Rodent Cell Line Authentication

描述（由申请人提供）：本研究的长期目标是开发一种有效的方法来鉴定啮齿动物细胞系。细胞系的错误鉴定以及种间和种内交叉污染非常普遍，对生物医学研究产生了重大的负面影响。使用短串联重复序列（STR）分析已被证明是鉴定人类细胞系的有效手段，已为大量人类细胞系建立了STR遗传图谱，并且这些数据中的大部分可在ATCC STR图谱数据库中公开访问。目前不存在用于啮齿动物细胞系认证的等效资源。为了填补这一空白，本申请的具体目标是开发一种基于多重微卫星标记的测试，以生成小鼠和大鼠细胞系的独特遗传图谱，并建立这些图谱的数据库，用于啮齿动物细胞系认证。通过开发这些资源，将有可能为研究界提供一种具有成本效益和准确的啮齿动物细胞系鉴定方法。公共卫生相关性：细胞系的使用是基础生物医学研究的基石，确保细胞系真实性的能力对于确保指导影响公共卫生的科学进步的研究结果的完整性和有效性至关重要。建立一种用于生成啮齿动物细胞系的独特遗传图谱的方法，提供了一种快速、灵敏和准确的方法来验证研究实验室中使用的这些细胞系的真实性。

DESCRIPTION (provided by applicant): The long term goal of this research is to develop an efficient method to authenticate rodent cell lines. Misidentification as well as inter- and intra-species cross contamination of cell lines is widespread and has a substantial negative impact on biomedical research. The use of short tandem repeat (STR) analysis has been shown to be an effective means of authenticating human cell lines, STR genetic profiles have been established for a large number of human cell lines, and much of this data is publicly accessible in the ATCC STR Profile Database. Equivalent resources do not currently exist for authentication of rodent cell lines. In order to fill this gap, the specific aims of this application are to develop a multiplex microsatellite marker-based test to generate unique genetic profiles for mouse and rat cell lines and to build a database of these profiles to use for rodent cell line authentication. By developing these resources, it will be possible to provide a cost-effective and accurate method for rodent cell line authentication for the research community. PUBLIC HEALTH RELEVANCE: The use of cell lines is a cornerstone of basic biomedical research and the ability to ensure the authenticity of the cell lines is critical to ensuring the integrity and validity of the research results that guide scientific advances affecting public health. Establishing a method for generating unique genetic profiles for rodent cell lines provides a quick, sensitive, and accurate means to verify the authenticity of those cell lines being used in research laboratories.