Fluorescence tools that illuminate biology and inspire translation

项目概要： 了解疾病中蛋白质和细胞器功能和功能障碍的机制 需要对活细胞中的分子结构和相互作用进行详细的动态分析。 我的实验室的长期目标是开发揭示这些功能元素的工具 并将其应用于发现重要的生物学和/或设计具有有用特性的分子。我 我被化学可以催化的困难、复杂、多学科的问题所吸引 令人兴奋的和非渐进的进展。我们不仅致力于开发工具，还致力于应用 他们以创新的方式获得新的和有影响力的知识。这个标题自然地引导我们 不同的新兴科学领域。荧光光谱法的特点突出， 本文所述的三个研究项目。第二，我们开发的荧光工具（第 A）或创新（C部分）已经确定了有影响力的新生物学;这里是未来的工作 利用这些知识进行翻译。在第三，工具开发是在- 正在进行（第B节），但迄今为止的进展为非常令人兴奋的未来提供了清晰的路线图 应用.

Project Summary: Understanding the mechanisms of protein and organelle function and dysfunction in disease requires a detailed and dynamic analysis of molecular structures and interactions in live cells. The long-term goal of my laboratory is to develop tools that reveal these functional elements and apply them to discover significant biology and/or design molecules with useful properties. I am drawn to difficult, complex, multi-disciplinary problems where chemistry can catalyze exciting and non-incremental progress. We strive not simply to develop tools, but also to apply them in innovative ways to acquire new and influential knowledge. This rubric leads us naturally into diverse and emerging scientific areas. Fluorescence spectroscopy features prominently in the three research programs described herein. In two, a fluorescent tool we developed (section A) or innovated (section C) has already identified influential new biology; here future work capitalizes on this knowledge with an eye to translation. In the third, tool development is on- going (section B) but progress-to-date provides a clear roadmap for very exciting future applications.