Unravelling small RNA regulatory networks to target and control bacteria.

解开小 RNA 调控网络以靶向和控制细菌。

• 批准号: DP150103715
• 负责人: Prof John Boyce
• 金额: $ 31.37万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2017-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150103715
• 关键词: Unravelling; small; RNA; regulatory; networks

Small RNA (sRNA) molecules are critical regulators of bacterial gene expression. These molecules control important phenotypes in the Gram-negative veterinary pathogen Pasteurella multocida. This project aims to identify the range of P. multocida sRNAs and to show how expression of these molecules changes under various growth conditions. Specifically, this project endeavours: to identify the mRNA targets of the sRNAs; to identify the mechanisms of sRNA-mRNA interaction; to build systems-biology models that describe the sRNA regulatory circuits; to design inhibitors capable of disrupting critical sRNA-mRNA interactions; and to use the new inhibitors to modulate specific phenotypes. The ability to precisely manipulate sRNA regulatory circuits could allow fine control of bacterial phenotypes and could be widely applicable.

小 RNA (sRNA) 分子是细菌基因表达的关键调节因子。这些分子控制革兰氏阴性兽医病原体多杀性巴斯德氏菌的重要表型。该项目旨在确定多杀性巴氏杆菌 sRNA 的范围，并展示这些分子的表达在不同生长条件下如何变化。具体来说，该项目致力于： 识别 sRNA 的 mRNA 靶点；确定 sRNA-mRNA 相互作用的机制；建立描述 sRNA 调节回路的系统生物学模型；设计能够破坏关键 sRNA-mRNA 相互作用的抑制剂；并使用新的抑制剂来调节特定的表型。精确操纵 sRNA 调节回路的能力可以实现对细菌表型的精细控制，并且可以广泛应用。