134963）。了解蛋白质和细胞器的机制 功能和功能障碍需要动态分析分子结构和相互作用 在活细胞中。我的实验室的一个长期目标是开发工具，揭示这些功能 元素，并将其应用于发现新的重要生物学和分子， 或有影响力的财产。我被复杂的多学科问题所吸引， 催化令人兴奋的非增量进展。我们不仅致力于开发工具， 并以创新的方式加以应用。荧光光谱法的特点突出， 本文所述的两个研究项目。在这两种情况下，我们开发的荧光工具， 先前的NIGMS支持已经发现了有影响力的新生物学;该应用程序继续这一点 开发和扩展新生物学以造福人类健康。

A. Summary (1R35GM134963). Understanding the mechanisms of protein and organelle function and dysfunction requires dynamic analysis of molecular structures and interactions in live cells. A long-term goal of my laboratory is to develop tools that reveal these functional elements and apply them to discover novel an significant biology and molecules with useful or influential properties. I am drawn to complex, multi-disciplinary problems where chemistry catalyzes exciting and non-incremental progress. We strive not simply to develop tools but also apply them in innovative ways. Fluorescence spectroscopy features prominently in the two research programs described herein. In both, fluorescent tools that we developed using prior NIGMS support have uncovered influential new biology; this application continues this development and expands the new biology to benefit human health.