Sox11 function in muscle stem cells
Sox11 在肌肉干细胞中的功能
基本信息
- 批准号:10379314
- 负责人:
- 金额:$ 3.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-04-01 至 2023-03-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAdultAgeAgingBehaviorBiological AssayBiologyCell Differentiation processCell LineCell ProliferationCell physiologyCellsDataDefectDevelopmentDifferentiation AntigensDuchenne muscular dystrophyEmbryoFamilyFiberGene ExpressionGene Expression RegulationGenesGeneticGenetic TranscriptionGoalsHMG-Box DomainsHindlimbIn VitroInjuryKnock-outKnowledgeLeadLuciferasesMediatingMethodologyMethylationMolecularMusMuscleMuscle DevelopmentMuscle satellite cellMyoblastsNatural regenerationNeuromuscular DiseasesPathway interactionsPhenotypePlayPopulationProliferatingPublishingRegenerative MedicineRegenerative capacityRegulationReporterReportingResearchResearch PersonnelResearch ProposalsRoleSOX11 geneScientistSecureSignal PathwaySignaling ProteinSkeletal MuscleSkeletal Muscle Satellite CellsTimeTissuesTrainingTranscription Initiation SiteTranscriptional RegulationWNT Signaling PathwayWorkadult stem cellage relatedagedbeta catenincancer cellcandidate markercareercell behaviorconditional knockoutexperimental studyfunctional declinegene regulatory networkimprovedin vivoinjuredinsightmouse modelmuscle regenerationmyogenesisneuromuscularnovelpostnatalprogenitorregeneration functionregenerativeregenerative therapyrepairedresponse to injuryself-renewalsingle-cell RNA sequencingstem cell fatestem cell functionstem cell populationstem cell therapystem cellstargeted treatmenttranscription factor
项目摘要
PROJECT SUMMARY
My career goal is to be an independent investigator researching the function of transcriptional regulation of
stem cells towards improving therapies for regenerative medicine. In this project, I will use mouse models to
study the regulation of a stem cell population called muscle satellite cells (MuSCs). The regenerative function
of MuSCs are controlled by gene regulatory networks (often transcriptional factors) that govern cell fate choice
among self-renewal, quiescence and/or differentiation. Our lab has recently employed single-cell RNA-
sequencing to profile gene expression of various cell populations during muscle regeneration. This assay led to
the identification of the transcription factor Sox11 as a candidate marker for MuSCs and a potential regulator of
MuSC differentiation. Studies suggest that Sox11 may regulate the expression of genes involved in the WNT/
β-catenin-signaling pathway, which has been shown to induce myogenesis during development and promote
differentiation of adult MuSCs in response to injury. To understand the genetic requirement and mechanism of
Sox11 in MuSCs, the proposal will address the following aims: (1) Determine the necessity for Sox11 in
skeletal muscle development and in response to injury. We will use conditional and inducible mouse models to
specifically knock-out Sox11 in muscle progenitors and in the adult MuSC pool. These experiments will allow
us to evaluate our hypothesis that Sox11 is essential for the differentiation of MuSCs in vivo. (2) Assess the
role Sox11 plays in WNT-signaling and determine if WNT activation can restore differentiation defects in
Sox11-KO myoblasts. This aim will evaluate our hypothesis that Sox11 regulates WNT-signaling pathway
genes to secure myoblast differentiation. While the essential function of Sox11 has been described in other
tissues, its role in myogenesis is unknown. This research proposal has the potential to identify Sox11 as novel
regulator of MuSC function via its transcriptional regulation of WNT-pathway components. This proposal will
undoubtedly provide excellent training in the field of gene regulation as it pertains to muscle stem cell function.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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STEPHANIE NICOLE OPRESCU其他文献
STEPHANIE NICOLE OPRESCU的其他文献
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{{ truncateString('STEPHANIE NICOLE OPRESCU', 18)}}的其他基金
The role of cis-regulatory elements in the inheritance of transcriptional memory through mitosis.
顺式调节元件在通过有丝分裂遗传转录记忆中的作用。
- 批准号:
10751881 - 财政年份:2023
- 资助金额:
$ 3.86万 - 项目类别:
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