Fluorescence tools that illuminate biology and inspire translation

项目总结： 了解蛋白质和细胞器功能障碍在疾病中的作用机制 需要对活细胞中的分子结构和相互作用进行详细和动态的分析。 我的实验室的长期目标是开发能够揭示这些功能元素的工具 并将其应用于发现有意义的生物学和/或设计具有有用特性的分子。我 我被困难、复杂、多学科的问题所吸引，在这些问题中，化学可以催化 激动人心的、非渐进的进步。我们不仅致力于开发工具，还致力于应用 以创新的方式获取新的和有影响力的知识。这个题目自然而然地引导着我们 进入不同和新兴的科学领域。荧光光谱学的显著特征是 这里描述的三个研究计划。第二部分，我们开发的荧光工具(部分 A)或创新(C部分)已经确定了有影响力的新生物学；这里是未来的工作 充分利用这些知识，着眼于翻译。在第三个阶段，工具开发正在进行- 正在进行(B部分)，但迄今的进展为非常令人兴奋的未来提供了明确的路线图 申请。

Project Summary: Understanding the mechanisms of protein and organelle function and dysfunction in disease requires a detailed and dynamic analysis of molecular structures and interactions in live cells. The long-term goal of my laboratory is to develop tools that reveal these functional elements and apply them to discover significant biology and/or design molecules with useful properties. I am drawn to difficult, complex, multi-disciplinary problems where chemistry can catalyze exciting and non-incremental progress. We strive not simply to develop tools, but also to apply them in innovative ways to acquire new and influential knowledge. This rubric leads us naturally into diverse and emerging scientific areas. Fluorescence spectroscopy features prominently in the three research programs described herein. In two, a fluorescent tool we developed (section A) or innovated (section C) has already identified influential new biology; here future work capitalizes on this knowledge with an eye to translation. In the third, tool development is on- going (section B) but progress-to-date provides a clear roadmap for very exciting future applications.