Resource

摘要（资源） Rat资源和研究中心（RRRC）为Rat提供了一个独特而重要的资源库服务。 通过进口、储存和分发大量的大鼠品系和胚胎干细胞， 线它确保有价值的模型得到保存，并提供给感兴趣的研究人员， 研究人员满足NIH的资源共享要求，减轻了个体研究人员的负担 确保动物模型在严格的遗传质量控制和健康状况下得到维护 监测标准，以提高实验的重现性。我们在下一个资助期内的目标是 继续改进和扩大区域资源研究与资源中心的职能和能力，以提供生物医学调查人员 与大鼠模型，胚胎干细胞，相关试剂，协议和服务，将促进他们的 research.我们将通过开发新技术和利用现有技术来提高质量， 资源的获取，冷冻保存，储存，质量控制，以及大鼠，精子，胚胎的分配 和胚胎干细胞我们将继续评估和改善我们的服务和沟通 与生物医学研究界合作。我们将扩大我们的服务，其中已经包括再衍生，殖民地 管理，广泛的遗传分析选项，使用CRISPR/Cas9技术创建模型，以及 按服务收费的微生物群表征。通过这种方式，RRRC将加强其服务能力 作为研究人员在生物医学研究中使用大鼠的急需的“一站式”资源。

Abstract (Resource) The Rat Resource and Research Center (RRRC) provides a unique and essential repository service to the research community by importing, storing and distributing a large number of rat strains and embryonic stem cell lines. It assures that valuable models are preserved and made available to interested investigators, allows researchers to satisfy NIH requirements for resource sharing, relieves individual investigators from the burden of animal distribution and ensures that models are maintained with rigorous genetic quality control and health monitoring standards to enhance experimental reproducibility. Our objective during the next funding period is to continue to improve and expand the functions and capabilities of the RRRC to provide biomedical investigators with the rat models, embryonic stem cells, related reagents, protocols and services that will facilitate their research. We will accomplish this by developing new and utilizing existing technologies to improve the quality of the resource’s acquisition, cryopreservation, storage, quality control, and distribution of rats, sperm, embryos and embryonic stem cells. We will continue to evaluate and improve our service delivery and our communication with the biomedical research community. We will expand our services which already include re-derivation, colony management, a wide array of genetic analysis options, model creation using CRISPR/Cas9 technology, and microbiota characterization on a fee-for-service basis. In this way, the RRRC will strengthen its ability to serve as a much-needed “one stop” resource for investigators using rats in biomedical research.