Fluorescence tools that illuminate biology and inspire translation

A.摘要(1R35GM134963)。了解蛋白质和细胞器的作用机制 功能和功能障碍需要对分子结构和相互作用进行动态分析 在活细胞中。我的实验室的一个长期目标是开发能够揭示这些功能的工具 元素并将其应用于发现一种新的具有重要意义的生物学和分子 或有影响力的财产。我被复杂的、多学科的问题所吸引，在那里化学 促进激动人心的、非渐进的进步。我们不仅致力于开发工具，还致力于 也要以创新的方式应用它们。荧光光谱学的显著特征是 这里描述的两个研究计划。在这两种情况下，我们使用 之前的NIGMS支持已经发现了有影响力的新生物学；此应用程序继续此 发展和扩大新的生物学，以造福人类健康。

A. Summary (1R35GM134963). Understanding the mechanisms of protein and organelle function and dysfunction requires dynamic analysis of molecular structures and interactions in live cells. A long-term goal of my laboratory is to develop tools that reveal these functional elements and apply them to discover novel an significant biology and molecules with useful or influential properties. I am drawn to complex, multi-disciplinary problems where chemistry catalyzes exciting and non-incremental progress. We strive not simply to develop tools but also apply them in innovative ways. Fluorescence spectroscopy features prominently in the two research programs described herein. In both, fluorescent tools that we developed using prior NIGMS support have uncovered influential new biology; this application continues this development and expands the new biology to benefit human health.